Autoimmune hemolytic anemia triggered by several different viral infections is a well-recognized entity; however, the role of coxsackievirus B in this condition is unknown. We report the case of a 22-year-old previously healthy woman who presented with an acute febrile illness, with prominent gastrointestinal symptoms, and an acute myocarditis. The patient received supportive therapy and was hemodynamically stabilized, but fever persisted for 35 days after the onset of her symptoms. During the third week of her hospitalization, the patient developed a severe autoimmune hemolytic anemia. Result of Coombs test was positive. Drugs, malignancy, and rheumatologic diseases were ruled out as causes of anemia. Serological examination results showed a 4-fold increase in the titers of coxsackievirus B5 when acute sera were compared with convalescent sera, demonstrating an acute infection. The anemia responded to high-dose steroids and intravenous immunoglobulin. Coombs test became negative. We postulate that this patient's acute infection by coxsackievirus triggered the autoimmune hemolytic anemia. Similar case reports are needed to further support the hypothesis of this association.