Skip Navigation LinksHome > April 2013 - Volume 19 - Issue 5 > Colonoscopy and µPET/CT are Valid Techniques to Monitor Infl...
Inflammatory Bowel Diseases:
doi: 10.1097/MIB.0b013e3182802c7c
Original Basic Science Articles

Colonoscopy and µPET/CT are Valid Techniques to Monitor Inflammation in the Adoptive Transfer Colitis Model in Mice

Heylen, Marthe MPharm*; Deleye, Steven MSc; De Man, Joris G. MSc*; Ruyssers, Nathalie E. MSc, PhD*; Vermeulen, Wim MSc, PhD*; Stroobants, Sigrid MD, PhD†,‡; Pelckmans, Paul A. MD, PhD*,§; Moreels, Tom G. MD, PhD*,§; Staelens, Steven MSc, PhD; De Winter, Benedicte Y. MD, PhD*

Collapse Box

Abstract

Background: Preclinical in vivo research on inflammatory bowel diseases requires proper animal models and techniques allowing longitudinal monitoring of colonic inflammation without the need to kill animals. We evaluated colonoscopy and μ-positron emission tomography/computed tomography (μPET/CT) as monitoring tools in a model for chronic colitis in mice.

Methods: Colitis was induced by adoptive transfer of CD4+CD25CD62L+ T cells in immunocompromised severe combined immunodeficient mice. Three study protocols were designed. In study 1, colonoscopy and µPET/CT were performed once, 4 weeks after transfer. In study 2 and study 3, colitis was sequentially followed up through colonoscopy (study 2) or colonoscopy plus µPET/CT (study 3). Each study included postmortem evaluation of colonic inflammation (macroscopy, microscopy, and myeloperoxidase activity).

Results: In study 1, both colonoscopy and µPET/CT detected colitis 4 weeks after transfer. Study 2 showed a gradual increase in colonoscopic score from week 2 (1.4 ± 0.6) to week 8 (6.0 ± 1.1). In study 3, colitis was detected 2 weeks after transfer by µPET/CT (2.0 ± 0.4) but not by colonoscopy, whereas both techniques detected inflammation 4 and 6 weeks after transfer. Colonoscopy correlated with µPET/CT (r = 0.812, 0.884, and 0.781, respectively) and with postmortem analyses in all 3 studies.

Conclusions: Adoptive transfer of CD4+CD25CD62L+ T cells in severe combined immunodeficient mice results in a moderate chronic colitis. Evolution of colitis could be monitored over time by both colonoscopy and µPET/CT. µPET/CT seems to detect inflammation at an earlier time point than colonoscopy. Both techniques represent reliable and safe methods without the need to kill animals.

© Crohn's & Colitis Foundation of America, Inc.

You currently do not have access to this article.

You may need to:

Note: If your society membership provides for full-access to this article, you may need to login on your society’s web site first.

Login

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.