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Measurement of Ribavirin Plasma Concentrations by High-performance Liquid Chromatography Using a Novel Solid-phase Extraction Method in Patients Treated for Chronic Hepatitis C

Morello, Judit PharmD*; Rodríguez-Nóvoa, Sonia PhD*; Cantillano, Ana L Rendon PhD*; González-Pardo, Gema*; Jiménez, Inmaculada PhD†; Soriano, Vincent PhD*

doi: 10.1097/FTD.0b013e31815bddf3
Original Article

Ribavirin (RBV) in combination with pegylated interferon is the current standard treatment for chronic hepatitis C. Exposure to RBV seems crucial for achieving the best virologic response. However, RBV may cause anemia in a dose-dependent manner. Therefore, monitoring RBV plasma levels could be useful for individual tailoring of RBV dosing. A rapid assay was developed to quantify RBV using high-performance liquid chromatography and ultraviolet detection. Extraction of RBV from plasma was performed using a novel method based on ultrafiltration in one step that allows direct injection into the high-performance liquid chromatography without any prior steps of dryness or reconstitution. The method was validated over the range of 0.05 to 5.0 μg/mL following the EMEA Validation of Analytical Procedures (CPMP/ICH/281/95) recommendations. The clinical interest of this assay was evaluated in a subset of patients under RBV therapy. Mean RBV plasma concentrations at steady state were higher in responders compared with nonresponders (3-3.2 μg/mL versus 2.2-2.3 μg/mL).

From the *Services of Infectious Diseases and †Pharmacy, Hospital Carlos III, Madrid, Spain.

Received for publication February 23, 2007; accepted September 5, 2007.

This work was supported in part by grants from Fundación Investigación y Educación en SIDA (IES), Red de Investigación en SIDA (RIS), Fondo de Investigación Sanitaria (FIS), and Agencia Laín Entralgo.

Correspondence: Sonia Rodríguez-Nóvoa, PhD, Department of Infectious Diseases, Hospital Carlos III Calle Sinesio Delgado 10 28029 Madrid, Spain (e-mail: sonia_r_novoa@hotmail.com).

© 2007 Lippincott Williams & Wilkins, Inc.