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Clinical and Histopathological Outcomes of Subconjunctival Triamcinolone Injection for the Treatment of Acute Ocular Alkali Burn in Rabbits

Saud, Esper E MD, MSc*†; Moraes, Haroldo V Jr MD, PhD*; Marculino, Leonardo G C MD‡; Gomes, José Alvaro P MD, PhD‡; Allodi, Silvana MSc, PhD†§; Miguel, Nádia C O MSc, PhD†

doi: 10.1097/ICO.0b013e318221ce99
Basic Investigation

Purpose: To evaluate the efficacy and safety of subconjunctival injection of triamcinolone in the treatment of acute ocular alkali burn in rabbits.

Methods: Two groups of 5 rabbits were subjected to alkali burn (1 N NaOH). One group was treated with 1 subconjunctival injection of 0.3 mL of triamcinolone and the other with 1 subconjunctival injection of 0.3 mL of 0.9% saline. The affected corneas were observed for vascularization and opacity approximately 10 minutes after the burn and also after 7, 14, and 21 days. Photographs were taken for observation and statistical analyses. At all time intervals, the corneas were classified according to predetermined scores. Twenty-one days after the treatment, the animals were anesthetized, and their eyes were enucleated and processed for histopathology.

Results: Greater vascularization and opacity appeared in the animals that were treated with saline than in those treated with subconjunctival triamcinolone (vascularization: 7 days, P = 0.0107; 14 days, P = 0.0099; and 21 days, P = 0.0088; opacity: 7 days, P = 0.0079; 14 days, P = 0.0112; and 21 days, P = 0.0255). These results were also compatible with the morphological and statistical analyses, which revealed a more intense inflammatory process in the group treated with saline (P = 0.0317). No complications, such as corneal melting, perforation, or infection, were observed.

Conclusions: Subconjunctival injection of triamcinolone may be a therapeutic option for the treatment of acute ocular burn because it reduced the corneal inflammatory process, opacity, and vascularization, with no apparent clinical changes in the general state of the animal.

From the *Department of Ophthalmology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; †Program of Cell and Developmental Biology, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; ‡Cornea and External Disease Service, Department of Ophthalmology of the Federal University of São Paulo, São Paulo, Brazil; §Postgraduate Program of Morphological Sciences, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Received for publication May 12, 2010; revision received March 3, 2011; accepted April 25, 2011.

Supported by the CNPq and the FAPERJ.

The authors state that they have no proprietary interest in the products named in this article.

Reprints: Nádia C. O. Miguel, Program of Cell and Developmental Biology, Institute of Biomedical Sciences, Av. Carlos Chagas Filho 373, Bloco F2-012, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil CEP: 21949-902 (e-mail: miguelnco@histo.ufrj.br).

© 2012 Lippincott Williams & Wilkins, Inc.