The Value of Risk-Factor (“Black-Box”) Epidemiology
Greenland, Sander*; Gago-Dominguez, Manuela†; Castelao, Jose Esteban†
From the *Departments of Epidemiology and Statistics, University of California Los Angeles, Los Angeles, California; and the †Department of Preventive Medicine, USC/Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, Los Angeles, California.
Editors’ note: Commentaries on this article appear on pages 519, 521, 523, and 525, and a response from the author on page 527.
Submitted 2 April 2003; final version accepted 21 May 2004.
Correspondence: Sander Greenland, Departments of Epidemiology and Statistics, University of California Los Angeles, Los Angeles, CA 90095-1772. E-mail: firstname.lastname@example.org
Risk-factor epidemiology has been denigrated by some as an empty search for associations, unguided by underlying theory. It has been defended for occasionally identifying useful (if poorly understood) potential interventions. We further defend risk-factor epidemiology as a valuable source of seemingly unrelated facts that await coherent explanation by novel theories and that provide empiric tests of theories. We illustrate these points with a theory that invokes lipid peroxidation as an explanation of an apparently incoherent accumulation of facts about renal-cell carcinoma.1 The example illustrates the value of viewing epidemiologic, laboratory, and clinical observations as a body of facts demanding explanation by proposed causal theories, whether or not those observations were collected with any hypothesis in mind.
Most epidemiologic articles are built around a standard format in which the design, execution, and data from the study are described in some detail, along with statistical analyses to identify “risk factors” (antecedents of adverse health outcomes that remain associated with the outcomes after adjustments for measured potential confounders). With rare exception, these analyses account only for the possible role of random error (“chance”) in producing the results; possible roles for biases and causal mechanisms are dealt with by informal discussion.
This standard format has been criticized for its failure to account adequately for biases and their interactions (apart from adjustments for measured confounders); the alternative offered is sensitivity analysis and its Monte-Carlo and Bayesian extensions.2–8 The standard format has also been attacked for its failure to adequately justify the study within a context of biomedical or social theory.9–11 Some of the latter criticisms have gone so far as to imply that much of epidemiology is pointless and wasteful “black-box” research that yields too many nonsense associations and false alarms.12
Among defenses of “black-box” epidemiology are that it has identified useful interventions and that the absence of a known causal mechanism has no bearing on the validity of the study results.13 Skrabanek,12 however, responded that the latter point was never in dispute; he asserted instead that “there is no logical link between black box epidemiologic studies and science” and that risk-factor epidemiology “cannot contradict pertinent scientific data.” We argue here that such claims are logically erroneous and counterproductive.
One genuine problem of risk-factor epidemiology is overinterpretation of observed associations as causal. We believe that this problem stems from pressure on researchers to demonstrate scientific or policy relevance of their results, or at least to explain their results as arising from something other than error or bias. We argue that it is a mistake to impose such an explanatory burden on empiric research reports, and that the problem of overinterpretation could be addressed by encouraging a descriptive approach to risk-factor studies. We then illustrate our arguments with a case study on the epidemiology of renal cell carcinoma.1
THE VALUE OF DESCRIPTIVE OBSERVATION REPORTS
A theory could be said to explain an observation (or fact) when the observation can be deduced from or is predicted by the theory. According to one popular philosophy of science, the primary role of scientific observation is to supply the facts that require explanation by theories. From this viewpoint, assertions that risk-factor studies could “provide testable hypotheses of causality”12 or are “hypothesis-generating” are logically backward, even though they could reflect some of the psychology of theory creation.14 Furthermore, purely descriptive (atheoretical) approaches to the reporting of single epidemiologic studies (and even metaanalyses) are not only scientific, but beneficial; the publication of mere observations is useful, because such observations supply data for the scientific community to use in tests of theories, including theories not even conceived at the time of publication. This means that the incoherence or implausibility of observations in light of current theory should never be a deterrent to publication; a field that appears to be nothing more than an incoherent jumble of haphazard or implausible observations could be rapidly transformed by the introduction of a new and unifying theory.
One consequence of this philosophy is that (contrary to some teachings) precision and replication are not secondary in importance to validity and novelty in study design. Precision and replication establish the facts that need to be explained by theories. A study that is perfectly valid but so imprecise as to be incapable of determining the direction and size of an association is of little or no use in testing predictions about the association. Precise null results are much more informative than so-called “null” or “nonsignificant” findings that are compatible with a wide range of possibilities (and hence are of little use for testing theories). Thus, precision, not statistical significance, measures the statistical importance of results.15 Although many imprecise studies could be combined to provide enough data to precisely establish an association's size, this phenomenon involves some form of study replication.
Without sufficiently precise data on variation in disease occurrence, there is little that epidemiology can contribute to choosing among proposed social or biologic theories of the disease. With enough description, however, researchers have on hand immediate tests of predictions from proposed theories. A large bank of observations (epidemiologic, laboratory, and clinical) allows rapid winnowing down of theories to promising candidates. The observations can be used regardless of whether they were gathered for the purpose of testing a given theory, although observations collected for that purpose might yield more powerful tests of that theory than would other data sources.
Selecting or formulating theories based on the proportion of observations explained raises the specter of overfitting, that is, proposing a theory so flexible that it can explain not only all that has been observed, but also almost anything likely to be observed. Parsimony (keeping the theory simple) is commonly offered as a safeguard against overfitting.14 Nonetheless, it has been argued that uncritical devotion to parsimony can be harmful when modeling epidemiologic data-generating processes and methodologic problems, because parsimony in statistical models often leads to overconfident epidemiologic inferences.8,16 A more direct safeguard against overfitting is to check whether a proposed theory excludes some plausible possibilities for data that were not used in formulating the theory (such as observation not yet made). This safeguard is the well-known falsifiability or testability criterion for preferring theories.
A theory formulated to explain laboratory results could have a large body of epidemiologic associations available for testing purposes, provided epidemiologists have been thorough in examining and reporting their data. More generally, a promising biologic theory will provide a link among observations from various research specialties (eg, epidemiologic, experimental, and clinical), as well as links among different risk factors; such a theory will also make predictions that are testable with further feasible observations.
THE EPIDEMIOLOGY OF RENAL CELL CARCINOMA
As an example of these points, the epidemiology of renal cell carcinoma might seem an incoherent body of facts, but for a theory that explains both epidemiologic and laboratory results. That theory posits lipid peroxidation as an intermediate step that leads to a final common pathway shared by numerous observed risk factors such as obesity, hypertension, smoking, oophorectomy, hysterectomy, parity, diabetes, and dietary antioxidants.1 The theory thus explains the epidemiology of renal cell carcinoma; as we discuss, it also explains numerous experimental and clinical findings, and has implications for disease prevention.
We begin by summarizing the literature on risk factors for renal cell carcinoma. This summary is analogous to the data-description portion of a study report; it is a dry description of our perception of published data. For brevity, we rely heavily on reviews of renal cell carcinoma, along with some original reports, and provide only rough summaries of previous findings. Like with data descriptions in study reports, the reviews and our summary are subject to reporting errors and biases (eg, publication bias). Unlike primary data descriptions, however, the reader can check the following summaries against the literature:
1. Age: Renal cell carcinoma rates rise rapidly with age.17
2. Sex: Men have age-specific renal-cell carcinoma rates about double the rates in women.17
The remaining summaries refer to associations adjusted for age, sex, and (in some analyses) other variables:
1. Ethnicity: Renal cell carcinoma rates from U.S. cancer registry data are higher in blacks than in whites.18
2. Obesity: Several case-control and cohort studies have reported obesity associated with renal cell carcinoma.17 For example, body mass index (BMI, defined as kg/m2) over 30 appears associated with 4 times the renal cell carcinoma risk of BMI under 22.19
3. Hypertension: Several case-control and cohort studies have reported hypertension associated with renal cell carcinoma.17 A history of hypertension appears associated with a doubling of renal cell carcinoma risk.19
4. Smoking: Several case-control studies have reported cigarette use associated with renal cell carcinoma.17 For example, smokers appear to have a 40% higher risk of renal cell carcinoma than nonsmokers.20
5. Oophorectomy and hysterectomy: Although earlier case-control studies had reported no association between hysterectomy or oophorectomy and renal cell carcinoma, more recent investigations have observed these procedures associated with a doubling of renal cell carcinoma risk.21–24
6. Parity: Although previous case-control studies have reported no clear relation between parity and renal-cell carcinoma risk, more recent studies have reported positive associations.21,23–26 For example, women with 5 or more births appear to have twice the risk among women with one or 2 births.23–25
7. Antioxidants: In several case-control studies, high measured dietary intake of antioxidants was associated with a 20% to 50% decrease in the risk of renal cell carcinoma relative to low intakes.27,28
8. Diabetes: Although some case-control studies had reported no association between diabetes and renal cell carcinoma, more recent investigations have consistently found positive associations.21 Several large case-control and cohort studies reported diabetes associated with a 30% to 70% elevation in renal cell carcinoma risk.29–31
9. Preeclampsia: There have been case reports of renal cell carcinoma in women with severe preeclampsia or hypertension during pregnancy.32,33
10. Analgesics: Although results are not entirely consistent, several case-control studies have reported chronic use of analgesics (aspirin, other nonsteroidal antiinflammatory drugs, acetaminophen, and phenacetin) associated with an increased renal cell carcinoma risk.21,34
EXPERIMENTAL AND CLINICAL STUDIES OF LIPID PEROXIDATION
What sense can be made of a dozen risk factors ranging from hypertension to hysterectomy? This diversity might suggest that the associations arise from a variety of biologic mechanisms and methodologic problems; the associations might instead be dismissed as the sort of scrap heap that unchecked risk-factor epidemiology produces. A third alternative, however, is that there is a terminal pathway in renal cell carcinoma development affected by all the factors.
Rodent experiments point to lipid peroxidation of the proximal renal tubules as a principal mechanism in renal carcinogenesis.1 Renal tumors induced by ferric nitrilotriacetate in rodents appear to be the counterpart of human renal cell carcinoma.35 Similar to human renal cell carcinoma, rodent renal cell carcinoma incidence is higher in males than females, with histopathologic features marked by clear or granular-type cells.35,36 On the clinical side, lipid peroxidation markers are elevated in human renal cell carcinoma tissue.37,38
Thus, our next step is to summarize experimental and clinical observations that link the dozen epidemiologic risk factors discussed here to the hypothesized shared intermediate steps (lipid peroxidation) and the outcome (renal cell carcinoma):
1. Lipid peroxidation in serum increases with age in humans39 and in renal tissue increases with age in animals.40
2. Men have higher serum and plasma levels of lipid peroxidation than women.41–44 Testosterone treatment or oophorectomy increase ferric nitrilotriacetate-induced lipid peroxidation and renal cell carcinoma incidence in rodents, whereas estradiol treatment or castration decrease ferric nitrilotriacetate-induced lipid peroxidation and renal cell carcinoma incidence.36,45
3. Among patients with diabetes, African-Caribbeans appear to have higher lipid peroxidation levels than whites46 (we are unaware of relevant data for nondiabetics). Black have a higher prevalence of several conditions associated with elevated lipid peroxidation such as hypertension (in both men and women) and obesity (in women).47
4. Obesity/hypertension induces lipid peroxidation and the development of renal damage in rats.48 Obesity has been associated with elevated lipid peroxidation among human subjects, and the elevation appears to be removable by weight reduction.49–54
5. Hypertension has been associated with elevated lipid peroxidation among human subjects, and the elevation appears to be removable by antihypertensive therapy.55–57
6. Oophorectomy in rodents increases the extent of ferric nitrilotriacetate-induced lipid peroxidation of the renal tubules45 and the incidence of renal cell carcinoma.36 Female mice lacking both ovaries show increased lipid peroxide in serum.41 Human oophorectomy is also associated with elevated serum lipid peroxidation41; because human hysterectomy is usually accompanied by oophorectomy, we would expect hysterectomy to be related to lipid peroxidation as well.
7. Dietary antioxidants and antilipidemic agents prevent ferric nitrilotriacetate-induced lipid peroxidation, nephrotoxicity, or renal cancer development in rats,58–60 and they appear to inhibit lipid peroxidation in humans.61–64
8. Relevant to parity, pregnant rats have higher serum lipid peroxidation than nonpregnant rats,65 and pregnant women have higher lipid peroxidation than nonpregnant women.66
9. Tobacco smoking is associated with lipid peroxidation in humans.67,68
10. Diabetes has been associated with elevated lipid peroxidation.69
11. Preeclampsia is associated with increased lipid peroxidation in women.66,70,71
12. Aspirin, naproxen, and diclofenac appear to increase lipid peroxidation in humans and rodents.72–74 Diclofenac-induced nephrotoxicity in mice has been linked to its ability to increase lipid peroxidation.72
Regarding genetic factors, somatic mutations of the VHL gene seem to occur at high frequency in sporadic cases of renal cell carcinoma. It has been shown that renal carcinoma cells lacking a functional VHL gene express hypoxia-inducible genes such as VEGF75–77; furthermore, reintroduction of the wild-type VHL gene into these cells is sufficient to repress VEGF gene expression under normoxic conditions and to restore its normal regulation by hypoxia. It appears that hypoxia can induce lipid peroxidation,78 and also that lipid peroxidation can increase the expression of hypoxia-inducible genes.79
Not every study supports the lipid peroxidation–renal cell carcinoma hypothesis. Isolated normal renal tubular tissues have displayed higher levels of malondialdehyde (MDA; a lipid peroxidation marker) than have renal cell carcinoma tissues.80 4-hydroxy-2-nonenal (HNE) (another lipid peroxidation marker) protein adducts occur in kidneys in both normal and tumor cells, and immunomorphologic analyses suggest less HNE protein adducts in tumor than in normal cells.81 Although most studies have found increased lipid peroxidation in men relative to women,41–44 one study did not.82 In apparent conflict with point 5 above, some studies have suggested an association of diuretics or other antihypertensives with renal cell carcinoma21; others have reported no association.19,83 These results reflect uncertainties in separating the effect of hypertension from that of its medical treatment. One explanation is that antihypertensives increase risk directly while indirectly lowering risk through hypertension reduction. Indeed, one study observed blood pressure reduction associated with reduced renal cell carcinoma risk.84
Many of these associations (such as those with sex, smoking, and antioxidants) are explainable through other mechanisms or are not specific to kidney cancer. Nonetheless, these observations do not conflict with the lipid peroxidation hypothesis because the hypothesis does not preclude other mechanisms, and lipid peroxidation could play a role in other cancers. If and when a truly competing hypothesis is proposed, the challenge for its proponents will be to explain as large a proportion of the available evidence as does the lipid peroxidation hypothesis.
A promising theory will not only make predictions testable with further observations; it will also point to new observations that will make past observations useful in testing the theory. For example, antilipidemic agents appear to prevent lipid peroxidation in humans.62–64 In isolation, this observation says nothing about the lipid peroxidation hypothesis, but it can form part of another test of the theory when combined with data on the relation of antilipidemic agents to renal cell carcinoma. The limited available data support an inverse association of statins with cancers (including renal cell carcinoma),85 and further data could be easily collected in case-control studies of renal cell carcinoma. Lipid-lowering agents could exert beneficial effects in patients with different types of renal disease86 such as end-stage renal disease,87,88 a condition itself associated with an increased renal cell carcinoma risk89; thus, we would expect that further epidemiologic renal cell carcinoma data will support the lipid peroxidation hypothesis.
In summary, it appears that most available evidence can be explained by the lipid peroxidation hypothesis, and the amount of contradicting evidence is no more than what one might expect given the many sources of error and bias in typical studies. Therefore, we think that the lipid peroxidation hypothesis is well-supported at this time.
Research articles can be useful even if they only report how the study was done and what associations were observed without attempting to interpret or explain these observations in terms of methodologic, biologic, or social theories. Readers can weave the observations into their own explanatory theory, one that incorporates their knowledge of related literature. This is not to discourage the study authors from proposing their own explanations. In particular, the study authors are usually most familiar with the study methods and therefore often best equipped to offer methodologic explanations of their results (ie, explanations of those results based on the particulars of the study design and conduct rather than in terms of the biologic or social mechanisms of ultimate interest). In particular, they could be well-positioned to propose realistic models for sources of bias in their study. These bias models can then be used to examine the sensitivity of the results to plausible bias combinations, and (more ambitiously) can be used to quantify uncertainty about the observed associations in a manner less misleading than ordinary statistical methods.2–8
We suggest that removing pressure for study reports to delve into general theory could be constructive in allowing the writers more time and the report more space to describe the methods, analyses, and data. The saved space can help ensure that “null” associations are reported in as much detail (with their interval estimates) as other associations, and so reduce publication bias in the general literature. The added detail can facilitate formulation of explanations by readers. A descriptive orientation could also encourage deferral of general explanations to more thorough and comprehensive reviews. When forced to cram a minireview into a study report, study authors can end up biased toward overweighting the evidence that they know well (eg, their own studies) and must inevitably sacrifice thoroughness.
Our suggestion is analogous to suggestions that authors should be under no pressure to offer public health implications of their results. With regard to the latter, it has been argued that policy implications should be reserved for separate articles that synthesize evidence in a balanced fashion, along with costs and benefits of proposed actions.90 Whether considering scientific explanations or policy formulation, rarely if ever does a single study have any implications in isolation from the broader interdisciplinary whole. Yet, for most topics, even an outline of that whole would be too lengthy to include in every research report, and such inclusion would be wastefully redundant.
Our suggestion would specialize the use of contextual theory (biology, sociology, and so on) in epidemiologic papers into 3 major categories. In study reports, contextual theory could be used to justify the study and the choice of analytic models (including bias models), but the theory need not be reviewed in detail; the primary mission would be accurate presentation of methods and data. Reviews would then bear the task of evaluating contextual theories against all lines of evidence and against each other. Finally, policy analyses would integrate review information about likely effect sizes, along with cost considerations, to compare the likely costs and benefits of various actions (including inaction). Some epidemiologists could find such article specialization in conflict with their conception of public health research. Most researchers accept, however, that personal specialization has become necessary, because one “renaissance scientist” can no longer master all the fields that contribute to understanding and control of complex diseases like cancer (pathology, toxicology, genetics, animal experimentation, epidemiology, sociology, statistics, risk assessment, policy analysis, and so on). Article specialization is an analogous necessity when description, analysis, and interpretation of every relevant study cannot be handled by one “renaissance paper.”
1. Gago-Dominguez M, Castelao JE, Yuan J-M, et al. Lipid peroxidation: a novel and unifying concept of the etiology of renal cell carcinoma (United States). Cancer Causes Control
2. Eddy DM, Hasselblad V, Schachter R. Meta-analyisis by the Confidence Profile Method
. New York: Academic Press; 1992.
3. Greenland S. Basic methods for sensitivity analysis of biases. Int J Epidemiol
4. Lash TL, Silliman RA. A sensitivity analysis to separate bias due to confounding from bias due to predicting misclassification by a variable that does both. Epidemiology
5. Greenland S. Sensitivity analysis, Monte Carlo risk analysis, and Bayesian uncertainty assessment. Risk Analysis
6. Lash TL, Fink AK. Semi-automated sensitivity analysis to assess systematic errors in observational epidemiologic data. Epidemiology
7. Phillips CV. Quantifying and reporting uncertainty from systematic errors. Epidemiology
8. Greenland S. The impact of prior distributions for uncontrolled confounding and response bias. J Am Stat Assoc
9. Skrabanek P. The poverty of epidemiology. Perspect Biol Med
10. Skrabanek P. The epidemiology of errors. Lancet
11. Skrabanek P. Has risk-factor epidemiology outlived its usefulness? Am J Epidemiol
12. Skrabanek P. The emptiness of the black box. Epidemiology
13. Savitz DA. In defense of black box epidemiology. Epidemiology
14. Popper KR. The Logic of Scientific Discovery
, 2nd ed. New York: Harper; 1968.
15. Poole C. Low P
-values or narrow confidence intervals: which are more durable? Epidemiology
16. Greenland S. When should epidemiologic regressions use random coefficients? Biometrics
17. Yu MC, Yuan J-M, Ross RK. Epidemiology of renal cell carcinoma. In: Petrovich Z, Baert L, Brady LW, eds. Carcinoma of the Kidney and Testis, and Rare Urologic Malignancies. Innovations in Management
. Berlin: Springer-Verlag; 1999:3–13.
18. Chow WH, Devesa SS, Warren JL, et al. Rising incidence of renal cell cancer in the United States. JAMA
19. Yuan J-M, Castelao JE, Gago-Dominguez M, et al. Hypertension, obesity and their medications in relation to renal cell carcinoma. Br J Cancer
20. Yuan J-M, Castelao JE, Gago-Dominguez M, et al. Tobacco use in relation to renal cell carcinoma. Cancer Epidemiol Biomarker Prev
21. Van Poppel H, Nilsson S, Algaba F, et al. Precancerous lesions in the kidney. Scand J Urol Nephrol Suppl
22. Gago-Dominguez M, Castelao JE, Yuan JM, et al. Increased risk of renal cell carcinoma subsequent to hysterectomy. Cancer Epidemiol Biomarker Prev
23. Lindblad P, Mellemgaard A, Schlehofer B, et al. International renal-cell cancer study. V. Reproductive factors, gynecologic operations and exogenous hormones. Int J Cancer
24. Chow W-H, McLaughlin JK, Mandel JS, et al. Reproductive factors and the risk of renal cell cancer among women. Int J Cancer
25. Kreiger N, Marrett LD, Dodds L, et al. Risk factors for renal cell carcinoma: results of a population-based case-control study. Cancer Cause Control
26. Lambe M, Lindblad P, Wuu J, et al. Pregnancy and risk of renal cell cancer: a population-based study in Sweden. Br J Cancer
27. Yuan J-M, Gago-Dominguez M, Castelao JE, et al. Cruciferous vegetables in relation to renal cell carcinoma. Int J Cancer
28. Wolk A, Gridley G, Niwa S, et al. International renal cell cancer study. VII. Role of diet. Int J Cancer
29. Lindblad P, Chow WH, Chan J, et al. The role of diabetes mellitus in the aetiology of renal cell cancer. Diabetologia
30. Schlehofer B, Pommer W, Mellemgaard A, et al. International renal-cell-cancer study. VI. The role of medical and family history. Int J Cancer
31. Wideroff L, Gridley G, Mellemkjaer L, et al. Cancer incidence in a population-based cohort of patients hospitalized with diabetes mellitus in Denmark. J Natl Cancer Inst
32. Tydings A, Weiss RR, Lin JH, et al. Renal-cell carcinoma and mesangiocapillary glomerulonephritis presenting as severe pre-eclampsia. N Y State J Med
33. Morrison JJ, Robinson RE. Renal cell carcinoma: an unusual cause of second trimester hypertension. Br J Obstet Gynaecol
34. Gago-Dominguez M, Yuan JM, Castelao JE, et al. Regular use of analgesics is a risk factor for renal cell carcinoma. Br J Cancer
35. Li JL, Okada S, Hamazaki S, et al. Subacute nephrotoxicity and induction of renal cell carcinoma in mice treated with ferric nitrilotriacetate. Cancer Res
36. Deguchi J, Miyamoto M, Okada S. Sex hormone-dependent renal cell carcinogenesis induced by ferric nitrilotriacetate in Wistar rats. Jpn J Cancer Res
37. Okamoto K, Toyokuni S, Uchida K, et al. Formation of 8-hydroxy-2′-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in human renal-cell carcinoma. Int J Cancer
38. Biri H, Cimen MY, Kacmaz M, et al. Antioxidant potential of cancerous human kidney tissues. Cancer Biochem Biophys
39. Balkan J, Kanbagli O, Mehmetcik G, et al. Increased lipid peroxidation in serum and low-density lipoproteins associated with aging in humans. Int J Vitam Nutr Res
40. Reckelhoff JF, Kanji V, Racusen LC, et al. Vitamin E ameliorates enhanced renal lipid peroxidation and accumulation of F2-isoprostanes in aging kidneys. Am J Physiol
41. Yagi K. Female hormones as natural antioxidants—a survey of our research. Acta Biochim Pol
42. Ide T, Tsutsui H, Ohashi N, et al. Greater oxidative stress in healthy young men compared with premenopausal women. Arterioscler Thromb Vasc Biol
43. Knight JA, Smith SE, Kinder VE, et al. Reference intervals for plasma lipoperoxides: age-, sex-, and specimen-related variations. Clin Chem
44. Dincer Y, Ozen E, Kadioglu P, et al. Effect of sex hormones on lipid peroxidation in women with polycystic ovary syndrome, healthy women, and men. Endocr Res
45. Toyokuni S, Okada S, Hamazaki S, et al. Combined histochemical and biochemical analysis of sex hormone dependence of ferric nitrilotriacetate-induced renal lipid peroxidation in ddY mice. Cancer Res
46. Mehrotra S, Ling KL, Bekele Y, et al. Lipid hydroperoxide and markers of renal disease susceptibility in African-Caribbean and Caucasian patients with Type 2 diabetes mellitus. Diabetic Med
47. Health, United States.
Hyattsville, MD: National Center for Health Statistics; 1998.
48. Dobrian AD, Davies MJ, Prewitt RL, et al. Development of hypertension in a rat model of diet-induced obesity. Hypertension
49. Van Gaal LF, Vertommen J, De Leeuw IH. The in vitro oxidizability of lipoprotein particles in obese and non-obese subjects. Atherosclerosis
50. Kuno T, Hozumi M, Morinobu T, et al. Antioxidant vitamin levels in plasma and low density lipoprotein of obese girls. Free Radic Res
51. Vasankari T, Fogelholm M, Oja P, et al. Effect of weight reduction on LDL oxidation. Med Sci Sports Exerc
52. Dietrich M, Block G, Hudes M, et al. Antioxidant supplementation decreases lipid peroxidation biomarker F(2)-isoprostanes in plasma of smokers. Cancer Epidemiol Biomarker Prev
53. Olusi SO. Obesity is an independent risk factor for plasma lipid peroxidation and depletion of erythrocyte cytoprotective enzymes in humans. Int J Obesity
54. Vasankari TJ, Kujala UM, Vasankari TM, et al. Reduced oxidized LDL levels after a 10-month exercise program. Med Sci Sports Exerc
55. Usyal M, Bulur H, Sener D, et al. Lipid peroxidation in patients with essential hypertension. Int J Clin Pharmacol Ther Toxicol
56. Kumar KV, Das UN. Are free radicals involved in the pathobiology of human essential hypertension? Free Radic Res Commun
57. Keidar S, Kaplan M, Shapira C, et al. Low density lipoprotein isolated from patients with essential hypertension exhibits increased propensity for oxidation and enhanced uptake by macrophages: a possible role for angiotensin II. Atherosclerosis
58. Zhang D, Okada S, Yu Y, et al. Vitamin E inhibits apoptosis, DNA modification, and cancer incidence induced by iron-mediated peroxidation in Wistar rat kidney. Cancer Res
59. Hashimoto S. Study on the effects of the hypocholesteremic agents, cholestyramine and compactin on the induction of renal tumors in rats by N-ethyl-N-hydroxyethyl nitrosamine. Jpn J Urol
60. Qin X, Zhang S, Zarkovic M, et al. Inhibitory effect of probucol on nephrotoxicity induced by ferric nitrilotriacetate (Fe-NTA) in rats. Carcinogenesis
61. Brude IR, Drevon CA, Hjermann I, et al. Peroxidation of LDL from combined-hyperlipidemic male smokers supplied with omega-3 fatty acids and antioxidants. Arterioscler Thromb Vasc Biol
62. Salonen R, Nyssonen K, Porkkala-Sarataho E, et al. The Kuopio Atherosclerosis Prevention Study (KAPS): effect of pravastatin treatment on lipids, oxidation resistance of lipoproteins, and atherosclerotic progression. Am J Cardiol
63. De Caterina R, Cipollone F, Filardo FP, et al. Low-density lipoprotein level reduction by the 3-hydroxy-3-methylglutaryl coenzyme-A inhibitor simvastatin is accompanied by a related reduction of F2-isoprostane formation in hypercholesterolemic subjects: no further effect of vitamin E. Circulation
64. Girona J, La Ville AE, Sola R, et al. Simvastatin decreases aldehyde production derived from lipoprotein oxidation. Am J Cardiol
65. Yoshioka T, Motoyama H, Yamasaki F, et al. Lipid peroxidation and vitamin E levels during pregnancy in rats. Biol Neonate
66. Morris JM, Gopaul NK, Endresen MJ, et al. Circulating markers of oxidative stress are raised in normal pregnancy and pre-eclampsia. Br J Obstet Gynaecol
67. Miller ER III, Appel LJ, Jiang L, et al. Association between cigarette smoking and lipid peroxidation in a controlled feeding study. Circulation
68. al Senaidy AM, al Zahrany YA, al Faqeeh MB. Effects of smoking on serum levels of lipid peroxides and essential fat-soluble antioxidants. Nutr Health
69. Jain SK, McVie R, Jaramillo JJ, et al. The effect of modest vitamin E supplementation on lipid peroxidation products and other cardiovascular risk factors in diabetic patients. Lipids
70. Hubel CA, Roberts JM, Taylor RN, et al. Lipid peroxidation in pregnancy: new perspectives on preeclampsia. Am J Obstet Gynecol
71. Wang YP, Walsh SW, Guo J, et al. The imbalance between thromboxane and prostacyclin in preeclampsia is associated with an imbalance between lipid peroxides and vitamin E in maternal blood. Am J Obstet Gynecol
72. Hickey EJ, Raje RR, Reid VE, et al. Diclofenac induced in vivo nephrotoxicity may involve oxidative stress-mediated massive genomic DNA fragmentation and apoptotic cell death. Free Radic Biol Med
73. Durak I, Karaayvaz M, Cimen MY, et al. Aspirin impairs antioxidant system and causes peroxidation in human erythrocytes and guinea pig myocardial tissue. Human Exp Toxicol
74. Ji B, Masubuchi Y, Horie T. A possible mechanism of naproxen-induced lipid peroxidation in rat liver microsomes. Pharmacol Toxicol
75. Gnarra JR, Zhou S, Merrill MJ, et al. Post-transcriptional regulation of vascular endothelial growth factor mRNA by the product of the VHL tumor suppressor gene. Proc Natl Acad Sci U S A
76. Iliopoulos O, Levy AP, Jiang C, et al. Negative regulation of hypoxia-inducible genes by the von Hippel-Lindau protein. Proc Natl Acad Sci U S A
77. Siemeister G, Weindel K, Mohrs K, et al. Reversion of deregulated expression of vascular endothelial growth factor in human renal carcinoma cells by von Hippel-Lindau tumor suppressor protein. Cancer Res
78. Sarada SK, Dipti P, Anju B, et al. Antioxidant effect of beta-carotene on hypoxia induced oxidative stress in male albino rats. J Ethnopharmacol
79. Ruef J, Hu ZY, Yin LY, et al. Induction of vascular endothelial growth factor in balloon-injured baboon arteries. A novel role for reactive oxygen species in atherosclerosis. Circulation Res
80. Yang AH, Oberley TD, Oberley LW, et al. In vitro modulation of antioxidant enzymes in normal and malignant renal epithelium. In Vitro Cell Dev Biol
81. Oberley TD, Toyokuni S, Szweda LI. Localization of hydroxynonenal protein adducts in normal human kidney and selected human kidney cancers. Free Radic Biol Med
82. Block G, Dietrich M, Norkus EP, et al. Factors associated with oxidative stress in human populations. Am J Epidemiol
83. McLaughlin JK, Chow WH, Mandel JS, et al. International renal-cell cancer study. VIII. Role of diuretics, other anti-hypertensive medications and hypertension. Int J Cancer
84. Chow WH, Gridley G, Fraumeni JFJr, et al. Obesity, hypertension, and the risk of kidney cancer in men. N Engl J Med
85. Graaf MR, Beiderbeck AB, Egberts ACG, et al. The risk of cancer in users of statins. Proc Am Soc Clin Oncol 2003;22:846, 39th Annual Meeting, Chicago, May 31–June 3; Abstract 3400 and oral presentation.
86. Elisaf M, Mikhailidis DP. Statins and renal function. Angiology
87. McKenney JM. Potential nontraditional applications of statins. Ann Pharmacother
88. van den Akker JM, Bredie SJ, Diepenveen SH, et al. Atorvastatin and simvastatin in patients on hemodialysis: effects on lipoproteins, C-reactive protein and in vivo oxidized LDL. J Nephrol
89. Stewart JH, Buccianti G, Agodoa L, et al. Cancers of the kidney and urinary tract in patients on dialysis for end-stage renal disease: analysis of data from the United States, Europe, and Australia and New Zealand. J Am Soc Nephrol
90. Rothman KJ. Policy recommendations in epidemiologic research papers. Epidemiology
On Miasmas and Astrology
“For want of knowing any other cause, epidemics were attributed by the ancients to the atmosphere, without any evidence; just as political and social events were believed to be occasioned by the stars.” - JOHN SNOW
This article has been cited 31 time(s).
Journal of Epidemiology and Community HealthEstimating the potential impacts of intervention from observational data: methods for estimating causal attributable risk in a cross-sectional analysis of depressive symptoms in Latin AmericaJournal of Epidemiology and Community Health
Cancer Epidemiology Biomarkers & PreventionRole of lipid peroxidation in the epidemiology and prevention of breast cancerCancer Epidemiology Biomarkers & Prevention
American Journal of EpidemiologyInvited Commentary: Coming Out of the BoxAmerican Journal of Epidemiology
Environmental Health PerspectivesCommunity-initiated breast cancer and environment studies and the precautionary principleEnvironmental Health Perspectives
American Journal of EpidemiologyInvited commentary: Considerations about specificity of associations, causal pathways, and heterogeneity in multilevel thinkingAmerican Journal of Epidemiology
Cancer Epidemiology Biomarkers & Prevention
Reflections on publication criteria for genetic association studies
Cancer Epidemiology Biomarkers & Prevention, 14(6):
International Journal of EpidemiologyThe triumph of the null hypothesis: epidemiology in an age of changeInternational Journal of Epidemiology
British Journal of NutritionDietary fat and risk of renal cell carcinoma in the USA: a case-control studyBritish Journal of Nutrition
Medicine Health Care and PhilosophyEpidemiology and causationMedicine Health Care and Philosophy
Free Radical Biology and MedicineLipid peroxidation and renal cell carcinoma: Further supportive evidence and new mechanistic insightsFree Radical Biology and Medicine
Journal of Epidemiology and Community HealthA life-course approach to measuring socioeconomic position in population health surveillance systemsJournal of Epidemiology and Community Health
Preventive Veterinary MedicineLinking causal concepts, study design, analysis and inference in support of one epidemiology for population healthPreventive Veterinary Medicine
Archives of Neurology
Lost in Translation Epidemiology, Risk, and Alzheimer Disease
Archives of Neurology, 67(1):
British Medical Journal
Taking folate in pregnancy and risk of maternal breast cancer - What's in a name? Reply
British Medical Journal, 330():
Breast Cancer ResearchLipid peroxidation, oxidative stress genes and dietary factors in breast cancer protection: a hypothesisBreast Cancer Research
Pediatric Blood & CancerGenetic polymorphisms and disease preventionPediatric Blood & Cancer
Environmental Health PerspectivesEnvironmental determinants of infectious disease: A framework for tracking causal links and guiding public health researchEnvironmental Health Perspectives
Scandinavian Journal of Work Environment & Health
Shift work, chronodisruption and cancer?-the IARC 2007 challenge for research and prevention and 10 theses from the Cologne Colloquium 2008
Scandinavian Journal of Work Environment & Health, 35(1):
International Journal of Environmental Research and Public HealthDental Caries Risk Studies Revisited: Causal Approaches Needed for Future InquiriesInternational Journal of Environmental Research and Public Health
Veterinary JournalRisk factors for strap-related lesions in working donkeys at the World Heritage Site of Petra in JordanVeterinary Journal
International Journal of EpidemiologyCommentary: Rethinking epidemiologyInternational Journal of Epidemiology
International Journal of EpidemiologyIs aldehyde dehydrogenase 2 a credible genetic instrument for alcohol use in Mendelian randomization analysis in Southern Chinese men?International Journal of Epidemiology
British Journal of NutritionEvaluating the effect of measurement error when using one or two 24 h dietary recalls to assess eating out: a study in the context of the HECTOR projectBritish Journal of Nutrition
EpidemiologyAn Epistemologist Among the EpidemiologistsEpidemiology
EpidemiologyPresents Can Come in Black Boxes, tooEpidemiology
Sexually Transmitted DiseasesThe Atlanta Urban Adolescent Network Study: A Network View of STD PrevalenceSexually Transmitted Diseases
EpidemiologyEco-Epidemiology: Thinking Outside the Black BoxEpidemiology
EpidemiologyWhen Can Risk-Factor Epidemiology Provide Reliable Tests?Epidemiology
© 2004 Lippincott Williams & Wilkins, Inc.