An unusual case of aspergillosis in the bladder with no evidence of disseminated or renal aspergillosis is presented. A 70-year-old man whose main complaints were dysuria, hematuria, and vomiting had undergone a cystoscopy, which revealed inflammation of the trigone and bladder neck. Histological analysis showed a high-grade transitional carcinoma, and results of a bladder tissue culture were positive for Aspergillus fumigatus. Isolated infection of the bladder due to Aspergillus species is rare, with only 1 case being previously reported. To our knowledge, this is the first case of aspergillosis in the bladder accompanying a bladder malignancy.