Skip Navigation LinksHome > February 2013 - Volume 19 - Issue 2 > Fecal MMP-9: A New Noninvasive Differential Diagnostic and...
Inflammatory Bowel Diseases:
doi: 10.1002/ibd.22996
Original Clinical Articles

Fecal MMP-9: A New Noninvasive Differential Diagnostic and Activity Marker in Ulcerative Colitis

Annaházi, Anita MD*,†; Molnár, Tamás MD, PhD*; Farkas, Klaudia MD, PhD*; Rosztóczy, András MD, PhD*; Izbéki, Ferenc MD, PhD*; Gecse, Krisztina MD, PhD*; Inczefi, Orsolya MD*; Nagy, Ferenc MD, PhD*; Földesi, Imre PhD*; Szűcs, Mónika MSc; Dabek, Marta PhD; Ferrier, Laurent PhD; Theodorou, Vassilia PhD; Bueno, Lionel PhD; Wittmann, Tibor MD, PhD*; Róka, Richárd MD, PhD*

Collapse Box

Abstract

Background: Ulcerative colitis (UC) is characterized by frequent relapses, with the presence of colorectal inflammation and mucosal lesions. Matrix-metalloprotease 9 (MMP-9) is elevated in colonic biopsies, urine, and blood plasma of UC patients. MMP-9 has been suggested as a predictor of UC in the urine of children; however, 20% of the controls tested positive. So far, fecal MMP-9 levels have never been measured. Our aims were: 1) to compare fecal MMP-9 levels in UC patients to control subjects and a functional gastrointestinal disorder characterized by diarrhea (IBS-D); 2) to test the correlation between UC disease activity and fecal levels of MMP-9; and 3) to correlate fecal MMP-9 levels with a known fecal marker of UC activity, calprotectin.

Methods: UC (n = 47), IBS-D (n = 23) patients, and control subjects (n = 24) provided fecal samples for MMP-9 analysis. In UC patients, disease severity was evaluated by the Mayo score. Fecal MMP-9 and calprotectin levels were measured by enzyme-linked immunosorbent assay and lateral flow assay, respectively.

Results: MMP-9 was undetectable or ≤0.22 ng/mL in the feces of all controls and IBS-D patients. In UC patients, fecal MMP-9 levels significantly correlated with the overall Mayo score (P < 0.001), the endoscopic score (P < 0.001), and the serum C-reactive protein levels (P = 0.002). Additionally, in UC patients fecal MMP-9 levels showed a significant correlation with a known disease activity marker, fecal calprotectin (P = 0.014).

Conclusions: These results highlight fecal MMP-9 as a useful tool in the differential diagnosis of diarrheic disorders and in the noninvasive evaluation of disease activity and mucosal healing in UC.

© Crohn's & Colitis Foundation of America, Inc.

You currently do not have access to this article.

You may need to:

Note: If your society membership provides for full-access to this article, you may need to login on your society’s web site first.

Login

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.