Skip Navigation LinksHome > August 1996 - Volume 88 - Issue 2 > Determination of Fetal RhD Status From Uncultured Amniocytes...

ACOG MEMBER SUBSCRIPTION ACCESS

If you are an ACOG Fellow and have not logged in or registered to Obstetrics & Gynecology, please follow these step-by-step instructions to access journal content with your member subscription.

Obstetrics & Gynecology:
Articles: PDF Only

Determination of Fetal RhD Status From Uncultured Amniocytes.

DILDY, GARY A. MD; JACKSON, G. MARC MD; WARD, KENNETH MD

Collapse Box

Abstract

Objective: To determine the accuracy of DNA analysis for fetal RhD status using polymerase chain reaction (PCR) on uncultured amniocytes.

Methods: Three hundred forty-seven amniotic fluid (AF) samples obtained for a variety of clinical indications were tested for fetal RhD status using PCR and primers specific for the genes coding for D and Cc/Ee. Fetal RhD status was confirmed by serologic methods on neonatal cord blood obtained at the time of delivery.

Results: In 346 of 347 cases (99.7%), the PCR results of AF were in agreement with the serologic RhD blood type. The single error was in an RhD-negative fetus (misidentified as RhD-positive) whose AF was contaminated with blood from an RhD-positive mother. The likelihood of an erroneous result was 0.3%.

Conclusion: Polymerase chain reaction-based analysis of fetal RhD status using uncultured amniocytes and established primers appears to be highly accurate.

(C) 1996 The American College of Obstetricians and Gynecologists

Login

ACOG MEMBER SUBSCRIPTION ACCESS

If you are an ACOG Fellow and have not logged in or registered to Obstetrics & Gynecology, please follow these step-by-step instructions to access journal content with your member subscription.

Article Tools

Share

Article Level Metrics