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International Journal of Gynecological Pathology:
doi: 10.1097/PGP.0b013e31824ae6f2
Pathology of the Upper Genital Tract: Original Articles

Low-grade Serous Neoplasms of the Ovary With Transformation to High-grade Carcinomas: A Report of 3 Cases

Garg, Karuna M.D.; Park, Kay J. M.D.; Soslow, Robert A. M.D

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Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY

The authors declare no conflict of interest.

Address correspondence and reprint requests to Karuna Garg, MD, Department of Pathology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. E-mail: gargk@mskcc.org.

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Abstract

Ovarian borderline tumors usually occur in young patients and are associated with good clinical outcomes. A proportion of these tumors, particularly those with micropapillary features, may progress to low-grade serous carcinomas. Evolution of low-grade serous neoplasms to high-grade carcinomas is rare and infrequently reported. In this case report, we describe 3 cases of serous borderline tumor that progressed to high-grade carcinoma. Patient ages were 22, 35, and 47yr. Two patients were initially diagnosed with ovarian serous borderline tumor, and recurred in the pelvis 3 and 10 yr later with sarcomatoid carcinoma. The third patient presented with low-grade serous carcinoma arising in a micropapillary serous borderline tumor, and recurred with a carcinosarcoma 3 yr later. All 3 patients had an aggressive clinical course: 2 died of disease and 1 patient has disease progression on chemotherapy. Occasional low-grade serous tumors of the ovary may transform to high-grade carcinomas, which can occur many years after initial diagnosis. The presence of a high-grade component significantly worsens patient outcomes.

Ovarian neoplasms are divided into Types I and II. Type I carcinomas are low-grade tumors that develop from identifiable precursor lesions such as borderline tumors, and include mucinous, endometrioid, and low-grade serous carcinomas 1. Low-grade serous carcinomas often arise in a background of serous borderline tumors, particularly those with micropapillary features, and may show BRAF, KRAS, and ERBB2 mutations. Type II carcinomas are high-grade neoplasms that frequently show p53 mutations, the prototype being high-grade serous carcinoma. These are usually not associated with precursor borderline neoplasms and do not show the mutations observed in low-grade serous tumors 1,2. There are important prognostic and therapeutic differences between low-grade and high-grade serous carcinomas. Patients with low-grade serous carcinomas typically develop multiple recurrences over time and patient death can occur many years after the initial diagnosis. These tumors do not respond to conventional platinum-based chemotherapy used for ovarian tumors. High-grade serous carcinomas of the ovary, in contrast, usually respond to platinum-based chemotherapy, and the median survival is much shorter 3.

This proposed model of ovarian tumorigenesis effectively segregates ovarian serous neoplasms into 2 types with distinct morphologies, pathogenesis, molecular abnormalities, and clinical characteristics. In most cases, there is no overlap between these 2 types. Low-grade serous carcinomas, at recurrence, typically retain their original low-grade appearance. However, occasional cases of transformation or progression of low-grade serous tumors to high-grade carcinoma have been reported, but this is a rare phenomenon and current literature exists in the form of sporadic case reports.

These include cases of high-grade serous carcinomas associated with low-grade serous tumors diagnosed at initial presentation. Malpica et al. 4 found 1 case of high-grade serous carcinoma in association with a serous borderline tumor, representing 2% of their 50 high-grade serous carcinomas. Dehari et al. 5 reviewed 210 ovarian high-grade serous carcinomas and found 6 tumors associated with low-grade serous neoplasms (2.8%), 3 with serous borderline tumors, and 3 with invasive low-grade serous carcinomas. Quddus et al. 6, also reported a case of high-grade serous carcinoma arising in association with serous borderline tumor and low-grade serous carcinoma.

In some cases, a low-grade serous tumor recurred as high-grade carcinoma. Parker et al. 7 reported 2 cases of ovarian serous borderline tumor with early recurrences as high-grade carcinoma in axillary lymph nodes and hernia sac, 18 mo and 2 yr, respectively, after initial diagnosis. One tumor recurred as high-grade serous carcinoma, whereas the other recurrence consisted of poorly differentiated carcinoma with sarcomatoid areas.

There are also 4 reported cases of “sarcoma-like mural nodules” in association with serous borderline tumors 8–11. In all of these cases, the high-grade component consisted of sarcomatoid carcinoma.

In this report, we describe 3 additional cases of low-grade serous tumors that recurred as high-grade malignant neoplasms.

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PATIENT 1

This patient initially presented at the age of 22 yr with bilateral ovarian serous borderline tumors with noninvasive implants. She recurred twice within 3 yr as serous borderline tumor and noninvasive implants. She presented with a pelvic recurrence 7 yr later, which was diagnosed as low-grade serous carcinoma with invasive implants, and received 6 cycles of chemotherapy. She then presented 1 yr later at our institution with radiologic evidence of pelvic recurrence. She received multiple cycles of chemotherapy (combinations of doxorubicin, bevacizumab, and paclitaxel) with questionable response. She then presented with small bowel obstruction, and underwent palliative surgery with peritoneal biopsies. She died of disease 1 mo later.

The diagnosis of bilateral ovarian serous borderline tumors with noninvasive implants was confirmed. Only representative slides were submitted from bilateral ovaries for our review, and although focal micropapillary features were noted in 1 ovary, the findings did not meet the criteria of >5 mm micropapillary growth for an outright diagnosis of micropapillary borderline tumor 12. However, this evaluation was limited by our ability to examine only selected slides. Slides from the invasive implants and low-grade serous carcinoma were not available for our review.

Biopsies of the pelvic recurrence performed at our institution from the small bowel and abdomen showed a high-grade undifferentiated spindle cell malignant neoplasm, involving small bowel serosa and abdominal biopsies (Figs. 1A, B). There was no evidence of a low-grade serous component. Tumor cells were spindled and high-grade and the differential diagnosis included high-grade anaplastic carcinoma and sarcoma. By immunohistochemistry, the tumor cells displayed patchy staining for epithelial markers (cytokeratins and epithelial membrane antigen). All other stains, to exclude angiosarcoma, dedifferentiated liposarcoma, and mesothelioma, were negative. The tumor did not show abnormal expression of p53 by immunohistochemistry. On the basis of the morphology and the immunohistochemical profile, a diagnosis of sarcomatoid carcinoma was favored. The tumor was negative for BRAF and KRAS mutations.

Fig. 1
Fig. 1
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PATIENT 2

The patient was a 47-yr-old woman who presented with abdominal symptoms and was found to have bilateral ovarian serous borderline tumors. She then presented 3 yr later with a pelvic mass. She also had a history of lung adenocarcinoma, with brain metastasis. The patient died of disease 6 mo later.

Slides from the initial surgery showed bilateral ovarian serous borderline tumors with surface involvement. The ovarian tumors were well sampled, and there was no evidence of micropapillary features. Slides from the pelvic recurrence showed a high-grade spindled and epithelioid malignant neoplasm, without any evidence of a low-grade serous component (Figs. 2A, B). Immunohistochemistry showed that tumor cells were negative for multiple epithelial markers and sarcoma-specific markers. P53 stain could not be performed because of a lack of material. The tumor was diagnosed as a high-grade undifferentiated malignant neoplasm; sarcomatoid carcinoma was favored. The pelvic tumor was morphologically and immunophenotypically distinct from the lung primary.

Fig. 2
Fig. 2
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PATIENT 3

This patient presented at the age of 35 yr with abdominal symptoms and was diagnosed with right ovarian micropapillary low-grade serous carcinoma, Stage IIIC. She received intravenous and intraperitoneal chemotherapy. She then presented 2 yr later with rising CA-125 and underwent splenectomy and pancreatectomy. She presented within 1 yr with right axillary lymph node metastasis and underwent right axillary lymph node dissection. She subsequently received radiation and chemotherapy, but had evidence of disease progression on therapy.

Sections from the right ovary showed a low-grade serous carcinoma arising in a background of micropapillary serous borderline tumor (Figs. 3A, B). Tumor in extra-ovarian sites, including cul-de-sac and diaphragm, showed higher grade areas, with more cytologic atypia and mitoses. However, these foci did not meet the criteria for a diagnosis of high-grade serous carcinoma and were diagnosed as Grade 2 serous carcinoma (by application of the Shimizu-Silverberg criteria) 13. The splenectomy showed a poorly differentiated tumor with spindled and epithelioid appearance involving the splenic parenchyma and hilum (Fig. 3C). Immunohistochemistry for p53 on the splenic tumor was reportedly not abnormal. KRAS mutational analysis was also performed on the ovarian and splenic tumors, and both were negative. Finally, sections from the axillary lymph node metastasis showed overt features of carcinosarcoma (MMMT) with high-grade carcinoma and sarcoma components (Fig. 3D). A p53 stain in this tumor showed strong and diffuse overexpression.

Fig. 3
Fig. 3
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DISCUSSION

Progression in ovarian serous borderline tumors typically occurs in the form of low-grade serous carcinoma. This transformation is typically associated with increased disease tempo and poor prognosis. However, low-grade serous carcinomas tend to retain their low-grade histology at recurrent/metastatic sites 14. Evolution of borderline neoplasms or low-grade serous carcinoma to high-grade carcinoma is unusual, and described in sporadic case reports. In this case report, we described 3 additional cases of high-grade tumors arising in association with ovarian low-grade serous neoplasms. Table 1 summarizes all such reported cases in the English literature, including the current cases (Table 1).

Table 1
Table 1
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The high-grade carcinoma component may be seen at initial presentation, or in a subsequent recurrence. In all our cases, the high-grade component was evident only at the time of recurrence. The time period between initial diagnosis of low-grade serous tumor and progression to high-grade carcinoma is variable and ranges from 18 mo to as long as 10 yr in reported cases. The morphology of the high-grade component can also vary; 2 of 3 cases in our series recurred as sarcomatoid carcinoma and 1 as carcinosarcoma. High-grade serous carcinoma constituted the high-grade component in many reported cases. Some cases of serous borderline tumor with sarcomatoid carcinoma have been described as having mural-like nodules 8–10. Although mural nodules are well described in mucinous cystic tumors of the ovary, they are rare in serous borderline tumors 15.

Irrespective of morphology, the presence of a high-grade component significantly worsens patient outcomes. Two of the 3 patients in this report died of disease within 1 yr of diagnosis, whereas the third patient had evidence of disease progression while on chemotherapy. Unfavorable clinical outcomes were also seen in all such reported cases, most patients being dead due to disease or alive with disease (Table 1). Patients with ovarian serous borderline tumor generally have a good prognosis with overall survival and disease-free survival of 98% and 87% for Stage 1, and 91% and 65%, respectively, for patients who present at higher stages 14. Reported 5-yr survival rates range from 40% to 56% for low-grade serous carcinomas and 9% to 34% for high-grade serous carcinomas 4,16.

The mechanism of this transformation is not certain. Low-grade serous tumors show mutations in BRAF/KRAS/ERBB2, whereas high-grade serous carcinomas arise as a result of Tp53 mutations. BRAF and/or KRAS mutational analysis was performed in 2 of our cases and both were negative. Dehari and colleagues found identical KRAS mutations in the low-grade and high-grade components in 2 of their 6 combined high-grade and low-grade serous carcinomas, indicative of a clonal relationship. However, they did not find p53 mutations in any of the high-grade components of the 6 tumors. This raises the possibility that unlike conventional high-grade serous carcinomas, those that arise in this setting may not be associated with p53 mutations. However, Quddus and colleagues reported the presence of strong and diffuse p53 staining (an immunohistochemical staining pattern that correlates with Tp53 mutation) in the high-grade component of their combined low-grade and high-grade serous carcinoma 17. Therefore, the role of Tp53 mutation in this progression is not certain. Our cases also showed variable p53 staining results. The high-grade tumor from Patient 1 did not show p53 overexpression. The carcinosarcoma from Patient 3 showed p53 overexpression, whereas the preceding splenic tumor was reportedly p53 negative, suggesting that p53 mutation was acquired during transformation to high-grade carcinoma. Staining for p53 could not be performed in one case.

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CONCLUSIONS

Progression of ovarian serous borderline tumor and low-grade serous carcinoma to high-grade carcinoma is unusual, but can sometimes occur. The high-grade component may be seen at presentation or in a recurrence many years after the initial diagnosis. The high-grade component may be composed of high-grade serous carcinoma, sarcomatoid carcinoma, or carcinosarcoma. The presence of this component is associated with aggressive behavior and poor clinical outcomes. The mechanism of this transformation/progression is not clear.

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REFERENCES

1. Kurman RJ, Shih Ie M. The origin and pathogenesis of epithelial ovarian cancer: a proposed unifying theory. Am J Surg Pathol. 2010;34:433–43

2. Shih Ie M, Kurman RJ. Ovarian tumorigenesis: a proposed model based on morphological and molecular genetic analysis. Am J Pathol. 2004;164:1511–8

3. Singer G, Stohr R, Cope L, et al. Patterns of p53 mutations separate ovarian serous borderline tumors and low- and high-grade carcinomas and provide support for a new model of ovarian carcinogenesis: a mutational analysis with immunohistochemical correlation. Am J Surg Pathol. 2005;29:218–24

4. Malpica A, Deavers MT, Lu K, et al. Grading ovarian serous carcinoma using a two-tier system. Am J Surg Pathol. 2004;28:496–504

5. Dehari R, Kurman RJ, Logani S, et al. The development of high-grade serous carcinoma from atypical proliferative (borderline) serous tumors and low-grade micropapillary serous carcinoma: a morphologic and molecular genetic analysis. Am J Surg Pathol. 2007;31:1007–12

6. Quddus MR, Rashid LB, Hansen K, et al. High-grade serous carcinoma arising in a low-grade serous carcinoma and micropapillary serous borderline tumour of the ovary in a 23-year-old woman. Histopathology. 2009;54:771–3

7. Parker RL, Clement PB, Chercover DJ, et al. Early recurrence of ovarian serous borderline tumor as high-grade carcinoma: a report of two cases. Int J Gynecol Pathol. 2004;23:265–72

8. Gungor T, Altinkaya SO, Akbay S, et al. Malign mural nodules associated with serous ovarian tumor of borderline malignancy: a case report and literature review. Arch Gynecol Obstet. 2010;281:485–90

9. Andrews TD, Dutton PM, Beattie G, et al. Sarcomatoid carcinoma arising within a serous borderline ovarian tumour: a case report and practical approach to differential diagnosis. Histopathology. 2008;52:233–8

10. McCullough J, Batman P, Parker D. Sarcoma-like nodules in cystic ovarian tumours. J Clin Pathol. 1988;41:1028–9

11. De Rosa G, Donofrio V, De Rosa N, et al. Ovarian serous tumor with mural nodules of carcinomatous derivation (sarcomatoid carcinoma): report of a case. Int J Gynecol Pathol. 1991;10:311–8

12. Eichhorn JH, Bell DA, Young RH, et al. Ovarian serous borderline tumors with micropapillary and cribriform patterns: a study of 40 cases and comparison with 44 cases without these patterns. Am J Surg Pathol. 1999;23:397–409

13. Shimizu Y, Kamoi S, Amada S, et al. Toward the development of a universal grading system for ovarian epithelial carcinoma: testing of a proposed system in a series of 461 patients with uniform treatment and follow-up. Cancer. 1998;82:893–901

14. Longacre TA, McKenney JK, Tazelaar HD, et al. Ovarian serous tumors of low malignant potential (borderline tumors): outcome-based study of 276 patients with long-term (≥5-year) follow-up. Am J Surg Pathol. 2005;29:707–23

15. Provenza C, Young RH, Prat J. Anaplastic carcinoma in mucinous ovarian tumors: a clinicopathologic study of 34 cases emphasizing the crucial impact of stage on prognosis, their histologic spectrum, and overlap with sarcomalike mural nodules. Am J Surg Pathol. 2008;32:383–9

16. Seidman JD, Horkayne-Szakaly I, Cosin JA, et al. Testing of two binary grading systems for FIGO stage III serous carcinoma of the ovary and peritoneum. Gynecol Oncol. 2006;103:703–8

17. Yemelyanova A, Vang R, Kshirsagar M, et al. Immunohistochemical staining patterns of p53 can serve as a surrogate marker for TP53 mutations in ovarian carcinoma: an immunohistochemical and nucleotide sequencing analysis. Mod Pathol. 2011;24:1248–53

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Keywords:

Ovary; Serous borderline tumor; Low-grade serous carcinoma; Progression to high-grade carcinoma

©2012International Society of Gynecological Pathologists

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