Skip Navigation LinksHome > March/April 2014 - Volume 25 - Issue 2 > Flow Cytometric Analysis of Regulatory T Cells During Hypose...
doi: 10.1097/DER.0000000000000022

Flow Cytometric Analysis of Regulatory T Cells During Hyposensitization of Acquired Allergic Contact Dermatitis

Fraser, Kathleen BHSc; Abbas, Mariam MD; Hull, Peter R. PhD, MB, BCh, FFDerm(SA), FRCPC

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Background: We previously demonstrated that repeated intradermal steroid injections administered at weekly intervals into positive patch-test sites induce hyposensitization and desensitization.

Objective: To examine changes in CD4+CD25+CD127lo/ regulatory T cells during the attenuation of the patch-test response.

Methods: Ten patients with known allergic contact dermatitis were patch tested weekly for 10 weeks. The patch-test site was injected intradermally with 2 mg triamcinolone. At weeks 1 and 7, a biopsy was performed on the patch-test site in 6 patients, and flow cytometry was performed assessing CD4+CD25+CD127lo/ regulatory T cells. Secondary outcomes were clinical score, reaction size, erythema, and temperature. Statistical analysis included regression, correlation, and repeated-measures analysis of variance.

Results: The percentage of CD4+CD25+CD127lo/ regulatory T cells, measured by flow cytometry, increased from week 1 to week 7 by an average of 19.2%. The average grade of patch-test reaction decreased from +++ (vesicular reaction) to ++ (palpable erythema). The mean drop in temperature following treatment was 0.28°C per week. The mean area decreased 8.6 mm2/wk over 10 weeks.

Conclusions: Intradermal steroid injections of weekly patch-test reactions resulted in hyposensitization of the allergic contact dermatitis reaction. CD4+CD25+CD127lo/ regulatory T cells showed a tendency to increase; however, further studies are needed to determine if this is significant.

© 2014 American Contact Dermatitis Society

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