Institutional members access full text with Ovid®

Share this article on:

Increased Activation of Latent TGF-β1 by αVβ3 in Human Crohn's Disease and Fibrosis in TNBS Colitis Can Be Prevented by Cilengitide

Li, Chao MD*; Flynn, Robert S. MD*; Grider, John R. PhD*,†; Murthy, Karnam S. PhD*,†; Kellum, John M. MD; Akbari, Homayoon MD, PhD; Kuemmerle, John F. MD*,†

doi: 10.1097/MIB.0b013e3182a8452e
Original Basic Science Articles

Background: Strictures develop in >30% of patients affected with Crohn's disease. No available medication prevents stricture development in susceptible patients. In Crohn's strictures, but not adjacent normal intestine, TGF-β1 increases in muscularis smooth muscle, increasing collagen I production and strictures. Muscle cells express αVβ3 integrin containing an Arg-Gly-Asp (RGD) binding domain. The aim was to determine whether increased TGF-β1 levels in strictures were the result of latent TGF-β1, which contains an RGD sequence, binding to and activation by αVβ3; and whether cilengitide, which is an RGD-containing αVβ3 integrin inhibitor, decreases TGF-β1 activation and development of fibrosis in chronic 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis.

Design: Muscle cells isolated from Crohn's disease strictures and normal resection margin and from the colon of rats after 42 days of chronic TNBS-induced colitis were used to prepare RNA and protein lysates and to initiate primary cultures. The mechanisms leading to increased TGF-β1 activation, collagen I production, and fibrosis were examined in human muscle and in rats. Human cultured cells in vitro and rats in vivo were treated with cilengitide to determines it efficacy to decrease TGF-β1-activation, collagen production, and decrease the development of fibrosis.

Results: Latent TGF-β1 is activated by the αVβ3 RGD domain in human and rat intestinal smooth muscles. Increased activation of TGF-β1 in Crohn's disease and in TNBS-induced colitis causes increased collagen production, and fibrosis that could be inhibited by cilengitide.

Conclusions: Cilengitide, an αVβ3 integrin RGD inhibitor, could be a novel treatment to diminish excess TGF-β1 activation, collagen I production, and development of fibrosis in Crohn's disease.

Article first published online 18 September 2013

Departments of *Medicine,

Physiology and Biophysics, and

Surgery, VCU Program in Enteric Neuromuscular Sciences, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA.

Reprints: John F. Kuemmerle, MD, VCU Program in Enteric Neuromuscular Sciences, Medical College of Virginia Campus, Virginia Commonwealth University, Molecular Medicine Research Building 5-036, PO Box 980341, Richmond, VA 23298-0341 (e-mail: john.kuemmerle@vcu.edu).

Supported by National Institutes of Health: National Institutes for Diabetes, Digestive and Kidney Diseases Grant DK49691 (to J. F. Kuemmerle).

The authors have no conflicts of interest to disclose.

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Web site (www.ibdjournal.org).

Received July 08, 2013

Accepted August 06, 2013

© Crohn's & Colitis Foundation of America, Inc.
You currently do not have access to this article

To access this article:

Note: If your society membership provides full-access, you may need to login on your society website