Skip Navigation LinksHome > September 2014 - Volume 33 - Issue 9 > Moesin Expression in Fibrosis in the Mouse Cornea After Ster...
doi: 10.1097/ICO.0000000000000179
Basic Investigation

Moesin Expression in Fibrosis in the Mouse Cornea After Sterile Mechanical Trauma or Infection

Zhu, Hong-Y. MD, PhD*; Ng, Jennifer BSc*; Salleh, Shuhaida M. BSc*; Aung, Thet T. MBBS, MSc*; Htoon, Myint H. BDS, PhD*; Beuerman, Roger W. PhD*,†

Collapse Box


Purpose: The aim of this study was to compare the expression patterns of 3 important biochemical characteristics of fibrosis–moesin, transforming growth factor (TGF)-β1, and α-smooth muscle actin (SMA) in the mouse cornea with fibrosis induced by common etiologies—sterile mechanical injury and infection.

Methods: Corneas of 8-week-old C57BL6 mice were either wounded after an anterior keratectomy or were infected by Pseudomonas aeruginosa, and the animals were killed on days 2 and 7, and on weeks 2 and 4 after the procedure. Western blot and immunofluorescence were used to analyze the expression of moesin and phospho-moesin, and the presence of myofibroblasts identified by the expression of α-SMA in the corneal stroma. The expression of TGF-β1 was analyzed by immunofluorescence.

Results: By immunofluorescent analysis, TGF-β1, α-SMA, and phospho-moesin were not detected in the normal corneal stroma. However, after either treatment, TGF-β1 expression increased, along with phospho-moesin in the wounded corneal stroma until day 7, and decreased after week 2. No expression of TGF-β1 and phospho-moesin was found at postoperative week 4. Moesin expression increased until week 2. Myofibroblasts positive for α-SMA were detected on day 2 until week 4 and peaked at week 2. Western blot analysis confirmed the immunofluorescent data for moesin, phospho-moesin, and α-SMA.

Conclusions: The similar expression pattern of moesin, phospho-moesin, TGF-β1, and α-SMA in the mouse cornea with fibrosis caused by sterile mechanical injury or infection indicated a role for moesin signaling in corneal fibrosis. Interference with the action of moesin may be a potential approach for intervention strategies to avert fibrosis after infection or mechanical injury.

© 2014 by Lippincott Williams & Wilkins.


Article Tools


Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.