Skip Navigation LinksHome > May 2014 - Volume 24 - Issue 4 > S-Phase Cell Cycle Arrest, Apoptosis, and Molecular Mechanis...
International Journal of Gynecological Cancer:
doi: 10.1097/IGC.0000000000000105
Basic Science

S-Phase Cell Cycle Arrest, Apoptosis, and Molecular Mechanisms of Aplasia Ras homolog Member I–Induced Human Ovarian Cancer SKOV3 Cell Lines

Zhu, Qiaoying MD*†; Hu, Jianming MD; Meng, Huijuan MD; Shen, Yufei MD*†; Zhou, Jinhua MD; Zhu, Zhihong MD

Open Access
Collapse Box

Abstract

Objective: Aplasia Ras homolog member I (ARHI) is associated with human ovarian cancer (HOC) growth and proliferation; however, the mechanisms are unclear. The purpose of this study was to investigate ARHI effects in HOC SKOV3 cells.

Methods: We transfected SKOV3 cells with PIRES2-EGFP-ARHI and measured growth inhibition rates, cell cycle distribution, apoptosis rates, and expression of P-STAT3 (phosphorylated signal transduction and activators of transcription 3) and P-ERK (phosphorylated extracellular signal regulated protein kinase).

Results: Our data showed significant inhibition of growth, significantly increased S-phase arrest and apoptosis rates, and reduction of P-STAT3 and P-ERK1/2 expression levels.

Conclusions: We propose the mechanism may involve ARHI-induced phosphorylation of ERK1/2 and STAT3 protein kinases, thereby blocking proliferation signaling pathways, to induce HOC SKOV3 apoptosis.

© 2014 by the International Gynecologic Cancer Society and the European Society of Gynaecological Oncology.

Login

Article Tools

Share

Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.

Connect With Us

Twitter
twitter.com/IJGConline

For additional oncology content, visit LWW Oncology Journals on Facebook.