Purpose: The aim of this study was to describe the ultrastructure of the host–donor interface in the eye of a recently deceased patient, who had undergone Descemet membrane endothelial keratoplasty.
Methods: The eye was enucleated postmortem, and after standard decontamination, the corneoscleral button was excised, cut into 4 quadrants, and processed for light and transmission electron microscopy evaluation.
Results: Transmission electron microscopy revealed close attachment of the donor's Descemet membrane to the host's stroma and projection of stromal collagen fibers into the interfacial matrix, resembling a normal “virgin” corneal architecture.
Conclusions: Ultrastructurally, an attached Descemet membrane endothelial keratoplasty graft closely resembles that of an unoperated, healthy eye with no appreciable adventitious or missing structures.
*Melles Cornea Clinic Rotterdam, Rotterdam, the Netherlands;
†Netherlands Institute for Innovative Ocular Surgery, Rotterdam, the Netherlands;
‡Amnitrans Eye Bank Rotterdam, Rotterdam, the Netherlands; and
§Department of Neuroscience, Erasmus Medical Center Rotterdam, the Netherlands.
Reprints: Gerrit R.J. Melles, MD, PhD, Netherlands Institute for Innovative Ocular Surgery, Laan op Zuid 88, 3071 AA, Rotterdam, the Netherlands (e-mail: firstname.lastname@example.org).
G. R. J. Melles is a consultant for DORC International/Dutch Ophthalmic, USA. The remaining authors have no funding or conflicts of interest to disclose.
Received January 23, 2014
Accepted April 13, 2014