You could be reading the full-text of this article now if you...

If you have access to this article through your institution,
you can view this article in

Biological Response to -Tricalcium Phosphate/Calcium Sulfate Synthetic Graft Material: An Experimental Study

Leventis, Minas D. DDS, MS, PhD*; Fairbairn, Peter BDS; Dontas, Ismene DVM, PhD; Faratzis, Gregory MD, DDS, MS, PhD§; Valavanis, Konstantinos D. DDS; Khaldi, Lubna MD, PhD; Kostakis, George MD, DDS, MS, PhD*; Eleftheriadis, Efstathios MD, DDS, PhD#

Implant Dentistry:
doi: 10.1097/ID.0000000000000030
Basic and Clinical Research

Purpose: The aim of this study was to evaluate the effect of a biphasic synthetic bone graft material composed of β-tricalcium phosphate (β-TCP) and calcium sulfate (CS) in 12 New Zealand rabbits.

Materials and Methods: A circular bicortical critical-size cranial defect was created in each of 12 rabbits. The defects were grafted with β-TCP/CS. Animals were euthanized at 3 and 6 weeks. Harvested tissue specimens were evaluated histologically and histomorphometrically. Parameters associated with new bone formation and graft resorption were measured and calculated. The results were statistically analyzed using the Mann-Whitney test.

Results: Our data demonstrated the biocompatibility of synthetic β-TCP/CS, as no inflammatory response was observed, and no fibrosis was developed between the graft particles and the newly formed bone. Moreover, β-TCP/CS acted as an osteoconductive scaffold that allowed a significant bone regeneration and graft biodegradation with time.

Conclusion: In this animal model, synthetic β-TCP/CS proved to be a biocompatible, osteoconductive, and bioresorbable bone graft substitute.

Author Information

*Clinical Assistant, Department of Oral and Maxillofacial Surgery, Dental School, University of Athens, Greece.

Visiting Professor, Department of Periodontology and Implant Dentistry, School of Dentistry, University of Detroit Mercy, Detroit, MI.

Professor of Experimental Surgical Research, School of Medicine, University of Athens, Athens, Greece.

§Maxillofacial Surgeon, Department of Maxillofacial Surgery, Greek Anticancer Institute, St Savvas Hospital, Athens, Greece.

Professor, Department of Oral Surgery, University of Naples Federico II, Naples, Italy.

Pathologist, Laboratory for Research of the Musculoskeletal System “Th. Garofalidis”, Medical School, University of Athens, Athens, Greece.

#Associate Professor, Department of Oral and Maxillofacial Surgery, Dental School, University of Athens, Athens, Greece.

Reprint requests and correspondence to: Minas D. Leventis, DDS, MS, PhD, Department of Oral and Maxillofacial Surgery, Dental School, University of Athens, 2 Thivon Street, 115 27 Athens, Greece, Phone: +30-6937391769, Fax: +30-2106424400, E-mail:

© 2014 by Lippincott Williams & Wilkins, Inc.