Skip Navigation LinksHome > May 2014 - Volume 24 - Issue 4 > S-Phase Cell Cycle Arrest, Apoptosis, and Molecular Mechanis...
International Journal of Gynecological Cancer:
doi: 10.1097/IGC.0000000000000105
Basic Science

S-Phase Cell Cycle Arrest, Apoptosis, and Molecular Mechanisms of Aplasia Ras homolog Member I–Induced Human Ovarian Cancer SKOV3 Cell Lines

Zhu, Qiaoying MD*†; Hu, Jianming MD; Meng, Huijuan MD; Shen, Yufei MD*†; Zhou, Jinhua MD; Zhu, Zhihong MD

Open Access
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Objective: Aplasia Ras homolog member I (ARHI) is associated with human ovarian cancer (HOC) growth and proliferation; however, the mechanisms are unclear. The purpose of this study was to investigate ARHI effects in HOC SKOV3 cells.

Methods: We transfected SKOV3 cells with PIRES2-EGFP-ARHI and measured growth inhibition rates, cell cycle distribution, apoptosis rates, and expression of P-STAT3 (phosphorylated signal transduction and activators of transcription 3) and P-ERK (phosphorylated extracellular signal regulated protein kinase).

Results: Our data showed significant inhibition of growth, significantly increased S-phase arrest and apoptosis rates, and reduction of P-STAT3 and P-ERK1/2 expression levels.

Conclusions: We propose the mechanism may involve ARHI-induced phosphorylation of ERK1/2 and STAT3 protein kinases, thereby blocking proliferation signaling pathways, to induce HOC SKOV3 apoptosis.

© 2014 by the International Gynecologic Cancer Society and the European Society of Gynaecological Oncology.


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