Skip Navigation LinksHome > March 2010 - Volume 29 - Issue 3 > Corneal Epithelial MT1-MMP Inhibits Vascular Endothelial Cel...
doi: 10.1097/ICO.0b013e3181b1165d
Basic Investigation

Corneal Epithelial MT1-MMP Inhibits Vascular Endothelial Cell Proliferation and Migration

Azar, Dimitri T MD*†‡; Casanova, Fabio H MD†‡; Mimura, Tatsuya MD, PhD*†; Jain, Sandeep MD*‡; Zhou, Zhongjun PhD§; Han, Kyu Yeon PhD*; Chang, Jin-Hong PhD*†‡

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Purpose: To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation.

Methods: We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed.

Results: Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect.

Conclusions: Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain.

© 2010 Lippincott Williams & Wilkins, Inc.


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