Skip Navigation LinksHome > June 2012 - Volume 28 - Issue 2 > Effects of Electroconvulsive Stimulation on Long-Term Potent...
Journal of ECT:
doi: 10.1097/YCT.0b013e31824a47ca
Original Studies

Effects of Electroconvulsive Stimulation on Long-Term Potentiation and Synaptophysin in the Hippocampus of Rats With Depressive Behavior

Li, Wei MD*; Liu, Li MD*; Liu, Yuan-yuan MSm*; Luo, Jie MD*; Lin, Jing-yan MD; Li, Xiao MSm*; Wang, Bin MD*; Min, Su BSm*

Collapse Box

Abstract

Objectives: To evaluate the effects of electroconvulsive stimulation (ECS) on long-term potentiation (LTP) and synaptophysin (SYP) in the hippocampus of rats under chronic unpredictable mild stress (CUMS).

Methods: Sixty Sprague-Dawley rats were randomly divided into three groups (n = 20 for each group): the control group (no intervention), the CUMS group (subjected to 28 days of CUMS exposure followed by 7 days of mock ECS treatment), and the CUMS + ECS group (subjected to 28 days of CUMS exposure followed by 7 days of ECS treatment). Depressive behavior was assessed by a sucrose preference test and an open-field test. The LTP levels in rat hippocampal slices were examined through electrophysiological experiments. The SYP mean density in the hippocampal CA3 region was detected by immunohistochemistry. Hippocampal SYP mRNA was assessed through reverse transcriptase–polymerase chain reaction.

Results: Chronic unpredictable mild stress diminished sucrose preference and reduced measures of locomotor activity. In addition, CUMS impaired LTP in the hippocampal CA1 region and significantly decreased SYP mean density in the hippocampal CA3 region and hippocampal SYP mRNA levels. Electroconvulsive stimulation improved these harmful behavioral effects and ameliorated LTP impairment, as well as stabilized SYP mean density in the hippocampal CA3 region and hippocampal SYP mRNA levels.

Conclusions: These results suggest that ECS can reverse the harmful behavioral effects of CUMS in rats and that the behavioral alterations induced by ECS and/or CUMS may be associated with hippocampal neuroplasticity and SYP levels.

© 2012 by Lippincott Williams & Wilkins

Login

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.