We describe the case of a 58-year-old man experiencing severe abdominal pain around the epigastric area that presents a positive Murphy sign. A spiral-shaped, gram-negative anaerobic bacterium was isolated from the bile. Attempts to identify the bacteria by an automated system based on biochemical testing were unsuccessful. Anaerobiospirillum succiniciproducens was identified by 16S ribosomal RNA gene amplification by polymerase chain reaction and subsequent analysis. The patient responded to a combination therapy based on amoxicillin/clavulanic and ciprofloxacin.