Enter your Email address:
Wolters Kluwer Health may email you for journal alerts and information, but is committed
to maintaining your privacy and will not share your personal information without
You currently have
no recent searches
You currently have no recent searches
Damm, M.; Stehr, S. N.; Huebler, A.; Panousis, P.; Koch, T.; Deussen, A.
University Hospital Carl Gustav Carus, Department of Anaesthesiology and Intensive Care, Dresden, Germany
Background and Goal of Study: Ecto‐5'‐nucleotidase (CD73) and A2B‐adenosine receptors (A2B‐AR) are involved in both ischemic preconditioning and anaesthetic‐induced preconditioning [1; 2]. This study focused on sevoflurane (sevo) ‐induced preconditioning in CD73 (CD73‐KO) and A2B‐AR (A2BAR‐KO) knockout mice.
Materials and Methods: Experiments were performed with isolated hearts from 12 (6‐wk‐old) male C57BL/6 (wild type = WT), 12 (6‐wk‐old) male CD73‐KO and 12 (6‐wk‐old) male A2BAR‐KO mice. In all three groups six control hearts were matched to six hearts subjected to 15 min of 2.8 vol. % sevo. Infarction was induced by 60 min of left coronary artery occlusion followed by 30 min reperfusion. Ventricular pressure, +dP/dtmax, heart rate, coronary flow and coronary perfusion pressure were continuously measured. Arterial and venous perfusate samples were collected. The area at risk (AAR) and the infarct size were determined by microspheres and propidium iodide staining. Data analysis was performed using Two‐Way‐ANOVA and post‐hoc analysis by Bonferroni.
Results and Discussion: Infarct size in the WT‐control group was 67.3 ± 2.3 % of AAR. Control hearts from CD73‐KO (55.3 ± 2.3 % of AAR) and A2BAR‐KO (66.2 ± 1.8 % of AAR) showed similar infarct sizes. Application of sevo in WT reduced infarction significantly (33.6 ± 2.1 % vs. 67.3 ± 2.3 % of AAR; with sevo vs. without sevo; n=6 each; p ≤ 0.01). Sevo‐induced preconditioning failed in CD73‐KO (52.3 ± 3.6 % vs. 55.3 ± 2.3 % of AAR; CD73‐KO with sevo vs. CD73‐KO without sevo; n=6 each) and in A2BAR‐KO (70.3 ± 1.7 % vs. 66.2 ± 1.8 % of AAR; A2BAR‐KO with sevo vs. A2BAR‐KO without sevo; n=6 each).
Conclusion(s): These data underlines the major role of CD73 and A2B‐AR in sevo‐induced preconditioning in the isolated mouse heart.
Acknowledgements: CD73‐KO and A2BAR‐KO were kindly provided by Prof. Triantafyllos Chavakis and Dr. David Köhler. This study was supported by the ESA research grant.
© 2011 European Society of Anaesthesiology
Colleague's E-mail is Invalid
Your Name: (optional)
Separate multiple e-mails with a (;).
Thought you might appreciate this item(s) I saw at European Journal of Anaesthesiology (EJA).
Send a copy to your email
Your message has been successfully sent to your colleague.
Some error has occurred while processing your request. Please try after some time.
An Existing Folder
A New Folder
The item(s) has been successfully added to "".
Login with your LWW Journals username and password.
Username or Email:
Enter and submit the email address you registered with. An email with instructions to reset your password will be sent to that address.
Link to reset your password has been sent to specified email address.
What does "Remember me" mean?
By checking this box, you'll stay logged in for
days or until you logout. You'll get easier access to your articles, collections,
media, and all your other content, even if you close your browser or shut down your
To protect your most sensitive data and activities (like changing your password),
we'll ask you to re-enter your password when you access these services.
What if I'm on a computer that I share with others?
If you're using a public computer or you share this computer with others, we recommend
that you uncheck the "Remember me" box.
Save my selection