Sexual Assault Nurse Examiners (SANEs) were introduced in the United Kingdom in 2001, but there is a lack of knowledge about their role and the services they provide. The aim of this study was to explore the role of SANEs currently working in England. Qualitative semistructured interviews explored the training experiences, roles, and working practices of five SANEs in a variety of settings. The findings identified three categories: training and qualifications, SANE care, and working within multidisciplinary teams (MDT). SANEs have complex roles that involve patient care as well as collection of forensic evidence. There was variation in service provision and training of SANEs interviewed and differences in how they felt their role was regarded by members of the MDT. The findings suggest that SANE services need further evaluation to determine a model of practice that can be consistently implemented to provide both optimal patient care and reliable forensic evidence.