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Cornea:
Basic Investigations

How Long Can Donor Sclera Be Safely Stored?

Romanchuk, K. G. MD, FRCSC; Nair, P. MD, BSc; Grahn, B. DVM

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Abstract

Purpose. To determine whether after prolonged storage of sclera in glycerin, there is any bacteriologic contamination that will reactivate, whether reconstituted sclera retains its tensile strength, and whether sclera retains its microstructural integrity.

Methods. Sixty-six scleral shells stored in glycerin for 9 to 19 years, as well as 11 controls stored for 6 months to 4 years, were studied by cutting a small wedge of tissue from the anterior margin of each and directly inoculating into thioglycolate broth, cutting an equatorial ring and determining its break strength using a tensiometer, and cutting a small piece from the remaining posterior portion and examining by scanning electron microscopy.

Results. After such prolonged storage, bacteriologic contamination was not detected, tensile strength generally increased with increasing duration of storage, and ultrastructural integrity was maintained on scanning electron microscopy.

Conclusions. This study suggests that storage of scleral shells can be safely prolonged; we hope this can facilitate an increased supply of donated sclera to patients and surgeons.

We have had a few unused scleral shells per year since the inception of our eye bank in 1981, and these were quarantined and stored separately. This tissue was not otherwise suitable for clinical use because of prolonged storage (with some specimens predating mandatory serologic testing of donors!) and had been donated for transplant, teaching, or research.

We studied 66 scleral shells donated between 1981 and 1991 and stored in glycerin for 9 to 19 years, as well as 11 scleral shells more recently donated and stored between 6 months and 4 years, to answer three questions: (1) Is there any bacteriologic contamination that will reactivate? (2) When reconstituted (in balanced salt solution) does sclera retain its tensile strength after such prolonged storage? (3) Does sclera retain its microstructural integrity with such prolonged storage, as determined by scanning electron microscopy?

© 2003 Lippincott Williams & Wilkins, Inc.

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