Our earlier study showed that the autophagy gene Beclin 1 could affect cell proliferation in a cervical cancer HeLa cell line. In this study, we examined Beclin 1 protein expression in 81 specimens of cervical squamous carcinoma by immunohistochemistry. Meanwhile, we detected E6 and E7 genes of human papillomavirus 16 in these tissues by polymerase chain reaction. Beclin 1 expression significantly decreased in samples of malignant cervical cancer tissues than in those of normal or cervical intraepithelial neoplasia tissues. The expression of Beclin 1 was associated with pelvic lymph node metastasis and histological grade, but did not correlate with International Federation of Gynecology and Obstetrics stage, age, depth of cervical infiltration, tumor size, and gross type of cervical lesion. The expression of Beclin 1 was not obviously correlated with E6 and E7 genes statistically. Therefore, decreased expression of Beclin 1 may be related to tumorigenesis and the development of cervical cancer, but is not significantly relevant with human papillomavirus 16 infection.
Departments of Gynecology (Z-H.W.)
Pathology (T.B.), The First Hospital, Shanxi Medical University, Taiyuan
Department of Respiratory Medicine (L.X.)
Department of Neurology (Y.W.), The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing
School of Public Health (M-Q.C.), Xinjiang Medical University, Urumqi, Xinjiang, China
The research is supported by the National Natural Science Foundation of China (Grant No. 30740016), Shanxi Province Science Foundation for Youths (Grant No. 2008021046-2).
Zan-Hong Wan and Li Xu have contributed equally to the paper.
The authors declare that there are no conflicts of interest in this work.
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Address correspondence reprint request to Zan-Hong Wang, Department of Gynecology, The First Hospital, Shanxi Medical University, Taiyuan 030001, P.R. China. e-mail: firstname.lastname@example.org.