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The Commensal Bacterium Faecalibacterium prausnitzii Is Protective in DNBS-induced Chronic Moderate and Severe Colitis Models

Martín, Rebeca PhD*,†,‡; Chain, Florian PhD*,†; Miquel, Sylvie PhD*,†; Lu, Jun; Gratadoux, Jean-Jacques*,†; Sokol, Harry MD, PhD*,†,§,‖; Verdu, Elena F. MD, PhD; Bercik, Premysl MD; Bermúdez-Humarán, Luis G. PhD*,†; Langella, Philippe PhD*,†,‡

Inflammatory Bowel Diseases:
doi: 10.1097/01.MIB.0000440815.76627.64
Original Basic Science Articles
Abstract

Background: The abundance of Faecalibacterium prausnitzii, an abundant and representative bacterium of Firmicutes phylum, has consistently been observed to be lower in patients with Crohn's disease than in healthy individuals. We have shown that both F. prausnitzii and its culture supernatant (SN) have anti-inflammatory and protective effects in a TNBS-induced acute colitis mouse model. Here, we tested the effects of both F. prausnitzii and its SN in moderate and severe DNBS-induced chronic colitis mouse models.

Methods: Colitis was induced by intrarectal administration of DNBS. After either 4 or 10 days of recovery (severe and moderate protocols, respectively), groups of mice were intragastrically administered either with F. prausnitzii A2-165 or with its culture SN for 7 or 10 days. Three days before being sacrificed, colitis was reactivated by administration of a lower dose of DNBS. The severity of colitis at the time of being sacrificed was assessed by weight loss and macroscopic and microscopic scores. Myeloperoxidase (MPO) activity, cytokine levels, lymphocyte populations, and changes in microbiota were studied.

Results: Intragastric administration of either F. prausnitzii or its SN led to a significant decrease in colitis severity in both severe and moderate chronic colitis models. The lower severity of colitis was associated with down-regulation of MPO, pro-inflammatory cytokines, and T-cell levels.

Conclusions: We show, for the first time, protective effects of both F. prausnitzii and its SN during both the period of recovery from chronic colitis and colitis reactivation. These results provide further evidence that F. prausnitzii is an anti-inflammatory bacterium with therapeutic potential for patients with inflammatory bowel disease.

In Brief

Article first published online 10 January 2014

Author Information

*INRA, Commensal and Probiotics-Host Interactions Laboratory, UMR 1319 Micalis, Jouy-en-Josas, France;

AgroParisTech, UMR 1319 Micalis, Jouy-en-Josas, France;

Farncombe Family Digestive Health Research Institute, Faculty of Health Sciences, McMaster University, Hamilton, Ontario, Canada;

§Department of Gastroenterology and Nutrition, AP-HP, Hôpital Saint-Antoine and UPMC University of Paris, Paris, France; and

Equipe AVENIR Gut Microbiota and Immunity, INSERM U1057/UMR CNRS 7203, Université Pierre et Marie Curie 6, Paris, France.

Reprints: Philippe Langella, PhD, Head of Commensals and Probiotics-Host Interactions Laboratory, MICALIS Institute, INRA, Domaine de Vilvert, 78352 Jouy en Josas, France (e-mail: philippe.langella@jouy.inra.fr).

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Web site (www.ibdjournal.org).

The authors have no conflicts of interest to disclose.

Received October 16, 2013

Accepted November 21, 2013

© Crohn's & Colitis Foundation of America, Inc.

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