Purpose: The aim of this study was to compare the effect on corneal wound healing of 3 differently manufactured blood derivatives [autologous serum (AS), platelet-rich plasma, and serum derived from plasma rich in growth factors (s-PRGF)].
Methods: Scratch wound-healing assays were performed on rabbit primary corneal epithelial cultures and human corneal epithelial cells. Additionally, mechanical debridement of rabbit corneal epithelium was performed. Wound-healing progression was assessed by measuring the denuded areas remaining over time after treatment with each of the 3 blood derivatives or a control treatment.
Results: In vitro data show statistically significant differences in the healing process with all the derivatives compared with the control, but 2 of them (AS and s-PRGF) induced markedly faster wound healing. In contrast, although the mean time required to complete in vivo reepithelization was similar to that of AS and s-PRGF treatment, only wounds treated with s-PRGF were significantly smaller in size from 2.5 days onward with respect to the control treatment.
Conclusions: All 3 blood derivatives studied are promoters of corneal reepithelization. However, the corneal wound-healing progresses differently with each derivative, being faster in vitro under AS and s-PRGF treatment and producing in vivo the greatest decrease in wound size under s-PRGF treatment. These findings highlight that the manufacturing process of the blood derivatives may modulate the efficacy of the final product.
*Department of Cell Biology and Histology, School of Medicine and Dentistry, BioCruces Health Research Institute, University of the Basque Country, Leioa, Spain;
†Department of R&D, Instituto Clínico-Quirúrgico de Oftalmología, Bilbao, Spain;
‡BioCruces Health Research Institute, Cruces University Hospital, Barakaldo, Spain; and
§Department of Ophthalmology, School of Medicine and Dentistry, BioCruces Health Research Institute, University of the Basque Country, Leioa, Spain.
Reprints: María-Celia Morales, BioCruces Health Research Institute, Cruces University Hospital, 48903 Barakaldo, Vizcaya, Spain (e-mail: firstname.lastname@example.org).
The authors have no conflicts of interest to disclose.
Supported by grants from the University of the Basque Country/Instituto Clínico-Quirúrgico de Oftalmología (UE09/12), from the Saiotek Program of the Basque Government (S-PE10UN60), and from the University of the Basque Country (UFI11/44). V. Freire was supported by a fellowship from Torres-Quevedo Program.
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Received November 10, 2013
Accepted February 09, 2014