Skip Navigation LinksHome > August 2005 - Volume 27 - Issue 4 > Determination of Nevirapine and Efavirenz in Plasma Using GC...
Therapeutic Drug Monitoring:
doi: 10.1097/01.ftd.0000164613.91555.2e
Original Article

Determination of Nevirapine and Efavirenz in Plasma Using GC/MS in Selected Ion Monitoring Mode

Lemmer, Patrick BSc*; Schneider, Serge PhD*; Schuman, Marc PhD*; Omes, Christine PharmD†; Arendt, Vic MD‡; Tayari, Jean-Claude PharmD†; Fundira, Léon PharmD†; Wennig, Robert PhD*

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An accurate, selective, and sensitive method for the determination of the nonnucleoside reverse transcriptase inhibitors (NNRTIs) nevirapine (nvp) and efavirenz (efv) in human plasma using gas chromatography-mass spectroscopy in selected ion monitoring mode (GC/MS-SIM) was developed. Solid-phase extraction (SPE) gave extraction yields near 100% for both nvp and efv, and calibration curves were linear over the therapeutic concentration ranges. Variation of intraday and interday precision was below 5%. Intraday and interday inaccuracies varied between 0.6% and 10.4%. The lower limits of detection using a 200-μL plasma sample were 27 ng/mL for nvp and 26 ng/mL for efv, and the lower limits of quantification were 54 ng/mL and 72 ng/mL, respectively. The method was applied to the determination of nvp and efv in plasma specimens of 73 patients in HIV stage III or IV and on antiretroviral treatment in Kigali, Rwanda.

© 2005 Lippincott Williams & Wilkins, Inc.


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