Linezolid belongs to a new class of antibiotics known as oxazolidinones. A 30-year-old healthy man with tibial osteomyelitis was treated with linezolid. Seven days after beginning linezolid, he developed a diffuse maculopapular rash and acute renal failure. Renal biopsy was diagnostic of acute interstitial nephritis (AIN). Withdrawal of linezolid, short-term hemodialysis, and corticosteroid therapy led to resolution of symptoms and eventual recovery of renal function. This is the second case report of linezolid-induced AIN.