Underexpression of 4 Placenta-Associated MicroRNAs in Complete Hydatidiform Moles

Na, Quan; Wang, Dan; Song, Weiwei MD

International Journal of Gynecological Cancer:
doi: 10.1097/IGC.0b013e3182574439
Gestational Trophoblastic Neoplasm
Abstract

Objective: Several placental microRNAs (miRNAs) have been identified as placenta-associated miRNAs with the potential of estimating the condition of the placenta. However, our understanding of these miRNAs is limited. The aim of this study was to determine the expression of 8 placenta-associated miRNAs (miR-512-3p, miR-517a, miR-517b, miR-518b, miR-519a, miR-1185, miR-1283, and miR-1323) in complete hydatidiform mole (CHM).

Methods: Samples were obtained from patients with CHM (CHM group, n = 12) and elective terminations of normal pregnancy (control group, n = 20). We detected differentially expressed placenta-associated miRNAs in placenta by quantitative real-time reverse transcriptase–polymerase chain reaction analysis. Subsequently, we assessed the expression location of differentially expressed miRNAs by in situ hybridization analysis.

Results: Four placenta-associated miRNAs (miR-517a, miR-517b, miR-518b, and miR-519a) were underexpressed in the CHM group, compared with the control group (P < 0.01). When further investigating these 4 miRNAs with regard to in vivo localization by in situ hybridization, we found that 2 miRNAs (miR-517b and miR-518b) were detected exclusively in the trophoblast layer, with little signal (if any) observed in villous stroma cells.

Conclusions: The results show that 4 miRNAs (miR-517a, miR-517b, miR-518b, and miR-519a) are deregulated in CHM, which suggests the involvement of these miRNAs in the functions of CHM placenta.

Author Information

Department of Obstetrics and Gynecology, The Affiliated Shengjing Hospital of China Medical University, Shenyang, People’s Republic of China.

Address correspondence and reprint requests to Weiwei Song, MD, Department of Obstetrics and Gynecology, The Affiliated Shengjing Hospital of China Medical University, Shenyang, Liaoning Province 110004, People’s Republic of China. E-mail: songww@sj-hospital.org.

This study was supported by the Natural Science Foundation of China (30672237).

The authors declare that they have no financial relationship with the organization that sponsored the research. The authors have had full control of all primary data.

Received January 10, 2012

Accepted March 26, 2012

Copyright © 2012 by IGCS and ESGO