Inflammatory Bowel Diseases

Skip Navigation LinksHome > June 2014 - Volume 20 - Issue 6 > Alterations in the Intestinal Microbiome (Dysbiosis) as a Pr...
Inflammatory Bowel Diseases:
doi: 10.1097/MIB.0000000000000036
Original Basic Science Articles

Alterations in the Intestinal Microbiome (Dysbiosis) as a Predictor of Relapse After Infliximab Withdrawal in Crohn's Disease

Rajca, Sylvie MD1; Grondin, Virginie BS1; Louis, Edouard MD, PhD2; Vernier-Massouille, Gwenola MD3; Grimaud, Jean-Charle MD, PhD4; Bouhnik, Yoram MD, PhD5; Laharie, David MD, PhD6; Dupas, Jean-Louis MD, PhD7; Pillant, Helene MD8; Picon, Laurence MD9; Veyrac, Michel MD10; Flamant, Mathurin MD, PhD11; Savoye, Guillaume PhD12; Jian, Raymond MD, PhD13; Devos, Martine MD, PhD14; Paintaud, Gilles MD, PhD15; Piver, Eric MD, PhD9; Allez, Matthieu MD, PhD16; Mary, Jean Yves PhD17; Sokol, Harry MD, PhD1; Colombel, Jean-Frederic MD, PhD18; Seksik, Philippe MD, PhD1

Supplemental Author Material
Collapse Box


Background: Crohn's disease (CD)–associated dysbiosis could predispose patients to relapse. Gut microbiota composition of patients from the prospective cohort study designed to identify predictive factors of clinical relapse after infliximab discontinuation (STORI Study) was investigated to determine the impact of dysbiosis in CD relapse.

Methods: Fecal samples from 33 patients with CD in this cohort were collected at baseline, 2 months, 6 months, and at the end of the follow-up period (19 relapsers and 14 nonrelapsers). Healthy volunteers subjects (n = 29) were used as a control group. The fecal microbiota composition was assessed using quantitative PCR, and comparisons between the patient groups were made at different time points using the Wilcoxon test. The analysis of the time-to-relapse was performed according to the baseline median level of each bacterial signal.

Results: Dysbiosis was observed in patients with CD compared with healthy subjects, and it was characterized by low mean counts of Firmicutes (Clostridium coccoides [P = 0.0003], C. leptum [P < 0.0001], and Faecalibacterium prausnitzii [P = 0.003]). Lower rates of Firmicutes were seen in relapsers compared with nonrelapsers. Moreover, a low rate of F. prausnitzii (P = 0.014) and a low rate of Bacteroides (P = 0.030) predicted relapse independently from high C reactive protein level (P = 0.0001).

Conclusions: In this work, we report that CD-associated dysbiosis, characterized by a decrease in Firmicutes, correlates with the time-to-relapse after infliximab withdrawal. A deficit in some bacterial groups or species, such as F. prausnitzii, may represent a predictive factor for relapse. Restoring normobiosis in CD could be a new goal for optimal CD management.

© Crohn's & Colitis Foundation of America, Inc.


Article Tools


Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.