Current Opinion in Organ Transplantation:
STEM CELL TRANSPLANTATION: Edited by Elke Eggenhofer
Mesenchymal stromal cells to promote solid organ transplantation tolerance
Casiraghi, Federica; Perico, Norberto; Remuzzi, Giuseppe
Department of Immunology and Transplantation, Transplant Research Center ‘Chiara Cucchi de Alessandri e Gilberto Crespi’, Mario Negri Institute for Pharmacological Research, Ospedali Riuniti – ‘Mario Negri’ Institute for Pharmacological Research, Bergamo, Italy
Correspondence to Federica Casiraghi, Transplant Research Center, ‘Chiara Cucchi de Alessandri e Gilberto Crespi’, Mario Negri Institute for Pharmacological Research, Via Camozzi 3, 24020 Ranica (Bergamo), Italy. Tel: +39 035 4535361; fax: +39 035 4535377; e-mail: firstname.lastname@example.org
Purpose of review: Mesenchymal stromal cells (MSCs) possess unique immunomodulatory features. MSCs dampen effector T-cell response while promoting the emergence of regulatory T cells. By skewing this balance, MSC could represent the ideal strategy for tolerance induction in organ transplantation. Here we review recent evidence on the efficacy of MSC-based therapy in experimental models of solid organ transplantation as well as the early clinical experiences in kidney transplantation.
Recent findings: MSC infusion in experimental models of solid organ transplantation resulted in a Treg-mediated tolerance. MSC also synergized with low-dose or transient pharmacological immunosuppression in inducing long-term graft survival indicating that these cells could allow safe minimization of maintenance drug therapy. Early results from clinical studies in kidney transplant recipients reported encouraging results on the immunoregulatory effect of MSC, although posttransplant MSC infusion could associate with acute graft dysfunction (engraftment syndrome).
Summary: Immunoregulatory functions of MSC are not fixed but rather the result of microenvironment they encounter in vivo. Further studies are needed to establish how and wherein these cells have to be administered and how they may function to safely modulate host immune response in vivo in clinical transplant setting.
Since the first successful renal transplantation in Boston in 1954 , organ transplantation has made dramatic strides, evolving from an experimental procedure to standard of care in the treatment of patients with end-stage organ disease. Although powerful immunosuppressive drugs are undoubtedly the cornerstone of transplant success by preventing acute cellular rejection , they affect the function of all responding T cells irrespective of their antigen-specificity, rendering transplant recipients susceptible to life-threatening infections and malignancy [3,4]. In addition, life-long use of broad-spectrum pharmacological immunosuppression is associated with unwanted side effects, including accelerated cardiovascular disease, metabolic complications and with a direct toxic effect to transplanted tissues [3,4], eventually contributing to long-term graft loss, a common event in renal transplantation. Ideally, the induction of donor-specific tolerance would overcome these shortcomings, possibly allowing indefinite graft survival .
The immune system has evolved multiple mechanisms for controlling the effector adaptive immune response . Transplantation of a major histocompatibility complex-incompatible graft triggers the activation of graft destructive effector T cells as well as protective regulatory T cells (Tregs); it is the balance of such opposing subsets that ultimately determines the fate of the allotransplant . The most extensively studied populations of Tregs are the so-called naturally occurring CD4+CD25+Foxp3+ Treg that develop in the thymus [7,8] and the adaptive Tregs that are induced in the periphery in response to antigen stimulation under tolerogenic conditions . Together, Tregs maintain tolerance to self-antigens and control excessive immune response to foreign antigens and may contribute to the induction and maintenance of tolerance to allografts [10,11].
Bone marrow-derived multipotent mesenchymal stromal cells (MSC) have emerged as a promising cell population for immunomodulatory therapy in transplantation given their unique immunoregulatory properties on both the adaptive  and innate [13▪▪] immune cells. MSC are capable of suppressing T effector cells  including memory T cells [15,16], skewing T cells toward Foxp3+ Tregs with concurrent suppression of Th1, Th2 or Th17 responses . The findings that MSC target effector/memory T cells and promote the development of Tregs have led to propose MSC as a novel, potentially suitable cell-based approach for tolerance induction in organ transplantation.
Here, we have reviewed recent evidence on the capability of MSC to skew the balance between T effector cells and Tregs as well as the safety and efficacy of MSC-based therapy in experimental models of solid organ transplantation and in early clinical experience.
MESENCHYMAL STROMAL CELLS AND REGULATORY T CELL GENERATION
MSC are a heterogeneous population of adult, fibroblast-like multipotent cells characterized by their ability to differentiate into tissues of mesodermal lineages, including adipocytes, chondrocytes and osteocytes . First identified and isolated from the bone marrow as plastic adherent cells , MSC are now isolated from a number of other sources including umbilical cord blood, adipose tissue and muscle [19,20]. The isolation of MSC by in-vitro expansion of plastic-adherent cells yields a heterogeneous cell population evidenced by the different morphology and functional potential. In order to create a consensus and more uniformly characterize these cells the International Society of Cellular Therapy proposed a standard set to define the identity of MSC : adherence to plastic surfaces; potential to differentiate into osteocytes, adipocytes and chondrocytes under standard in-vitro differentiating conditions; and expression of CD105, CD73 and CD90 and must lack expression of CD45, CD34, CD14, CD11b, CD79a and HLA-DR.
Several in-vitro and in-vivo studies have documented the remarkable ability of MSC to polarize T cells toward a regulatory phenotype. In-vitro coincubation of human MSC with peripheral blood mononuclear cells or with purified CD4+ T cells induced the differentiation of CD4+ T cells into Foxp3-expressing Tregs [22–25], a process involving direct MSC contact with T cells followed by prostaglandin E2 and transforming growth factor β-1 (TGFβ-1) expression [22,24]. Expanded Tregs potently suppressed the alloantigen-specific proliferative response in mixed-lymphocyte reaction (MLR) assay [23,24]. MSC induced a Treg phenotype (CD25brightFoxp3+CD127low) both in naive CD3+CD45RA+ and in memory CD3+CD45RO+ T cells . Other potential mechanisms of MSC-induced Treg generation include the release of soluble HLA-G5, a nonclassical HLA class I molecule  or of microvescicles . MSC are also able to reprogram fully differentiated Th17 cells into Foxp3-expressing Tregs . However, both the activation state of CD4+ T cells and the cytokine milieu that MSC encounter dictate the ultimate cell outcome. Whereas the early addition of MSC to T cells cultured under Th1 and Th17 polarizing conditions exerted an extensive suppressive effect on all CD4+ T-cell lineages, MSC added to already differentiated Th1/Th17 cells decreased IFNγ production by Th1 cells, but paradoxically increased proinflammatory interleukin 17 (IL-17) [30▪]. Moreover, MSC cultured in the presence of inflammatory cytokines secreted significant levels of IL-6, which, in addition to a spontaneous production of TGFβ supported retinoic acid-related orphan receptor γt expression and development of Th17 [31▪].
By exerting inhibitory effects on antigen presenting cells (APC), MSC can generate regulatory APC with own Treg promoting activity. Dendritic cells cultured in the presence of MSC or conditioned medium expressed lower level of costimulatory molecules, hardly stimulated T-cell proliferation and efficiently generated Tregs through the release of TGFβ [32,33]. Tregs could also be expanded by macrophages polarized by MSC toward the M2 anti-inflammatory phenotype [34▪]. In the in-vitro setting of anti-CD3/anti-CD28 antibody T-cell stimulation, MSC promoted the differentiation of the monocyte fraction of peripheral blood mononuclear cells into IL-10- secreting M2 immunosuppressive macrophages via the induction of indoleamine 2,3-dioxygenase expression [35▪]. These macrophages were in turn implicated in the generation of Tregs [35▪].
The role of macrophages in MSC-induced Tregs has been recently confirmed in vivo in mouse models of fibrillin-mutated systemic sclerosis and experimental colitis [36▪▪]. Indeed, systemic infusion of either syngeneic or allogeneic murine bone marrow MSC in these mice-induced transient T-cell apoptosis via the FasL–Fas pathway, which triggered macrophages to produce high levels of TGFβ in the peripheral blood, eventually enhancing CD4+CD25+Foxp3+ Treg generation. This effect translated into the amelioration of the disease phenotypes [36▪▪].
The polarization of T cells toward a Treg phenotype with a concomitant decrease in Th1/Th17 development has been also shown to be associated with MSC immunomodulatory effect in other experimental models of autoimmune and inflammatory diseases such as systemic lupus erythematosus , collagen-induced arthritis [38–40], diabetes [41–44], colitis  and autoimmune myasthenia gravis [46,47].
Together these in-vitro and in-vivo studies indicate the ability of MSC to modulate the immune response to antigens mainly by promoting the generation of T cells with regulatory phenotype and possibly lowering the availability of Th1/Th17 effector cells.
MESENCHYMAL STROMAL CELLS IN EXPERIMENTAL MODELS OF SOLID ORGAN TRANSPLANTATION
Almost a decade has elapsed since the first study reporting the capability of MSC to prolong survival of skin graft in nonhuman primates . Subsequent studies in rodent models of heart [49–55], liver  islet [57–59,60▪,61▪], kidney [62,63▪] and composite tissue [64▪,65▪] allotransplantation confirmed the immunomodulatory potential of MSC in transplantation (Table 1[48–59,60▪,61▪,62,63▪–65▪,66]). Of note, long-term graft acceptance achieved after MSC infusion alone or in association with low-dose immunosuppressive drugs was found to be related to the expansion of Tregs [52,53,56,60▪,61▪,62,63▪–65▪] or tolerogenic dendritic cells [51,53].
There is also evidence that Treg depletion abrogated the MSC effect of inducing long-term graft acceptance [62,63▪], highlighting that MSC-mediated tolerance is maintained by Tregs. Regulatory T-cells isolated from long-term survival mice were antigen-specific .
We recently demonstrated that the timing of MSC infusion in respect to solid organ transplantation is one of the main factors affecting MSC capability to expand Tregs and prolong graft survival [63▪]. Murine MSC given to mice pretransplantation localized preferentially into lymphoid organs where allowed early expansion of Tregs, eventually leading to immune tolerance to subsequent kidney allografts. At variance, MSC infused posttransplant localized preferentially into the kidney graft with very low expansion of Tregs [63▪]. Intragraft MSC localization associated with acute graft dysfunction, intragraft neutrophil recruitment and C3 deposition and poor graft survival [63▪]. Similarly, the migration of MSC into recipient lymphoid tissues have been shown to be critical for MSC immunomodulatory effects in autoimmune encephalomyelitis , autoimmune enteropathy , diabetes [69▪] and graft-versus-host disease , supporting the concept that MSC need to interact with immune cells in sites of initial effector T-cell priming in order to effectively exert immunomodulation.
Most of the experimental studies with MSC in organ transplantation have been performed without any additional pharmacological immunosuppressive therapy. However, in the perspective of translating cell-based MSC therapy to clinical transplant programs, it is critical to evaluate the possible negative impact of currently used antirejection drugs on MSC-induced Treg generation and function and eventually graft survival.
Data on the effect of cyclosporine (CsA) on MSC-induced immunoregulation are controversial [55,66] (Table 1). There is experimental and clinical evidence that calcineurin inhibitors (CNI), by blocking IL-2 expression in T cells, prevent both Treg development and homeostasis , although at low-dose these drugs may expand Tregs in both the periphery and the allografts .
In a mouse model of in-vivo MLR, CsA inhibited the MSC-mediated suppression of CD4+ T-cell proliferation . At variance, other in-vitro studies have documented the CsA did not interfere with MSC-mediated Treg generation  and that MSC synergized with CsA in inhibiting T lymphocyte activity . The combination of MSC and subtherapeutic doses of CsA exerted a synergistic immunosuppressive effect, which translated into long-term graft acceptance of islet allografts [58,60▪]. In rat islet allograft models MSC and low-dose CsA induced early expansion of IL-10 producing CD11b cells, which mediated T-cell hyporesponsiveness and allowed long-term Foxp3 Tregs expansion in lymph nodes and in the graft [60▪]. Moreover, in swine the combination of multiple infusions of allogeneic MSC with short-term CsA immunosuppression achieved indefinite graft survival of hind-limb transplants [64▪] and prolonged the survival of a hemi-facial transplant [65▪]. In both studies long-term surviving animals showed increased levels of Foxp3 Tregs in the periphery and in the graft [64▪,65▪].
On the contrary, mammalian target-of-rapamycin inhibitors have been consistently shown to sustain Treg expansion in vitro and in vivo in animal models and kidney transplant recipients . In an experimental model of heart transplantation in mice rapamycin synergized with MSC in inducing Treg-mediated tolerance . Similarly, in the same model in rats, mycophenolate combined with donor MSC induced long-term graft acceptance [51,54].
Altogether these results indicate that in experimental models MSC infusion synergized with low-dose or transient immunosuppressive drug treatment in inducing long-term graft acceptance, indicating that these cells allow safe minimization of maintenance pharmacological antirejection therapy.
MESENCHYMAL STROMAL CELLS IN KIDNEY TRANSPLANTATION IN HUMANS
There are few protocols of MSC-based therapy in organ transplantation (http://www.clinicaltrials.gov). Actually, clinical trials on the use of MSC in kidney and liver transplantation are being performed in our center in Bergamo, Italy (NCT00752479), in Leiden, The Netherlands (NCT00734396), in Liege, Belgium (NCT01429038) and in China (NCT00659620). So far only results from the Italian and Chinese experiences with MSC in living-donor kidney transplant recipients have been published. Our protocol is aimed at characterizing the safety and tolerability of peritransplant MSC infusion and to verify whether MSC, by skewing Treg/Teff balance allow creating a protolerogenic environment. We initially started with two living-related donor kidney recipients who were given ex-vivo expanded, autologous, bone marrow-derived MSC at day 7 posttransplant, after induction therapy with basiliximab/low-dose thymoglobulin [75▪]. MSC infusion did promote on long-term a protolerogenic environment characterized by lower memory/effector CD8+ T cells, expansion of CD4+ Tregs and reduction of donor-specific CD8+ T-cell cytotoxicity, compared with control kidney transplant recipients given the same induction therapy but not MSC. However, few days after cell infusion, both MSC-treated patients developed acute renal insufficiency. Histological and immunohistochemical analysis of graft infiltrating cells did exclude an acute cellular or humoral rejection, but intragraft recruitment of neutrophils together with MSC, as well as complement-C3 deposition were observed [75▪].
It was hypothesized that the subclinical inflammatory environment of the graft in the few days postsurgery could have favoured the prevalent intragraft recruitment and activation of the infused MSC promoting a proinflammatory milieu with eventual acute renal dysfunction (engraftment syndrome), as reported by others with combined kidney and bone marrow transplantation . This hypothesis has been confirmed back into a murine kidney transplant model showing that MSC administration before (day-1) but not few days after kidney transplantation avoided the acute deterioration of graft function, while maintaining the immunomodulatory effect of MSC [63▪].
The Chinese group performed a single-site prospective, randomized study aimed at comparing the risk-benefit profile of bone marrow-derived autologous MSC infusion (at kidney reperfusion and 2 weeks later) versus induction therapy with the anti-IL-2 receptor antibody basiliximab in living-related donor kidney transplants [77▪]. MSC treatment resulted in lower incidence of acute rejection at 6 months posttransplant, decreased risk of opportunistic infection and better estimated renal function. The investigators concluded that MSC may replace basiliximab induction therapy, allowing the use of lower than conventional CNI maintenance doses without compromising patient safety and graft outcome. However, lower acute rejection rate and better renal function documented at 6 months after transplantation were transient and not confirmed at 1 year. The study has been criticized in a recent letter . Unfortunately, this study did not report any attempt to in-depth assess the in-vivo effects of MSC on host immune system, especially on Treg and effector T-cell function by any immunological tests, which are mandatory for an innovative cell therapy still in its infancy before moving it to routine clinical application for transplant programs.
Cell therapy with MSC in solid organ transplantation has undoubtedly a great potential. However, although initial preclinical and early clinical results appear promising, moving the concept of MSC-based therapy forward toward clinical application should be critically assessed. We have to be aware that, so far, our knowledge about MSC is too scarce for embarking in large clinical trials and there remain many open questions both on the risk and the real benefit of these cells. Further studies are needed to establish how and where these cells have to be administered and how they may function to modulate host immune response in vivo in clinical transplant setting.
Rather than studying thousands of patients without enough attempt to safety issues and mechanistic/immunomodulatory pathways it seems preferable in our opinion in this kind of studies to proceed in few patients, however, very intensively investigated. Issues like source, dose, timing of administration, in-vivo localization, interaction with immunosuppressive drugs, whether these cells have to be used for prevention of acute rejection or for tolerance induction have not been addressed in this field and more explorative studies are required before embarking in formal clinical trials.
The Authors are member of the Mesenchymal Stem Cells in Solid Organ Transplantation (MISOT) study group, http://www.misot.de.
Conflicts of interest
This study has been partially supported by grants from Fondazione ART per la Ricerca sui Trapianti (Milan, Italy).
Grant support was received from Superpig Program, project co-financed by Lombardy Region through the ‘Fund for promoting institutional agreements’
The authors of this manuscript have no conflict of interest to disclose.
REFERENCES AND RECOMMENDED READING
Papers of particular interest, published within the annual period of review, have been highlighted as:
▪ of special interest
▪▪ of outstanding interest
Additional references related to this topic can also be found in the Current World Literature section in this issue (p. 116).
1. Murray JE, Merrill JP, Harrison JH. Kidney transplantation between seven pairs of identical twins. Ann Surg 1958; 148:343–359.
2. U.S. Department of Health and Human Services 2008 Annual report of the U.S. Organ Procurement and Transplantation Network and the Scientific Registry of Transplant Recipients: Transplant Data. U.S. Department of Health and Human Services, Health Resources and Services Administration, Healthcare System Bureau, division of Transplantation, Rockville, MD; 1998–2007. http://www.str.org
3. Lodhi SA, Lamb KE, Meier-Kriesche HU. Solid organ allograft survival improvement in the United States: the long-term does not mirror the dramatic short-term success. Am J Transplant 2011; 11:1226–1235.
4. Meier-Kriesche HU, Schold JD, Srinivas TR, Kaplan B. Lack of improvement in renal allograft survival despite a marked decrease in acute rejection rates over the most recent era. Am J Transplant 2004; 4:378–383.
5. Sayegh MH, Remuzzi G. Clinical update: immunosuppression minimisation. Lancet 2007; 369:1676–1678.
6. Ingulli E. Mechanism of cellular rejection in transplantation. Pediatr Nephrol 2010; 25:61–74.
7. Sakaguchi S. Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in immunological tolerance to self and nonself. Nat Immunol 2005; 6:345–352.
8. Fontenot JD, Rudensky AY. A well adapted regulatory contrivance: regulatory T cell development and the forkhead family transcription factor Foxp3. Nat Immunol 2005; 6:331–337.
9. Curotto de Lafaille MA, Lafaille JJ. Natural and adaptive foxp3+ regulatory T cells: more of the same or a division of labor? Immunity 2009; 30:626–635.
10. Wood KJ, Sakaguchi S. Regulatory T cells in transplantation tolerance. Nat Rev Immunol 2003; 3:199–210.
11. Li XC, Turka LA. An update on regulatory T cells in transplant tolerance and rejection. Nat Rev Nephrol 2010; 6:577–583.
12. Griffin MD, Ritter T, Mahon BP. Immunological aspects of allogeneic mesenchymal stem cell therapies. Hum Gene Ther 2010; 21:1641–1655.
13▪▪. Le Blanc K, Mougiakakos D. Multipotent mesenchymal stromal cells and the innate immune system. Nat Rev Immunol 2012; 12:383–396.
An outstanding review of current knowledge regarding the effects of MSCs on the various components of the innate immune system and vice versa.
14. Duffy MM, Ritter T, Ceredig R, Griffin MD. Mesenchymal stem cell effects on T-cell effector pathways. Stem Cell Res Ther 2011; 2:34.
15. Krampera M, Glennie S, Dyson J, et al. Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigen-specific T cells to their cognate peptide. Blood 2003; 101:3722–3729.
16. Karlsson H, Samarasinghe S, Ball LM, et al. Mesenchymal stem cells exert differential effects on alloantigen and virus-specific T-cell responses. Blood 2008; 112:532–541.
17. Caplan AI. Mesenchymal stem cells. J Orthop Res 1991; 9:641–650.
18. Friedenstein AJ, Chailakhyan RK, Latsinik NV, et al. Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues. Cloning in vitro and retransplantation in vivo. Transplantation 1974; 17:331–340.
19. da Silva Meirelles L, Chagastelles PC, Nardi NB. Mesenchymal stem cells reside in virtually all postnatal organs and tissues. J Cell Sci 2006; 119:2204–2213.
20. Kern S, Eichler H, Stoeve J, et al. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 2006; 24:1294–1301.
21. Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006; 8:315–317.
22. Aggarwal S, Pittenger MF. Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood 2005; 105:1815–1822.
23. Prevosto C, Zancolli M, Canevali P, et al. Generation of CD4+ or CD8+ regulatory T cells upon mesenchymal stem cell-lymphocyte interaction. Haematologica 2007; 92:881–888.
24. English K, Ryan JM, Tobin L, et al. Cell contact, prostaglandin E(2) and transforming growth factor beta 1 play nonredundant roles in human mesenchymal stem cell induction of CD4+CD25(High) forkhead box P3+ regulatory T cells. Clin Exp Immunol 2009; 156:149–160.
25. Maccario R, Podesta M, Moretta A, et al. Interaction of human mesenchymal stem cells with cells involved in alloantigen-specific immune response favors the differentiation of CD4+ T-cell subsets expressing a regulatory/suppressive phenotype. Haematologica 2005; 90:516–525.
26. Di Ianni M, Del Papa B, De Ioanni M, et al. Mesenchymal cells recruit and regulate T regulatory cells. Exp Hematol 2008; 36:309–318.
27. Selmani Z, Naji A, Zidi I, et al. Human leukocyte antigen-G5 secretion by human mesenchymal stem cells is required to suppress T lymphocyte and natural killer function and to induce CD4+CD25highFOXP3+ regulatory T cells. Stem Cells 2008; 26:212–222.
28. Mokarizadeh A, Delirezh N, Morshedi A, et al. Microvesicles derived from mesenchymal stem cells: Potent organelles for induction of tolerogenic signaling. Immunol Lett 2012; 147:47–54.
29. Ghannam S, Pene J, Torcy-Moquet G, et al. Mesenchymal stem cells inhibit human Th17 cell differentiation and function and induce a T regulatory cell phenotype. J Immunol 2010; 185:302–312.
30▪. Carrion F, Nova E, Luz P, et al. Opposing effect of mesenchymal stem cells on Th1 and Th17 cell polarization according to the state of CD4+ T cell activation. Immunol Lett 2011; 135:10–16.
The study showed how the activation status of T cells as well as the cytokine milieu that MSC encounter dictate the effect of MSC on Th17 cells.
31▪. Svobodova E, Krulova M, Zajicova A, et al. The role of mouse mesenchymal stem cells in differentiation of naive T-cells into anti-inflammatory regulatory T-cell or proinflammatory helper T-cell 17 population. Stem Cells Dev 2012; 21:901–910.
These studies showed how the activation status of T cells as well as the cytokine milieu that MSC encounter dictate the effect of MSC on Th17 cells.
32. Zhang B, Liu R, Shi D, et al. Mesenchymal stem cells induce mature dendritic cells into a novel Jagged-2-dependent regulatory dendritic cell population. Blood 2009; 113:46–57.
33. Zhao ZG, Xu W, Sun L, et al. Immunomodulatory function of regulatory dendritic cells induced by mesenchymal stem cells. Immunol Invest 2012; 41:183–198.
34▪. Ylostalo JH, Bartosh TJ, Coble K, Prockop DJ. Human mesenchymal stem/stromal cells (hMSCs) cultured as spheroids are self-activated to produce prostaglandin E2 (PGE2) that directs stimulated macrophages into an anti-inflammatory phenotype. 2012. Stem Cells 2012; 30:2283–2296.
The study describes how MSC induce macrophages to differentiate toward an anti-inflammatory M2 phenotype.
35▪. Francois M, Romieu-Mourez R, Li M, Galipeau J. Human MSC suppression correlates with cytokine induction of indoleamine 2,3-dioxygenase and bystander M2 macrophage differentiation. Mol Ther 2012; 20:187–195.
These studies describe how MSC induce macrophages to differentiate toward an anti-inflammatory M2 phenotype.
36▪▪. Akiyama K, Chen C, Wang D, Xu X, et al. Mesenchymal-stem-cell-induced immunoregulation involves FAS-ligand-/FAS-mediated T cell apoptosis. Cell Stem Cell 2012; 10:544–555.
This study uncovers a previously unrecognized MSC-mediated therapeutic mechanism by which MSC use FAS to regulate MCP-1 secretion for T-cell recruitment and subsequent use FAS to induce T-cell apoptosis. Macrophages subsequently take the apoptotic debris to release TGFβ, leading to upregulation of Tregs and, ultimately, immune tolerance.
37. Sun L, Akiyama K, Zhang H, et al. Mesenchymal stem cell transplantation reverses multiorgan dysfunction in systemic lupus erythematosus mice and humans. Stem Cells 2009; 27:1421–1432.
38. Bouffi C, Bony C, Courties G, et al. IL-6-dependent PGE2 secretion by mesenchymal stem cells inhibits local inflammation in experimental arthritis. PLoS One 2010; 5:e14247.
39. Park MJ, Park HS, Cho ML, et al. Transforming growth factor beta-transduced mesenchymal stem cells ameliorate experimental autoimmune arthritis through reciprocal regulation of Treg/Th17 cells and osteoclastogenesis. Arthritis Rheum 2011; 63:1668–1680.
40. Zhou B, Yuan J, Zhou Y, et al. Administering human adipose-derived mesenchymal stem cells to prevent and treat experimental arthritis. Clin Immunol 2011; 141:328–337.
41. Abdi R, Fiorina P, Adra CN, et al. Immunomodulation by mesenchymal stem cells: a potential therapeutic strategy for type 1 diabetes. Diabetes 2008; 57:1759–1767.
42. Zhao W, Wang Y, Wang D, et al. TGF-beta expression by allogeneic bone marrow stromal cells ameliorates diabetes in NOD mice through modulating the distribution of CD4+ T cell subsets. Cell Immunol 2008; 253:23–30.
43. Madec AM, Mallone R, Afonso G, et al. Mesenchymal stem cells protect NOD mice from diabetes by inducing regulatory T cells. Diabetologia 2009; 52:1391–1399.
44. Bassi EJ, Mallone PM, Moreira Sa CS, et al. Immune regulatory properties of allogeneic adipose-derived mesenchymal stem cells in the treatment of experimental autoimmune diabetes. Diabetes 2012; 61:2534–2545.
45. Gonzalez MA, Gonzalez-Rey E, Rico L, et al. Adipose-derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses. Gastroenterology 2009; 136:978–989.
46. Kong QF, Sun B, Bai SS, et al. Administration of bone marrow stromal cells ameliorates experimental autoimmune myasthenia gravis by altering the balance of Th1/Th2/Th17/Treg cell subsets through the secretion of TGF-beta. J Neuroimmunol 2009; 207:83–91.
47. Kong QF, Sun B, Wang GY, et al. BM stromal cells ameliorate experimental autoimmune myasthenia gravis by altering the balance of Th cells through the secretion of IDO. Eur J Immunol 2009; 39:800–809.
48. Bartholomew A, Sturgeon C, Siatskas M, et al. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo. Exp Hematol 2002; 30:42–48.
49. Zhou HP, Yi DH, Yu SQ, et al. Administration of donor-derived mesenchymal stem cells can prolong the survival of rat cardiac allograft. Transplant Proc 2006; 38:3046–3051.
50. Chabannes D, Hill M, Merieau E, et al. A role for heme oxygenase-1 in the immunosuppressive effect of adult rat and human mesenchymal stem cells. Blood 2007; 110:3691–3694.
51. Popp FC, Eggenhofer E, Renner P, et al. Mesenchymal stem cells can induce long-term acceptance of solid organ allografts in synergy with low-dose mycophenolate. Transpl Immunol 2008; 20:55–60.
52. Casiraghi F, Azzollini N, Cassis P, et al. Pretransplant infusion of mesenchymal stem cells prolongs the survival of a semiallogeneic heart transplant through the generation of regulatory T cells. J Immunol 2008; 181:3933–3946.
53. Ge W, Jiang J, Baroja ML, et al. Infusion of mesenchymal stem cells and rapamycin synergize to attenuate alloimmune responses and promote cardiac allograft tolerance. Am J Transplant 2009; 9:1760–1772.
54. Eggenhofer E, Renner P, Soeder Y, et al. Features of synergism between mesenchymal stem cells and immunosuppressive drugs in a murine heart transplantation model. Transpl Immunol 2011; 25:141–147.
55. Inoue S, Popp FC, Koehl GE, et al. Immunomodulatory effects of mesenchymal stem cells in a rat organ transplant model. Transplantation 2006; 81:1589–1595.
56. Wang Y, Zhang A, Ye Z, et al. Bone marrow-derived mesenchymal stem cells inhibit acute rejection of rat liver allografts in association with regulatory T-cell expansion. Transplant Proc 2009; 41:4352–4356.
57. Ding Y, Xu D, Feng G, et al. Mesenchymal stem cells prevent the rejection of fully allogeneic islet grafts by the immunosuppressive activity of matrix metalloproteinase-2 and -9. Diabetes 2009; 58:1797–1806.
58. Solari MG, Srinivasan S, Boumaza I, et al. Marginal mass islet transplantation with autologous mesenchymal stem cells promotes long-term islet allograft survival and sustained normoglycemia. J Autoimmun 2009; 32:116–124.
59. Li FR, Wang XG, Deng CY, et al. Immune modulation of co-transplantation mesenchymal stem cells with islet on T and dendritic cells. Clin Exp Immunol 2010; 161:357–363.
60▪. Kim YH, Wee YM, Choi MY, et al. Interleukin (IL)-10 induced by CD11b(+) cells and IL-10-activated regulatory T cells play a role in immune modulation of mesenchymal stem cells in rat islet allografts. Mol Med 2011; 17:697–708.
This study, together with [61▪], described how MSC administration delays rejection of allogeneic islets in rodents by inducing Foxp3-expressing Tregs in lymph nodes and grafts.
61▪. Xu DM, Yu XF, Zhang D, et al. Mesenchymal stem cells differentially mediate regulatory T cells and conventional effector T cells to protect fully allogeneic islet grafts in mice. Diabetologia 2012; 55:1091–1102.
This study, together with [60▪] described how MSC administration delays rejection of allogeneic islets in rodents by inducing Foxp3-expressing Tregs in lymph nodes and grafts.
62. Ge W, Jiang J, Arp J, et al. Regulatory T-cell generation and kidney allograft tolerance induced by mesenchymal stem cells associated with indoleamine 2,3-dioxygenase expression. Transplantation 2010; 90:1312–1320.
63▪. Casiraghi F, Azzollini N, Todeschini M, et al. Localization of mesenchymal stromal cells dictates their immune or proinflammatory effects in kidney transplantation. Am J Transplant 2012; 12:2373–2383.
This study shows that in a murine kidney transplant model, posttransplant MSC infusion causes premature graft dysfunction and fails to prolong graft survival, whereas pretransplant MSC infusion induces a significant prolongation of kidney graft survival by a regulatory T-cell dependent mechanism.
64▪. Kuo YR, Chen CC, Shih HS, et al. Prolongation of composite tissue allotransplant survival by treatment with bone marrow mesenchymal stem cells is correlated with T-cell regulation in a swine hind-limb model. Plast Reconstr Surg 2011; 127:569–579.
In composite tissue allotransplantation models in large animals the combination of allogeneic MSC with short-term immunosuppression prolonged graft survival and was associated with increased levels of Foxp3 Tregs in the periphery and in the graft.
65▪. Kuo YR, Chen CC, Goto S, et al. Immunomodulatory effects of bone marrow-derived mesenchymal stem cells in a swine hemi-facial allotransplantation model. PLoS One 2012; 7:e35459.
In composite tissue allotransplantation models in large animals the combination of allogeneic MSC with short-term immunosuppression prolonged graft survival and was associated with increased levels of Foxp3 Tregs in the periphery and in the graft.
66. Longoni B, Szilàgyi E, Puviani L, et al. Mesenchymal stem cell-based immunomodulation in allogeneic heterotopic heart-lung transplantation. J Transplant Technol Res 2012; 2:107.
67. Zappia E, Casazza S, Pedemonte E, et al. Mesenchymal stem cells ameliorate experimental autoimmune encephalomyelitis inducing T-cell anergy. Blood 2005; 106:1755–1761.
68. Parekkadan B, Tilles AW, Yarmush ML. Bone marrow-derived mesenchymal stem cells ameliorate autoimmune enteropathy independently of regulatory T cells. Stem Cells 2008; 26:1913–1919.
69▪. Ezquer F, Ezquer M, Contador D, et al. The antidiabetic effect of mesenchymal stem cells is unrelated to their transdifferentiation potential but to their capability to restore TH1/TH2 balance and to modify the pancreatic microenvironment. Stem Cells 2012; 30:1664–1674.
This study documents that the antidiabetic effect of MSC was correlated to their engraftment into secondary lymphoid organs associated with reduction of autoreactive T cells together with an increase in Treg cells.
70. Highfill SL, Kelly RM, O'Shaughnessy MJ, et al. Multipotent adult progenitor cells can suppress graft-versus-host disease via prostaglandin E2 synthesis and only if localized to sites of allopriming. Blood 2009; 114:693–701.
71. Zeiser R, Nguyen VH, Beilhack A, et al. Inhibition of CD4+CD25+ regulatory T-cell function by calcineurin-dependent interleukin-2 production. Blood 2006; 108:390–399.
72. Wang Z, Shi B, Jin H, et al. Low-dose of tacrolimus favors the induction of functional CD4+
regulatory T cells in solid-organ transplantation. Int Immunopharmacol 2009; 9:564–569.
73. Shi D, Liao L, Zhang B, et al. Human adipose tissue-derived mesenchymal stem cells facilitate the immunosuppressive effect of cyclosporin A on T lymphocytes through Jagged-1-mediated inhibition of NF-kappaB signaling. Exp Hematol 2011; 39:214–224.e211.
74. Battaglia M, Stabilini A, Roncarolo MG. Rapamycin selectively expands CD4+CD25+FoxP3+ regulatory T cells. Blood 2005; 105:4743–4748.
75▪. Perico N, Casiraghi F, Introna M, et al. Autologous mesenchymal stromal cells and kidney transplantation: a pilot study of safety and clinical feasibility. Clin J Am Soc Nephrol 2011; 6:412–422.
This is the first report on the immunomodulatory effect of MSC in living-related kidney transplant recipients documenting that peritransplant infusion induced acute kidney graft dysfunction.
76. Farris AB, Taheri D, Kawai T, et al. Acute renal endothelial injury during marrow recovery in a cohort of combined kidney and bone marrow allografts. Am J Transplant 2011; 11:1464–1477.
77▪. Tan J, Wu W, Xu X, et al. Induction therapy with autologous mesenchymal stem cells in living-related kidney transplants: a randomized controlled trial. JAMA 2012; 307:1169–1177.
This study reports results of a perspective, randomized study with autologous mesenchymal stem cells versus induction therapy with the anti IL-2 receptor antibody in living-related kidney transplants.
78. Riella LV, Chandraker A. Stem cell therapy in kidney transplantation. JAMA 2012; 308:130.
This article has been cited 2 time(s).
Clinical & Developmental ImmunologyTransplant Tolerance: New Insights and Strategies for Long-Term Allograft AcceptanceClinical & Developmental Immunology
EpigeneticsEpigenetic modulation of the immune function A potential target for toleranceEpigenetics
effector T cells; kidney transplantation; mesenchymal stromal cells; regulatory T cells; solid organ transplantation
© 2013 Lippincott Williams & Wilkins, Inc.
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