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Human Corneal Storage in Modified McCarey-Kaufman and K-Sol Media: Effect on Endothelial Na+/K+ ATPase Pump Site Density and Permeability.

McDermott, M. L. M.D.; Watsky, M. A. Ph.D.; Geroski, D. H. Ph.D.; Edelhauser, H. F. Ph.D.

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: Endothelial permeability (Pac) to carboxyfluorescein and Na+/K+ ATPase pump site density were determined in human corneas following storage for 4 or 7 days at 4[degrees]C in either modified McCarey-Kaufman (mMK) or K-Sol media. Following 4 days of storage, Pac values for mMK-and K-Sol-preserved corneas were not significantly different from those of their prestorage mates. After 7 days of storage, however, corneas stored in K-Sol media showed a significant increase in Pac compared to their prestorage mates, whereas the mMK-stored corneas showed no change in Pac. Na+/K+ ATPase pump site density determined using [3H]ouabain was similar to a control group in the K-Sol-stored tissue but higher in the mMK-stored tissue following 7 days of storage. These studies suggest that mMK medium maintains endothelial barrier function and Na+/K+ ATPase pump site density at least as well as K-Sol medium through 7 days of corneal storage.

(C) Lippincott-Raven Publishers.


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