Schairer, Kim S.1; Feeney, M. Patrick2,3; Sanford, Chris A.4
1Audiology and Speech-Language Pathology Service, James H. Quillen Veteran Affairs Medical Center, Mountain Home, Tennessee, USA; 2National Center for Rehabilitative Auditory Research, Portland Veterans Affairs Medical Center, Portland, Oregon, USA; 3Department of Otolaryngology, Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon, USA; and 4Department of Communication Sciences and Disorders, Idaho State University, Pocatello, Idaho, USA.
ACKNOWLEDGMENTS: This work was supported by Department of Veterans Affairs Rehabilitation Research and Development Service Research Enhancement Award Program.
The contents do not represent the views of the Department of Veterans Affairs or of the U.S. Government.
Address for correspondence: Kim S. Schairer, Audiology, James H. Quillen Veteran Affairs Medical Center, Box 4000, Mountain Home, TN 37684, USA. E-mail: firstname.lastname@example.org
Received February 12, 2013
Accepted May 16, 2013
MEMR MEASUREMENTS OBTAINED WITH A PURE-TONE PROBE
A standard clinical immittance test battery includes measurements of the acoustic reflex, or more specifically, the middle ear muscle reflex (MEMR). The MEMR is the contraction of the stapedius muscle in response to high-level acoustic stimulation. Clinical MEMR measurements are made according to the American National Standards Institute standard for immittance instruments (S3.39–2012) by use of a 226-Hz probe tone in conjunction with a reflex-activating stimulus presented to the ipsilateral or contralateral ear. The MEMR is a bilateral response, which means that presenting the activator to one ear will elicit the response in both ears. In traditional clinical measurements, the reflex activator stimulus is a pure tone (500, 1000, 2000, or 4000 Hz) or a broadband noise, and the MEMR is measured at tympanometric peak pressure (TPP) as measured on the tympanogram. If the admittance of the test ear decreases by a criterion amount in the presence of the activator, for example, 0.02 to 0.03 mmho, the reflex is considered to be present. The lowest level at which an activator is presented and reliably elicits at least the criterion change in admittance is considered the MEMR threshold. The MEMR is typically present in ears with pure-tone behavioral thresholds of ≤60 dB HL at the activator frequencies (Gelfand 2009).
Limitations of the Pure-Tone Probe MEMR Test
MEMR threshold measurements obtained using pure-tone probes have an existing evidence base and have been an integral part of the audiological diagnostic test battery for decades. However, there are a few technical limitations of traditional MEMR measurements.
First, a 226-Hz probe tone is typically used for MEMR measurements in older children and adults. MEMR threshold varies with probe frequency (McMillan et al., 1985), and due to developmental issues (see Kei et al., this issue, pp. 17S–26S), higher probe-tone frequencies (660, 800, and 1000 Hz) can be used to improve MEMR detection in infants (Weatherby & Bennett 1980; Kankkunen & Liden 1984; Sprague et al. 1985; Hirsch et al. 1992). However, normative data are limited (e.g., Mazlan et al. 2007, 2009), the option is not available on all equipment, and when the option is available, it takes extra time to obtain MEMR measurements with multiple probe-tone frequencies.
Second, although an objective criterion change in admittance is used to determine presence of an MEMR (i.e., 0.02 mmho), the final judgment of whether an MEMR is present relies on a visual, subjective determination by the examiner. The examiner must confirm that the change in admittance is in the correct direction (a decrease, not an increase), time-locked to the stimulus rather than noise from patient movement, and valid if the baseline is unstable. This is not usually a problem for experienced audiologists, but it limits the utility of the test in applications such as newborn hearing screenings that may be performed by nonaudiological personnel. MEMR screening tests and automatic MEMR threshold tests are available in some commercially available equipment. However, they may be limited in activator frequency, and they may be more susceptible to artifact from patient movement because only one presentation of the activator is presented at a given level.
Finally, MEMR decay measurements conducted to test eighth nerve integrity as a screening for retrocochlear pathology are typically conducted with the reflex-activating tone presented at 10 dB above MEMR threshold. Thus, reflex decay cannot be conducted when reflex thresholds are so high that the reflex decay stimulus would exceed the stimulus limits of the system, or if the activator would be presented at such a high level that it could potentially cause hearing loss. There is evidence of temporary and permanent behavioral threshold shifts (TTS and PTS, respectively) in cases in which the activator was presented at a level above 105 dB HL. Hunter et al. (1999) reported TTS at frequencies above 1000 Hz and a PTS at 1000 Hz after an acoustic reflex decay test using a1000 Hz tone at level of 120 dB HL. Arriaga and Luxford (1993) reported a PTS at 2000 Hz after presentation of a 2000 Hz activator at 120 dB HL in one ear of an older patient with hearing loss. Miller et al. (1984) reported PTS and a temporary decrease in speech discrimination in an older patient. Thus, an MEMR test that would result in lower MEMR thresholds would be useful in avoiding high presentation levels for the MEMR decay test.
MEMR MEASUREMENTS OBTAINED WITH A WIDEBAND PROBE
Immittance measurements that are obtained with a wideband probe such as a click or chirp are referred to in this volume as wideband acoustic immittance (WAI). Measuring the MEMR with a wideband probe rather than a single-probe frequency allows the MEMR to be detected simultaneously across several octaves, thus providing a sensitive test of the reflex for adults and infants. A summary of WAI MEMR studies is shown in Table 1. In most studies, the clinical MEMR measurements were made with the GSI-33 or Tympstar devices (Grason-Stadler Inc., Eden Prairie, MN) and a 226 Hz probe. The exceptions were studies by Keefe et al. (2010) and Feeney and Sanford (2005) in which a 1000 Hz probe was used when testing infants. The MEMR criterion was 0.03 mmho in the clinical measurements with the exception of Schairer et al. (2007) in which the criterion was 0.02 mmho. The ER-10C probe (Etymotic Research Inc., Elk Grove Village, IL) was used in the WAI measurements in all studies except the study by Keefe et al. in which a wideband tympanometer (Interacoustics, Assens, Denmark) was used. Most WAI MEMR measurements were made at ambient pressure, with the exception of Keefe et al., in which the MEMR measurements in adults were obtained at ambient pressure and at TPP. Adults in all studies and the children in the Schairer et al. study had normal hearing. Infants in the Feeney and Sanford study passed an otoacoustic emissions (OAEs) screen.
Feeney and Keefe (1999) elicited contralateral (probe right, activator left) MEMR-induced changes in admittance, power reflectance, and absorbed power of a 40-msec chirp probe using a system developed by Keefe et al. (1992). An ER-10C was used to present the probe and record responses in the ear canal. An audiometer was used to present the 1000 and 2000 Hz activators. During data collection, the probe was turned on and off manually on a computer by one experimenter, and the activators were presented manually by a second experimenter. Identification of the MEMR was made by visual inspection of the comparison between an average of WAI responses to eight chirps before the onset of the activator and the WAI responses when the activator was presented. In the presence of the activator, power reflectance increased at low frequencies and often decreased above some transition frequency around 1000 Hz. Admittance and absorbed power decreased at low frequencies and increased above a transition frequency. MEMRs identified in WAI measurements were present at least 8 dB lower than the MEMR thresholds obtained with the pure-tone probe, with the exception of one subject in the 2000 Hz activator condition. In this study, a fixed set of activator levels was used (±8 dB in 2-dB steps relative to clinical MEMR threshold), and WAI MEMR thresholds may have been below the lowest activator level that was presented.
Feeney and Keefe (2001) used the same procedures as they did in 1999, with the exceptions of a larger range of activator levels in the WAI test, white noise rather than pure-tone activators, and two objective statistical methods to identify the presence of the WAI MEMR. The range of activator levels in the WAI MEMR measurements was −32 to +8 dB in 2-dB steps relative to the clinical MEMR threshold. Magnitude and correlation tests were use to detect an MEMR-induced shift in the wideband probe. The MEMR thresholds obtained with the wideband probe were up to 24 dB lower than thresholds obtained with a pure-tone probe depending on the method used to identify the WAI MEMR. In a third study with a larger set of subjects, Feeney et al. (2003) used similar methods to those used by Feeney and Keefe (2001). The range of activator levels in the WAI MEMR measurements in the 2003 study was −24 to +4 dB in 4 dB steps relative to the clinical MEMR threshold. Feeney et al. observed that contralateral MEMR thresholds for 1000 and 2000 Hz activators identified in WAI measurements of admittance and power reflectance were, on average, 13 dB lower than clinical MEMR thresholds. Finally, Feeney et al. (2004) measured WAI MEMR thresholds using a filtered-click probe, a 4000 Hz activator presented through an ER-10C rather than an audiometer, an adaptive MEMR threshold search procedure, and a 16-click baseline. The activator levels were presented in 2 dB steps starting from clinical MEMR threshold or 92 dB HL. The authors observed that average contralateral WAI MEMR thresholds were 3 dB lower than the clinical MEMR thresholds. Ipsilateral WAI MEMR thresholds were obtained but not compared with pure-tone MEMR thresholds.
Schairer et al. (2007) used an automated system to test adults and children using a click probe and ipsilateral activators of 1000 and 2000 Hz and a broadband noise. Activator levels were presented to adults in a fixed range from 16 dB below the maximum output in 4 dB steps up to the maximum output (5 levels total). Step sizes in children were 5 dB and the range included two additional lower levels. In their method, a click probe was present throughout the measurement window, with a baseline of responses to only the clicks, followed by responses to the clicks plus the activator, and finally responses to clicks after the activator was turned off. (A modified version of the stimulus presentation sequence used in Keefe et al. (2010) is shown in Fig. 1.) The probe/activator series was presented three times and responses were averaged across repetitions. The click that was presented directly after activator offset was excluded from analysis to avoid contamination by OAEs that were elicited by the higher activator levels. The difference in wideband admittance and power reflectance between postactivator versus baseline click responses was calculated as a function of frequency in third-octave bands from 320 to 2000 Hz. A repeated-measures analysis of variance was completed to determine whether the shifts in any of the third-octave bands was significantly different from zero. The lowest activator level that produced a significant MEMR shift in any third-octave band was defined as threshold. This analysis was completed at each activator level and frequency, and in each ear. An additional rule was that the highest activator level did not produce a significant shift in a coupler. Schairer et al. reported that WAI MEMR thresholds in adults were lower than 226 Hz probe MEMR thresholds by 2.2 to 4.0 dB. No differences between WAI and clinical MEMR thresholds were observed in the child group, however, the authors noted that the results should be interpreted with caution due to the small sample size and large standard error for tonal activators.
WAI FOR INFANT MEMR MEASUREMENTS
Wideband MEMR measurements have potential for use in newborn hearing screenings because they can be made without pressurization of the ear canal, and because objective decision rules for MEMR identification can be used in the data collection software. Such objective decision rules could be used in automated systems used by nonaudiological personnel. Another advantage of the broadband response is that changes in WAI in the presence of the activator can be observed regardless of the frequency range of maximum shift, which can be affected by age and middle ear status. For example, Feeney and Sanford (2005) found that the best frequency range over which to detect a wideband contralateral MEMR, elicited with a broadband noise, was 250 to 2000 Hz for adults, and 1000 to 8000 Hz for 6-week-old infants. With better detection of the MEMR and objective, automated protocols for newborn hearing screening, infants could be screened for both cochlear and retrocochlear involvement with one probe insertion using a system that has middle ear, OAE, and MEMR tests. Such a protocol would potentially identify infants in the well-baby nursery who may have auditory neuropathy and otherwise would be missed by an OAE-only screen. Individuals with auditory neuropathy often have present OAEs and absent MEMR and auditory brainstem responses (Hood 1999; Berlin et al. 2005).
Keefe et al. (2010) used a wideband tympanometer and an improved detection algorithm to identify the MEMR in a larger sample of newborn infants. In their method, the activator was pulsed and alternated with the click probe as shown in Figure 1, and a maximum likelihood technique in two frequency ranges (low and high) was used to detect the MEMR. They found that a combination of WAI tests of middle ear function and wideband MEMR tests could be used to predict newborn hearing screening outcomes. For further discussion of wideband MEMR measurements in infants, please see Hunter et al. (this issue, pp. 36S–42S).
NEED FOR FURTHER RESEARCH AND FUTURE DIRECTIONS
Wideband MEMR measurements have the same potential for clinical use as their pure-tone probe counterparts. Early studies suggest that WAI measures of MEMR may result in lower reflex thresholds. However, more normative data are needed for ipsilateral and contralateral WAI MEMR thresholds in adults and children with varying degrees of hearing loss. In addition, because WAI changes as a function of age in infancy (see Kei et al., this issue, pp. 17S–26S) and may change in the elderly (Feeney & Sanford 2004), the effect of age on WAI MEMR thresholds must be examined. Most of the available WAI MEMR data were collected with nonpressurized ear canals whereas 226 Hz probe measurements are typically made at TPP. More data are needed on WAI measurements of MEMR thresholds at ambient and TPP. There are currently no data available for WAI MEMR decay tests. Studies that include ears with retrocochlear pathology are required to estimate sensitivity and specificity of WAI MEMR threshold and decay tests for separating ears with cochlear from retrocochlear pathology. Finally, automated systems with objective detection of WAI MEMR thresholds could be developed for use by nonaudiological personnel in newborn hearing screening programs and by audiologists to save time during standard hearing evaluations.
American National Standards Institute Specifications for instruments to measure aural acoustic impedance and admittance (aural acoustic immittance) (ANSI S3.39–1987–R2012). (2012); New York, NY American National Standards Institute
Arriaga M. A., Luxford W. M.. Impedance audiometry and iatrogenic hearing loss. Otolaryngol Head Neck Surg. (1993); 108:70–72
Berlin C. I., Hood L. J., Morlet T., et al. Absent or elevated middle ear muscle reflexes in the presence of normal otoacoustic emissions: A universal finding in 136 cases of auditory neuropathy/dys-synchrony. J Am Acad Audiol. (2005); 16:546–553
Feeney M. P., Keefe D. H.. Acoustic reflex detection using wide-band acoustic reflectance, admittance, and power measurements. J Speech Lang Hear Res. (1999); 42:1029–1041
Feeney M. P., Keefe D. H.. Estimating the acoustic reflex threshold from wideband measures of reflectance, admittance, and power. Ear Hear. (2001); 22:316–332
Feeney M. P., Keefe D. H., Marryott L. P.. Contralateral acoustic reflex thresholds for tonal activators using wideband energy reflectance and admittance. J Speech Lang Hear Res. (2003); 46:128–136
Feeney M. P., Keefe D. H., Sanford C. A.. Wideband reflectance measures of the ipsilateral acoustic stapedius reflex threshold. Ear Hear. (2004); 25:421–430
Feeney M. P., Sanford C. A. Age effects in the human middle ear: Wideband acoustical measures,. J Acoust Soc Am. (2004); 116:3546–3558
Feeney M. P., Sanford C. A.. Detection of the acoustic stapedius reflex in infants using wideband energy reflectance and admittance. J Am Acad Audiol. (2005); 16:278–290
Gelfand S. A.. J. Katz. The acoustic reflex. In: Handbook of Clinical Audiology. (2009); 6th ed. Baltimore, MD Williams & Wilkins pp. 189–221
Hirsch J. E., Margolis R. H., Rykken J. R.. A comparison of acoustic reflex and auditory brain stem response screening of high-risk infants. Ear Hear. (1992); 13:181–186
Hood L. J.. A review of objective methods of evaluating auditory neural pathways. Laryngoscope. (1999); 109:1745–1748
Hunter L. L., Ries D. T., Schlauch R. S., et al. Safety and clinical performance of acoustic reflex tests. Ear Hear. (1999); 20:506–514
Kankkunen A., Lidén G.. Ipsilateral acoustic reflex thresholds in neonates and in normal-hearing and hearing-impaired pre-school children. Scand Audiol. (1984); 13:139–144
Keefe D. H., Fitzpatrick D., Liu Y. W., et al. Wideband acoustic-reflex test in a test battery to predict middle-ear dysfunction. Hear Res. (2010); 263:52–65
Keefe D. H., Ling R., Bulen J. C.. Method to measure acoustic impedance and reflection coefficient. J Acoust Soc Am. (1992); 91:470–485
Mazlan R., Kei J., Hickson L., et al. High frequency immittance findings: Newborn versus six-week-old infants. Int J Audiol. (2007); 46:711–717
Mazlan R., Kei J., Hickson L.. Test-retest reliability of the acoustic stapedial reflex test in healthy neonates. Ear Hear. (2009); 30:295–301
McMillan P. M., Bennett M. J., Marchant C. D., et al. Ipsilateral and contralateral acoustic reflexes in neonates. Ear Hear. (1985); 6:320–324
Miller M. H., Hoffman R. A., Smallberg G. J.. Stapedial reflex testing and partially reversible acoustic trauma. Hearing Instrum. (1984); 35:15–49
Schairer K. S., Ellison J. C., Fitzpatrick D., et al. Wideband ipsilateral measurements of middle-ear muscle reflex thresholds in children and adults. J Acoust Soc Am. (2007); 121:3607–3616
Sprague B. H., Wiley T. L., Goldstein R.. Tympanometric and acoustic-reflex studies in neonates. J Speech Hear Res. (1985); 28:265–272
Weatherby L. A., Bennett M. J.. The neonatal acoustic reflex. Scand Audiol. (1980); 9:103–110