RhoC Mediates Invasion and Migration of CaSki Cells Through the Rho-Associated Serine-Threonine Protein Kinase 1 Signaling Pathway

Chen, Rong MD, MS*; Cheng, Yuan MD, MS*; Zhang, Youyi MD, PhD; Li, Zijian MD, PhD; Geng, Li MD, PhD*

International Journal of Gynecological Cancer:
doi: 10.1097/IGC.0000000000000053
Basic Science
Abstract

Objective: The small GTPase RhoC in human cancers is up-regulated and correlated with tumor metastasis. However, the role of Rho/Rho-associated serine-threonine protein kinase 1 (ROCK1) signaling pathway in human cervical cancer is still unclear. In this study, we examine the effects of RhoC and its major downstream target, ROCK1, on the invasion and migration of CaSki cells to investigate the role of RhoC/ROCK1 signaling pathway in the progression of cervical squamous cell carcinoma.

Methods: RhoC and ROCK1 protein expression in CaSki cells was detected by Western blotting. Scratch and transwell assays were carried out to assess the effects of RhoC on invasion and migration of CaSki cells. Cell viability was assayed by MTT test after adding the ROCK1 inhibitor Y-27632 to CaSki cells.

Results: Overexpression of RhoC protein in CaSki cells significantly increases ROCK1 expression and promotes cell invasion and migration compared with the control group (P < 0.05). However, in the inhibition of ROCK1 with Y-27632 in CaSki cells when RhoC was overexpressed, the rate of invasiveness and migration was reduced remarkably (P < 0.05), dropping to comparable levels as the control.

Conclusions: This study suggested that the activation of RhoC/ROCK1 signaling pathways is likely involved in the progression of cervical squamous cell carcinoma.

Author Information

*Department of Gynecology and Obstetrics, and †Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptide, Ministry of Health, Beijing, China.

Address correspondence and reprint requests to Dr Li Geng, PhD, Department of Gynecology and Obstetrics, Peking University Third Hospital, Huayuan-Bei Road No.49 Haidian District, Beijing 100191, China. E-mail: gengli1957@bjmu.edu.cn; Zijian Li, Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptide, Ministry of Health, Huayuan-Bei Road No.49 Haidian District, Beijing 100191, China. E-mail: lzjgy1995@163.com.

This work was supported by grants from the National Natural ScienceFoundation of China (nos. 11072006, 10772007, and 81070078) and National Basic Research Program of China (973 Program, 2013CB933702).

The authors declare no conflicts of interest.

Received July 29, 2013

Received in revised form October 31, 2013

Accepted October 31, 2013

© 2014 by the International Gynecologic Cancer Society and the European Society of Gynaecological Oncology.