You could be reading the full-text of this article now if you...

If you have access to this article through your institution,
you can view this article in

Role of Extracellular Lumican in Corneal Stromal Infection

Hayashi, Yasuhito MD, PhD*†; Ohashi, Yuichi MD, PhD*

doi: 10.1097/ICO.0b013e3181ae9a68

Purpose: To explore the role of lumican in polymorphonuclear neutrophil granulocyte (PMN) migration in corneal stroma after bacterial infection.

Methods: Lumican wild-type (Lum+/+) green fluorescent protein (GFP)-positive PMNs were transplanted into lumican knockout (Lum−/) and heterozygous control (Lum+/) mice followed by corneal stromal injection of Staphylococcus aureus conjugated with pH-sensitive dye that expresses fluorescein when trapped in lysosomes.

Results: In Lum+/ corneal stroma, many small triangular or spindle-shaped cells labeled by pH-sensitive dye appeared within 10 minutes after S. aureus injection, whereas in Lum−/ corneal stroma, large cells or large cell complexes (morphologically, macrophages and macrophage-derived lymphatic vessels) appeared. Transplanted GFP-positive PMNs appeared in Lum+/ corneal stroma within 1 hour after S. aureus injection and were concentrated in the central injection area at 2 hours after injection. In Lum−/, on the other hand, relatively few GFP-positive cells were observed in corneal stroma even ≤ 24 hours after injection of bacteria.

Conclusions: Extracellular lumican is required for PMN migration in corneal stroma. In lumican null mouse cornea, macrophages and macrophage-derived lymphatic vessels act as first defense against staphylococcal infection.

Author Information

From the *Department of Ophthalmology; and †Regenerative Medicine, Ehime University School of Medicine, Ehime, Japan.

Supported by the Japanese Ministry of Education, Culture, Sports, Science and Technology (No. 19592024).

Reprints: Yasuhito Hayashi, MD, PhD, Department of Ophthalmology, Ehime University School of Medicine, 454 Shitsukawa, Toon, Ehime 791-0295, Japan (e-mail:

© 2009 Lippincott Williams & Wilkins, Inc.