HSD3B1 Is a Specific Trophoblast-Associated Marker Not Expressed in a Wide Spectrum of Tumors

Chou, Yuh-Yu MD*†; Jeng, Yung-Ming MD, PhD; Mao, Tsui-Lien MD

International Journal of Gynecological Cancer: February 2013 - Volume 23 - Issue 2 - p 343–347
doi: 10.1097/IGC.0b013e31827eaa78
Trophoblastic Cancer

Objective: Hydroxyl-δ-5-steroid dehydrogenase (HSD3B1) is an enzyme that catalyzes the oxidative conversion of δ-5-3 β-hydroxyl steroids to the δ-4-3-keto configuration and is involved in steroid hormone synthesis. It has been shown to be expressed in normal trophoblastic tissue and benign and neoplastic trophoblastic lesions. HSD3B1 has not been detected in a large number of lung, breast, and uterine carcinomas; however, its expression has not been studied in a wide variety of other nontrophoblastic neoplasms. To test if HSD3B1 is highly specific for normal trophoblasts and trophoblast-associated lesions, we examined the expression of HSD3B1 in a wide spectrum of tumors.

Methods: Tissue microarrays containing 473 carcinomas from the lung, breast, ovary, uterus, liver, pancreas, stomach, and colon; 32 ovarian granulose cell tumors; and 12 adrenocortical adenomas were studied by immunohistochemistry using a commercially available monoclonal antibody, HSD3B1. One tissue microarray containing normal tissues was also included. Positive staining of intermediate trophoblasts and syncytiotrophoblasts in normal placental tissue served as a positive control.

Results: Normal tissues and tumors from the various sites were all negative for HSD3B1 except for 2 adrenocortical adenomas with weak focal immunoreactivity.

Conclusions: Our study further confirmed that HSD3B1 is a highly specific trophoblast-associated marker that can be used in the distinction of trophoblastic tumorlike lesions and tumors from nontrophoblastic lesions and tumors.

*Department of Pathology and Laboratory Medicine, Shin-Kong Wu Ho-Su Memorial Hospital; †Department of Pathology, School of Medicine, College of Medicine, Taipei Medical University; and ‡Department of Pathology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan.

Address correspondence and reprint requests to Tsui-Lien Mao, MD, Department of Pathology, National Taiwan University Hospital and College of Medicine, National Taiwan University, 7 Chung-Shan South Rd, Taipei, Taiwan. E-mail: tlmao@ntu.edu.tw.

This study was supported by a grant from the NTUH (99-S1363 to T.-L.M.).

The authors declare no conflicts of interest.

Received October 19, 2012

Accepted November 18, 2012

Copyright © 2013 by IGCS and ESGO