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Effects of Propofol on Expression of Hippocampal Neuronal Nitric Oxide Synthase and Carboxy-Terminal PDZ Ligand of Neuronal Nitric Oxide Synthase in Stressed Rats Undergoing Electroconvulsive Shock

Lv, Feng MSm*; Shen, Yi-wei MSm*; Peng, Li-hua MD*; Li, Ping MD*; Luo, Jie MD*; Wei, Ke MD*; Li, Wei MD; Chen, Jing MSm*; Min, Su BSm*

doi: 10.1097/YCT.0b013e318290fa17
Original Studies

Objectives This study aimed at investigating the effects of propofol on the expression of hippocampal neuronal nitric oxide synthase (nNOS) and carboxy-terminal PDZ ligand of nNOS (CAPON) in stressed rats after electroconvulsive shock (ECS).

Methods Sprague-Dawley rats were stressed repeatedly for 28 days to establish a stressed model. Forty stressed rats were randomly divided into 4 groups (n = 10 for each group): the stressed group (unapplied group), propofol group (applied with intraperitoneal injection of propofol 80 mg/kg for 7 days), ECS group (applied with ECS and intraperitoneal injection of normal saline 8 mL/kg), or propofol + ECS group (applied with ECS and intraperitoneal injection of propofol 80 mg/kg). Sucrose preference test, open-field test, and Morris water maze were used to assess behavioral changes. Immunohistochemistry was carried out to measure the expression of nNOS and CAPON in hippocampal CA1, CA3, and DG areas.

Results Rats in the ECS and propofol + ECS groups had higher sucrose preference percentages and open-field scores when compared with the stressed group. Rats in the ECS group exhibited longer escape latency, shorter space exploration time, up-regulated expression of nNOS, down-regulated expression of CAPON, and increased ratio of nNOS/CAPON in hippocampus. Compared with the ECS group, the propofol + ECS group exhibited shorter escape latency, longer space exploration time, down-regulated expression of nNOS, up-regulated expression of CAPON in hippocampus, and lower nNOS/CAPON values.

Conclusions Propofol may alleviate ECS-induced impairment of learning and memory in stressed rats by inhibiting excessive expression of nNOS and enhancing the expression of CAPON to maintain the balance between nNOS and CAPON in hippocampus.

From the *Department of Anesthesiology, the First Affiliated Hospital of Chongqing Medical University, and †Department of Anesthesiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Received for publication December 22, 2012; accepted February 21, 2013.

Reprints: Su Min, BSm, Department of Anesthesiology, the First Affiliated Hospital of Chongqing Medical University, 1st Youyi Rd, Yuan Jia Gang, Chongqing 400016, China (e-mail: minsu89011068@yahoo.com.cn).

This work was supported by the National Natural Science Foundation of China (no. 30972831 and 81271501).

The authors have no conflicts of interest to report.

© 2013 by Lippincott Williams & Wilkins