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Cornea:
doi: 10.1097/ICO.0000000000000156
Clinical Science

Postmortem Ultrastructural Analysis of a Cornea Transplanted With Descemet Membrane Endothelial Keratoplasty

Livny, Eitan MD*,†; Parker, Jack S. MD*,†; van der Kaaij, Mariëlle BASc†,‡; Haasdijk, Elize D. Ing§; van der Wees, Jacqueline PhD†,‡; Bruinsma, Marieke PhD; Melles, Gerrit R.J. MD, PhD*,†,‡

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Abstract

Purpose:

The aim of this study was to describe the ultrastructure of the host–donor interface in the eye of a recently deceased patient, who had undergone Descemet membrane endothelial keratoplasty.

Methods:

The eye was enucleated postmortem, and after standard decontamination, the corneoscleral button was excised, cut into 4 quadrants, and processed for light and transmission electron microscopy evaluation.

Results:

Transmission electron microscopy revealed close attachment of the donor's Descemet membrane to the host's stroma and projection of stromal collagen fibers into the interfacial matrix, resembling a normal “virgin” corneal architecture.

Conclusions:

Ultrastructurally, an attached Descemet membrane endothelial keratoplasty graft closely resembles that of an unoperated, healthy eye with no appreciable adventitious or missing structures.

Copyright © 2014 by Lippincott Williams & Wilkins

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