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Peptide-15 Changes miRNA Expression in Osteoblast-Like Cells

Palmieri, Annalisa PhD*; Pezzetti, Furio PhD†; Brunelli, Giorgio MD‡; Martinelli, Marcella PhD§; Lo Muzio, Lorenzo MD∥; Scarano, Antonio DDS¶; Degidi, Marco MD#; Piattelli, Adriano MD**; Carinci, Francesco MD††

doi: 10.1097/ID.0b013e318166d182
Basic and Clinical Research

Purpose: Peptide-15 (P-15) is an analog of the cell-binding domain of collagen. P-15 has been shown to facilitate physiological process in a way similar to collagen, to serve as anchorage for cells, and to promote the binding, migration, and differentiation of cells. However, how P-15 alters osteoblast activity to promote bone formation is poorly understood. We therefore attempted to address this question by using microarray techniques to investigate the microRNA (miRNA) expression in osteoblasts exposed to P-15.

Materials: The miRNA oligonucleotide microarray provides a novel method to carry out genome-wide miRNA profiling in human samples. By using miRNA microarrays containing 329 probe designed from human miRNA sequence, we identified in osteoblast-like cells line (MG-63) cul-tured with P-15 several miRNA whose expression is significantly modified.

Results: We identified 11 up-regulated miRNA (i.e., mir-337, mir-15b, mir-377, mir-100, mir-148a, mir-125a, mir-199a, mir-221, mir-let-7d, mir-92, mir-23b) and six down-regulated miRNA (i.e., mir-422a, mir-19a, mir-224, mir-145, mir-22, mir-29a).

Conclusion: The data reported are, to our knowledge, the first on translation regulation in osteoblasts exposed to P-15. They can be relevant to better understand the molecular mechanism of bone regeneration and can serve as a model for comparing other materials with similar clinical effects.

*Post Doctoral Fellow, Institute of Histology, University of Bologna and Center of Molecular Genetics, CARISBO Foundation, Bologna, Italy.

†Associate Professor, Institute of Histology, University of Bologna and Center of Molecular Genetics, CARISBO Foundation, Bologna, Italy.

‡Senior Lecturer, Department of DMCCC, Section of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy.

§Assistant Professor, Institute of Histology, University of Bologna and Center of Molecular Genetics, CARISBO Foundation, Bologna, Italy.

∥Full Professor, Dental Clinic, University of Foggia, Foggia, Italy.

¶Assistant Professor, Dental Clinic, University of Chieti, Chieti, Italy.

#Senior Lecturer, Dental Clinic, University of Bologna, Bologna, Italy.

**Full Professor, Dental Clinic, University of Chieti, Chieti, Italy.

††Associate Professor, Department of DMCCC, Section of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy.

Reprint requests and correspondence to:

Francesco Carinci, MD; Department of DMCCC; Section of Maxillofacial Surgery; University of Ferrara; Corso Giovecca, 203; 44100 Ferrara, Italy; Phone/Fax: +39-0532-291582; E-mail: crc@unife.it; Web: www.carinci.org

© 2008 Lippincott Williams & Wilkins, Inc.