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Epidemiology:
doi: 10.1097/01.ede.0000362440.12710.bf
Abstracts: ISEE 21st Annual Conference, Dublin, Ireland, August 25-29, 2009: Symposium Abstracts

Effects of Exposure to Metal-Rich Air Particles on DNA Methylation

Tarantini, Letizia*; Hou, Lifang†; Bonzini, Matteo‡; Apostoli, Pietro§; Pegoraro, Valeria*; Schwartz, Joel¶; Bertazzi, Pier Alberto*; Baccarelli, Andrea*

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*University of Milan and IRCCS Maggiore Hospital Foundation, Milan, Italy; †Northwestern University, Chigago, IL, United States; ‡Department of Clinical and Biological Sciences, University of Insubria, Varese, Italy; §University of Brescia, Brescia, Italy; and ¶Harvard School of Public Health, Boston, MA, United States.

ISEE-0225

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Background and Objective:

Air particles and their metal components induce aberrant gene expression related to their systemic effects, including cardiorespiratory disease and lung cancer. The mechanisms determining particle-related gene deregulation are largely undetermined. In foundry workers with well-characterized exposure to airborne metal-rich particles, we evaluated promoter DNA methylation, a major epigenetic mechanism for expression inhibition, in ten cardiorespiratory and cancer genes.

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Methods:

We measured DNA methylation (expressed as %5-methylcytosine) by PCR-Pyrosequencing on blood DNA from 63 foundry workers on the first day of a workweek (following two days off) and after three days of work (post-exposure). Personal exposures to particulate matter with diameter <10 μm (PM10) or <1 μm (PM1), and their metal components (Cr, Cd, As, Pb, Mn, Ni) were estimated using area-specific measurements.

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Results:

Post-exposure samples exhibited a significant increase in methylation of APC (difference = 0.24, P = 0.005) and p16 (difference = 0.14, P = 0.006), and a decrease in Rassf1A (difference = −1.09; P < 0.001), CDH13 (difference = −0.61; P = 0.001), TNFα (difference = −0.35; P = 0.005), and IFNγ (difference = −0.86; P < 0.001). No differences were found in p53, Il-6, eNOS, and Et1.

In post-exposure samples, APC methylation was associated with PM10 (β = 0.65; P < 0.001) and PM1 (β = 0.75; P = 0.002) exposure; Rassf1A methylation with Cr (β = −2.59; P = 0.03), Pb (β = −3.44; P = 0.005), and Cd (β = −2.68; P = 0.01); CDH13 methylation with Cd (β = −2.90; P = 0.02). TNFα methylation was borderline associated with Ni (β = −1.65; P = 0.07) and Mn (β = -0.65; P = 0.06). IFNγ methylation did not show any consistent association with exposure levels.

Some of the exposures were associated with methylation in both pre- and post-exposure samples, possibly reflecting effects operating over an extended timeframe. Et1 methylation was associated with Pb (β = −3.03; P = 0.005) and Cd (β = −2.46; P = 0.01), eNOS with PM10 (β-0.71; P = 0.03), Cr (β = −1.37; P = 0.01), Pb (β = −1.20; P = 0.04) and Cd (β = -1.41 P = 0.004).

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Conclusions:

Metal-rich air particles modify the DNA methylation of genes related to cardiorespiratory and cancer outcomes.

Cited By:

This article has been cited 1 time(s).

Environmental Health
A panel study of occupational exposure to fine particulate matter and changes in DNA methylation over a single workday and years worked in boilermaker welders
Kile, ML; Fang, SN; Baccarelli, AA; Tarantini, L; Cavallari, J; Christiani, DC
Environmental Health, 12(): -.
ARTN 47
CrossRef
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© 2009 Lippincott Williams & Wilkins, Inc.

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