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Determination of amino acids in urine of patients with prostate cancer and benign prostate growth

Sroka, Wiktor D.; Boughton, Berin A.; Reddy, Priyanka; Roessner, Ute; Słupski, Piotr; Jarzemski, Piotr; Dąbrowska, Anita; Markuszewski, Michał J.; Marszałł, Michał P.

European Journal of Cancer Prevention: March 2017 - Volume 26 - Issue 2 - p 131–134
doi: 10.1097/CEJ.0000000000000248
Short Paper: Urological Cancers

Prostate cancer is the leading type of cancer diagnosed in men. Serum prostate-specific antigen levels and digital rectal exam are far from perfect when it comes to differentiation of patients with prostate cancer and benign prostatic hyperplasia. In this study, we attempt to determine whether amino acids can be used as prostate cancer biomarkers. Concentrations of derivatized amino acids and amines were quantified by liquid chromatography tandem mass spectrometry. A total of 100 urine samples from the two groups including samples provided before and after prostate massage were examined quantitatively for amino acid and amine concentrations with 50 urine samples collected from cancer patients and 50 samples from patients diagnosed with benign prostatic hyperplasia. Arginine, homoserine, and proline were more abundant in urine samples of cancer patients compared with arginine, homoserine, and proline levels determined in urine collected from patients with benign growth. We also show that sarcosine is not a definitive indicator of prostate cancer when analyzed in urine samples collected either before or after prostate massage.

Departments of aMedicinal Chemistry

bTheoretical Foundations of Biomedical Sciences and Medical Informatics

cDepartment of Urology, Jan Biziel Hospital, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun

dDepartment of Biopharmaceutics and Pharmacodynamics, Medical University of Gdansk, Gdansk, Poland

eMetabolomics Australia, School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia

Correspondence to Michał P. Marszałł, PhD, Department of Medicinal Chemistry, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, ul. Jurasza 2, 85-094 Bydgoszcz, Poland Tel: +48 52 585 3540; fax: +48 52 585 3804; e-mail: mmars@cm.umk.pl

Received September 15, 2015

Accepted February 7, 2016

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