Inflammatory Bowel Diseases

Skip Navigation LinksHome > January 2013 - Volume 19 - Issue 1 > Quantitative Phenotyping of Inflammatory Bowel Disease in th...
Inflammatory Bowel Diseases:
doi: 10.1002/ibd.23006
Original Basic Science Articles

Quantitative Phenotyping of Inflammatory Bowel Disease in the IL-10-deficient Mouse by Use of Noninvasive Magnetic Resonance Imaging

Michael, Svenja*; Keubler, Lydia M. DVM, PhD*; Smoczek, Anna*; Meier, Martin Dr.rer.nat.*; Gunzer, Florian; Pöhlmann, Christoph; Krause-Buchholz, Udo Dr.rer.nat.§; Hedrich, Hans-J. DVM*; Bleich, André DVM, PhD*

Supplemental Author Material
Collapse Box


Background: Although magnetic resonance imaging (MRI) is an increasingly used diagnostic tool in the assessment of inflammatory bowel disease (IBD) in humans, diagnosis and quantitation of intestinal inflammation in animal models of IBD still depends on ex vivo techniques. The aim of this study was to evaluate whether high-field MRI is suitable for the quantitative phenotyping of gut inflammation in a dextran sulfate sodium (DSS)-triggered interleukin (IL)10-deficient (IL-10−/−) mouse model of IBD, especially in longitudinal studies.

Methods: Using colitis-susceptible and -resistant backgrounds, MRI and ex vivo analyses were applied to characterize this specific model, differentiating disease severity and time-dependent alterations. Colon wall thickness, cecum wall tissue intensity, spleen, and mesenteric lymph node (MLN) volumes were evaluated 1, 2, 4, and 12 weeks after disease onset by T2-weighted MRI. Ex vivo parameters included histology, spleen, and MLN weight and analysis of cytokine expression.

Results: MRI and ex vivo determined parameters correlated well, revealing a mouse strain-specific colitis development over time with characteristics typical for the DSS model in the initial and for the IL-10−/− model in the chronic phase. To evaluate the use of high-field MRI for monitoring therapeutic studies, mice with a profound colitis were treated with IL-10-producing Saccharomyces boulardii and monitored by MRI.

Conclusions: MRI can be utilized to quantify colitis development in the IL-10−/− model of IBD. Therefore, this noninvasive technique might be highly advantageous for an individual follow-up of colitis development in chronic models of IBD, facilitating the reduction of animal numbers in this kind of research.

© Crohn's & Colitis Foundation of America, Inc.

You currently do not have access to this article.

You may need to:

Note: If your society membership provides for full-access to this article, you may need to login on your society’s web site first.


Article Tools


Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.