Despite downward trends in diarrhoeal mortality, there are still an unacceptably high number of child deaths annually. The principles of acute treatment are continued feeding, increased fluids (including oral rehydration solution), zinc supplementation and rational use of antibiotics. However, in a study from Dhaka, only 6% of caregivers of children with diarrhoea sought help from a qualified healthcare provider . Even when caregivers do seek appropriate help, healthworkers may have inadequate knowledge  and incorrect practices are common . In a survey of 264 healthcare workers in Indian slums , overuse of antibiotics and intravenous rehydration was widespread; practitioner knowledge strongly predicted correct practice, suggesting the need for ongoing caregiver and healthworker education.
Although most deaths arise from acute diarrhoea, a survey across seven countries found that persistent diarrhoea caused 30% or more infant diarrhoeal deaths in Ethiopia, Uganda, Tanzania, Pakistan and India . Over 40% of those dying of persistent diarrhoea were severely malnourished, highlighting the interactions between diarrhoea and malnutrition; it remains unclear why some children, but not others, develop persistent diarrhoea. GEMS reported more than eight-fold increased deaths among cases compared with controls 2–3 months after a single episode of MSD (OR 8.5, 95% CI 5.8, 12.5), highlighting the neglected post-discharge mortality associated with diarrhoea, particularly among malnourished children [2▪▪]. Immune function may also be impacted by diarrhoea. Leptin levels during acute cholera were low and stayed suppressed for at least 1 month after recovery; leptin concentrations on day 2 were related to immunoglobulin G antibody levels to cholera toxin 30 days later, indicating the impact of leptin on immune function . Together, these findings suggest that nutritional and immune convalescence need to be addressed to reduce post-diarrhoeal morbidity and mortality.
It has been debated how much diarrhoea contributes to growth failure in children. Overall, prior studies suggest a small but measurable effect on linear growth because of catch-up growth between episodes. A recent multicountry study  of 1007 children with longitudinal anthopometry and diarrhoeal surveillance from birth to 24 months confirmed that diarrhoea slows ponderal and linear growth, more in boys than girls. Faster (i.e. catch-up) length growth was observed during subsequent diarrhoea-free periods, confirming that catch-up growth can allow children to regain their original trajectory after short-term growth insults. Some pathogens may impair growth more than others; for example, in a Peruvian study , Shigella was particularly implicated. Although the reasons for reduced growth are multifactorial, a recent Zimbabwean study showed that diarrhoea can directly reduce circulating levels of insulin-like growth factor-1 .
It has been proposed that diarrhoea may adversely affect long-term neurodevelopment, although previous studies suggest this relationship arises predominantly through stunting. However, a recent study  of 422 Indian children, evaluated twice-weekly for illness and subsequently assessed for neurodevelopment using the Ages and Stages Questionnaire (ASQ-3), found that the number of diarrhoea days between 6 and 30 months was inversely associated with ASQ-3 scores, independent of growth. It is plausible that different pathways link diarrhoea with growth and brain development, although further mechanistic studies are needed.
Stunting is driven by complex interactions between genetics, epigenetics, environmental influences, recurrent infections and inadequate diet. A condition called environmental enteric dysfunction (EED), which is almost universal in impoverished settings, is also associated with stunting. EED is characterized by small intestinal inflammation and abnormal villous architecture, modest malabsorption and gut permeability; however, there is no case definition or gold standard biomarker and its cause remains unclear . A recent murine model [18▪▪] provides insights into the interactions between microbial exposure, enteropathy and malnutrition. Mice fed a suboptimal diet developed shifts in the small intestinal microbiota but retained normal intestinal histopathology; if they also received a bacterial cocktail they developed villous blunting and inflammation characteristic of EED. This supports the hypothesis in humans that EED arises from exposure to environmental microbes in conditions of poor sanitation and hygiene, particularly in the context of inadequate diet. Frequent enteropathogen carriage indicates that environmental contamination begins early in life. Exposure to faecal bacteria through geophagia  and contact with animal faeces  may be particularly important. The hypothesized causal pathway from EED to stunting is through malabsorption and chronic inflammation (arising from microbial translocation across an impaired gut barrier); however, this is difficult to confirm with current biomarkers . Recent studies using anti-endotoxin antibodies (EndoCAb) as markers of microbial translocation showed no relationships with growth in Malawi  or Zimbabwe , and plasma concentrations of intestinal fatty acid binding protein (indicative of villous damage) were elevated in Zimbabwean infants but not associated with stunting ; however, the role of chronic inflammation in stunting has been confirmed in several recent studies [22,23]. Dissecting the interactions between recurrent infections, impaired gut integrity, chronic inflammation and stunting will require more longitudinal studies, using panels of emerging biomarkers together with gut biopsy samples where feasible.
Postnatal gut development is highly influenced by changes in the microbiota and diet. A recent murine study [24▪] showed that many genes governing intestinal development are controlled by the microbiota, while dietary shifts at weaning led to changes in metabolic and antimicrobial gene expression, indicating that intestinal development is highly influenced by the environment. The gut needs to respond readily to pathogens, while avoiding inflammation in response to the microbiota. Through a bidirectional relationship, the microbiota can drive inflammation and the mucosal inflammatory milieu shapes the microbiota. However, the microbiota remains remarkably stable due to evolution of resilience mechanisms; for example, gut commensals are resistant to the activity of intestinal antimicrobial peptides, through mechanisms that are emerging . Mucosal inflammation is an important defence against enteropathogen colonization, but can also limit microbiota growth, counter-intuitively providing an advantage to pathogens that have evolved survival mechanisms . Key regulators of the interactions between inflammation and the microbiota are being identified . Immune cells, such as Th17, Th22 and γδ T-cells, have a critical role in intestinal homeostasis by producing interleukin (IL)-22, which maintains epithelial barrier integrity and regulates microbiota composition. IL-22-deficient mice have higher mortality than wild-type mice following Clostridium difficile infection due to translocation of commensals to extraintestinal organs, highlighting the importance of interactions between mucosal immune cells, intestinal barrier function and gut microbial composition in protection from pathogens .
The interplay between the microbiota and diarrhoeal pathogens was recently highlighted in a time-series metagenomic study of adults with cholera . Recovery was associated with a pattern of changes that recapitulate the original microbiota assembly seen in healthy children, indicating that certain taxa may promote repair of the microbiota ‘organ’. In this study, one species, Ruminococcus obeum, reduced Vibrio cholerae colonization . Similarly, a recent murine study  found that Clostridium scindens alone could confer colonization resistance to C. difficile infection, suggesting that protection from pathogens can be governed at the single species level. The protective role of the microbiota has raised concerns that perturbations of the gut community by antibiotics may impair colonization resistance. An observational study  of 465 children followed from birth in Vellore, India, found that those receiving antibiotics in the first 6 months of life had a 33% increased risk of diarrhoea through 3 years in adjusted analyses (incidence rate ratio 1.33, 95% CI 1.12, 1.57), although exclusive breastfeeding was protective, potentially due to beneficial bacterial species (e.g. lactobacilli) in breast milk. In the same cohort , children receiving antibiotics to treat diarrhoea had a subsequent diarrhoeal episode twice as soon as children not receiving antibiotics (median time ratio 0.50; 95% CI 0.38, 0.79). Although there is potential for unmeasured confounding, these studies suggest that antibiotics, particularly in young infants, may increase the risk of diarrhoea and shorten the interval between diarrhoeal episodes.
There are intriguing interrelationships between enteric and respiratory infections. For example, diarrhoea appears to increase the risk of subsequent pneumonia, possibly because of hypochlorhydria . Higher gastric pH may predispose to enteric infections through loss of the protective gastric acid barrier, and increase the risk of pneumonia via reflux of heavily colonized gastric contents. An elegant murine study [34▪] dissected a complex mechanism through which lung infections unexpectedly cause intestinal damage. Following intranasal infection with influenza, mice developed small intestinal damage, which was not caused by viral dissemination to the gut. Instead, lung-derived CCR9+CD4+ T cells homed to the small intestine and disrupted the microbiota through interferon-gamma secretion. In response to dysbiosis, the intestinal epithelium secreted IL-15, causing Th17 polarization of mucosal CD4+ T cells and IL-17-mediated gut damage. Thus, infections at distant sites may disrupt intestinal homeostasis through immune-mediated effects on the microbiota; further studies in humans are needed to explore these mechanisms further.
A series of recent studies highlights the role of the microbiota in malnutrition. By constructing a microbiota ‘maturity index’ based on age-discriminatory taxa that define a healthy pattern of bacterial assembly, maturational defects in the microbiota of children with severe acute malnutrition (SAM) were identified, which were only partially and temporarily restored by nutritional rehabilitation [35▪▪]. Gut microbes targeted by the mucosal immune system appear particularly important, because purified immunoglobulin A (IgA)-tagged bacteria from malnourished Malawian children transmitted a weight-loss phenotype to gnotobiotic mice, and IgA responses to certain taxa, including Enterobacteriaceae, correlated with child anthropometric measures . Using available metagenomic data in a secondary analysis, reduced microbiota diversity and changes in covariance network density were found to be associated with stunting severity in Malawi and Bangladesh, indicating a role of the microbiota in both linear and ponderal growth . It is now apparent that the community of gut viruses (virome) emerges after birth and interacts with the bacterial microbiota . Using machine-learning methods to characterize healthy assembly of the virome, children with SAM had a disrupted virome composition and, in contrast to the bacterial microbiota, community structure was not restored by therapeutic feeding .
There is an urgent need for new approaches and scale-up of existing interventions to reduce morbidity and mortality from diarrhoea, enteropathy and malnutrition. In a systematic review  of nonmedical interventions, such as infrastructure investments and behaviour change communication, most showed benefits ranging from 18 to 61% reduction in diarrhoeal incidence. The Global Action Plan for the Prevention and Control of Pneumonia and Diarrhoea outlines priority, low-cost, effective interventions to end preventable pneumonia and diarrhoea deaths by 2025 . A modelling exercise in South Africa showed that even 10% scale-up of 13 existing interventions for diarrhoea by 2030 would reduce under-5 diarrhoeal deaths by 48%; water, sanitation and hygiene (WASH), oral rehydration solution and exclusive breastfeeding would avert the majority of deaths .
Diarrhoea, pathogen carriage, microbiota composition and EED likely need to be addressed together to reduce malnutrition. Approaches focusing exclusively on feeding interventions, such as provision of lipid-based nutrient supplements, have only modest impacts on linear growth [48–50]. There is increasing appreciation that integrated approaches are needed; ongoing trials in Kenya (NCT01704105), Bangladesh (NCT01590095) and Zimbabwe  are evaluating the impact of combining WASH and feeding interventions to reduce stunting. Recent trials in children evaluating specific interventions for EED, including prebiotics (resistant starch type 2) , multiple micronutrients with or without fish oil  and zinc with albendazole , have shown little impact, although a recent trial  of multiple micronutrients in HIV-negative Zambian adults showed improved villous height and absorptive surface area on small intestinal biopsy after 6 weeks compared with placebo. A recent Kenyan study  used mesalazine to treat EED, similar to other inflammatory enteropathies. Children with SAM randomized to mesalazine, vs. placebo, had no excess adverse events and showed trends towards reduced inflammation after 28 days, providing evidence for larger efficacy trials of immunomodulation, although more potent agents targeting the small intestine in children without SAM may have greater efficacy.
Oral vaccines are the cornerstone of enteric infection prevention, but are least effective where they are most needed, possibly because of EED, enteric coinfections, malnutrition and interference from breast milk antibodies. Several strategies aimed at overcoming the oral vaccine effectiveness gap have recently been reported. In Pakistan , injectable poliovirus vaccine given with oral poliovirus vaccine (OPV) induced superior immune responses than OPV alone in well-nourished and malnourished infants. Withholding breastfeeding for 1 h prior to oral rotavirus vaccination paradoxically showed higher IgA seroconversion in the immediate feeding arm (37.8 vs. 28.2%; P = 0.07), although breast milk interference occurred in a subset of infants . A trial in Karachi  showed no improvement in serconversion with later or additional rotavirus vaccine doses. Further studies of alternative strategies are, therefore, needed to improve oral vaccine performance in settings with the highest enteric disease burdens.
Efforts to improve child survival and long-term developmental potential need to better understand and address the overlapping and interacting effects of diarrhoea, enteropathy and malnutrition. We believe that a pathological cycle emerges (Fig. 1), whereby intestinal pathogens cause dysbiosis and gut damage; the resulting chronic inflammation and malabsorption drive malnutrition, together with failed mucosal repair mechanisms, which render the child susceptible to further intestinal infections. Once established, this cycle may be difficult to interrupt; however, recent insights from human and animal studies suggest potential targets for intervention (Fig. 1) to reduce morbidity and mortality and improve the long-term potential of children in developing countries.
Papers of particular interest, published within the annual period of review, have been highlighted as:
1. Kovacs SD, Mullholland K, Bosch J, et al. Deconstructing the differences: a comparison of GBD 2010 and CHERG's approach to estimating the mortality burden of diarrhea, pneumonia, and their etiologies. BMC Infect Dis 2015; 15:16.
2▪▪. Kotloff KL, Nataro JP, Blackwelder WC, et al. Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet 2013; 382:209–222.
This major study used a careful case–control design to calculate pathogen-specific burdens of disease in young children with MSD in seven sites in Africa and Asia, showing that a few pathogens account for the majority of diarrhoeal disease.
3. Tellevik MG, Moyo SJ, Blomberg B, et al. Prevalence of cryptosporidium parvum/hominis, entamoeba histolytica and giardia lamblia among young children with and without diarrhea in Dar es Salaam, Tanzania. PLoS Negl Trop Dis 2015; 9:e0004125.
4▪▪. Platts-Mills JA, Babji S, Bodhidatta L, et al. Pathogen-specific burdens of community diarrhoea in developing countries: a multisite birth cohort study (MAL-ED). Lancet Glob Health 2015; 3:e564–e575.
This birth cohort study in three continents estimated pathogen-specific burdens of community diarrhoea and showed variation by age, geography and season, but with several pathogens accounting for the majority of disease.
5. Checkley W, White AC Jr, Jaganath D, et al. A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium. Lancet Infect Dis 2015; 15:85–94.
6▪. Vinayak S, Pawlowic MC, Sateriale A, et al. Genetic modification of the diarrhoeal pathogen Cryptosporidium parvum. Nature 2015; 523:477–480.
A new tractable platform for future experimental studies of cryptosporidiosis, including an optimized process for transfection of sporozoites in culture, a Cryptosporidium parvum clustered regularly inter-spaced short palindromic repeat (CRISPR)/Cas9 system, a mouse model that delivers sporozoites to the intestine and reporter parasites suitable for drug screening.
7. Chowdhury F, Khan IA, Patel S, et al. Diarrheal illness and healthcare seeking behavior among a population at high risk for diarrhea in Dhaka, Bangladesh. PLoS One 2015; 10:e0130105.
8. Lamberti LM, Fischer Walker CL, Taneja S, et al. The association between provider practice and knowledge of ORS and zinc supplementation for the treatment of childhood diarrhea in Bihar, Gujarat and Uttar Pradesh, India: a multi-site cross-sectional study. PLoS One 2015; 10:e0130845.
9. Carter E, Bryce J, Perin J, Newby H. Harmful practices in the management of childhood diarrhea in low- and middle-income countries: a systematic review. BMC Public Health 2015; 15:788.
10. Mahapatra T, Mahapatra S, Banerjee B, et al. Predictors of rational management of diarrhea in an endemic setting: observation from India. PLoS One 2015; 10:e0123479.
11. Rahman AE, Moinuddin M, Molla M, et al. Childhood diarrhoeal deaths in seven low- and middle-income countries. Bull World Health Organ 2014; 92:664–671.
12. Falkard B, Uddin T, Rahman MA, et al. Plasma leptin levels in children hospitalized with cholera in Bangladesh. Am J Trop Med Hyg 2015; 93:244–249.
13. Richard SA, Black RE, Gilman RH, et al. Catch-up growth occurs after diarrhea in early childhood. J Nutr 2014; 144:965–971.
14. Lee G, Paredes Olortegui M, Peñataro Yori P, et al. Effects of Shigella-, Campylobacter- and ETEC-associated diarrhea on childhood growth. Pediatr Infect Dis J 2014; 33:1004–1009.
15. Jones AD, Rukobo S, Chasekwa B, et al. Acute illness is associated with suppression of the growth hormone axis in Zimbabwean infants. Am J Trop Med Hyg 2014; 92:463–470.
16. Kvestad I, Taneja S, Hysing M, et al. Diarrhea, stimulation and growth predict neurodevelopment in young North Indian children. PLoS One 2015; 10:e0121743.
17. Prendergast AJ, Humphrey JH, Mutasa K, et al. Assessment of environmental enteric dysfunction in the SHINE trial: methods and challenges. Clin Infect Dis 2015; 61 (Suppl 7):S726–S732.
18▪▪. Brown EM, Wlodarska M, Willing BP, et al. Diet and specific microbial exposure trigger features of environmental enteropathy in a novel murine model. Nat Commun 2015; 6:7806.
This is the first animal model of environmental enteric dysfunction and identifies the factors that drive distorted intestinal architecture in an elegant series of experiments that advance our understanding of the pathogenesis of EED.
19. George CM, Oldja L, Biswas S, et al. Geophagy is associated with environmental enteropathy and stunting in children in rural Bangladesh. Am J Trop Med Hyg 2015; 92:1117–1124.
20. George CM, Oldja L, Biswas SK, et al. Fecal markers of environmental enteropathy are associated with animal exposure and caregiver hygiene in Bangladesh. Am J Trop Med Hyg 2015; 93:269–275.
21. Benzoni N, Korpe P, Thakwalakwa C, et al. Plasma endotoxin core antibody concentration and linear growth are unrelated in rural Malawian children aged 2-5 years. BMC Res Notes 2015; 8:258.
22. Prendergast AJ, Rukobo S, Chasekwa B, et al. Stunting is characterized by chronic inflammation in Zimbabwean infants. PLOS One 2014; 9:e86928.
23. Naylor C, Lu M, Haque R, et al. Environmental enteropathy, oral vaccine failure and growth faltering in infants in Bangladesh. EBioMedicine 2015; 2:1759–1766.
24▪. Rakoff-Nahoum S, Kong Y, Kleinstein SH, et al. Analysis of gene-environment interactions in postnatal development of the mammalian intestine. Proc Natl Acad Sci U S A 2015; 112:1929–1936.
In this analysis of global changes in intestinal gene expression, postnatal gut development is shown to be highly adaptable to changes in diet and microbiota composition.
25. Cullen TW, Schofield WB, Barry NA, et al. Gut microbiota. Antimicrobial peptide resistance mediates resilience of prominent gut commensals during inflammation. Science 2015; 347:170–175.
26. Maier L, Diard M, Sellin ME, et al. Granulocytes impose a tight bottleneck upon the gut luminal pathogen population during Salmonella typhimurium colitis. PLoS Pathog 2014; 10:e1004557.
27. Roberts ME, Bishop JL, Fan X, et al. Lyn deficiency leads to increased microbiota-dependent intestinal inflammation and susceptibility to enteric pathogens. J Immunol 2014; 193:5249–5263.
28. Hasegawa M, Yada S, Liu MZ, et al. Interleukin-22 regulates the complement system to promote resistance against pathobionts after pathogen-induced intestinal damage. Immunity 2014; 41:620–632.
29. Hsiao A, Ahmed AM, Subramanian S, et al. Members of the human gut microbiota involved in recovery from Vibrio cholerae infection. Nature 2014; 515:423–426.
30. Buffie CG, Bucci V, Stein RR, et al. Precision microbiome reconstitution restores bile acid mediated resistance to Clostridium difficile. Nature 2015; 517:205–208.
31. Rogawski ET, Westreich D, Becker-Dreps S, et al. The effect of early life antibiotic exposures on diarrheal rates among young children in Vellore, India. Pediatr Infect Dis J 2015; 34:583–588.
32. Rogawski ET, Westreich DJ, Becker-Dreps S, et al. Antibiotic treatment of diarrhoea is associated with decreased time to the next diarrhoea episode among young children in Vellore, India. Int J Epidemiol 2015; 44:978–987.
33. Leung DT, Das SK, Malek MA, et al. Concurrent pneumonia in children under 5 years of age presenting to a diarrheal hospital in Dhaka, Bangladesh. Am J Trop Med Hyg 2015; 93:831–835.
34▪. Wang J, Li F, Wei H, et al. Respiratory influenza virus infection induces intestinal immune injury via microbiota-mediated Th17 cell-dependent inflammation. J Exp Med 2014; 211:2397–2410.
This study identified novel interactions between respiratory infections and gut dysfunction, with lung-derived CD4+ T cells driving intestinal microbiota dysbiosis and gut inflammation.
35▪▪. Subramanian S, Huq S, Yatsunenko T, et al. Persistent gut microbiota immaturity in malnourished Bangladeshi children. Nature 2014; 510:417–421.
This study characterized normal postnatal assembly of the microbiota in well-nourished children and used a ’microbiota-for-age’ Z-score to show that this programme of assembly is impaired in children with severe acute malnutrition.
36. Kau AL, Planer JD, Liu J, et al. Functional characterization of IgA-targeted bacterial taxa from undernourished Malawian children that produce diet-dependent enteropathy. Sci Transl Med 2015; 7: 276ra224.
37. Gough EK, Stephens DA, Moodie EE, et al. Linear growth faltering in infants is associated with Acidaminococcus sp. and community-level changes in the gut microbiota. Microbiome 2015; 3:24.
38. Lim ES, Zhou Y, Zhao G, et al. Early life dynamics of the human gut virome and bacterial microbiome in infants. Nat Med 2015; 21:1228–1234.
39. Reyes A, Blanton LV, Cao S, et al. Gut DNA viromes of Malawian twins discordant for severe acute malnutrition. Proc Natl Acad Sci U S A 2015; 112:11941–11946.
40. Seguin M, Nino Zarazua M. Nonclinical interventions for acute respiratory infections and diarrhoeal diseases among young children in developing countries. Trop Med Int Health 2015; 20:146–169.
41. Qazi S, Aboubaker S, MacLean R, et al. Ending preventable child deaths from pneumonia and diarrhoea by 2025. Development of the integrated Global Action Plan for the Prevention and Control of Pneumonia and Diarrhoea. Arch Dis Child 2015; 100 (Suppl 1):S23–S28.
42. Chola L, Michalow J, Tugendhaft A, Hofman K. Reducing diarrhoea deaths in South Africa: costs and effects of scaling up essential interventions to prevent and treat diarrhoea in under-five children. BMC Public Health 2015; 15:394.
43. Ejemot-Nwadiaro RI, Ehiri JE, Arikpo D, et al. Hand washing promotion for preventing diarrhoea. Cochrane Database Syst Rev 2015; 9:CD004265.
44. Clasen TF, Alexander KT, Sinclair D, et al. Interventions to improve water quality for preventing diarrhoea. Cochrane Database Syst Rev 2015; CD004794.
45. Ercumen A, Naser AM, Unicomb L, et al. Effects of source- versus household contamination of tubewell water on child diarrhea in rural Bangladesh: a randomized controlled trial. PLoS One 2015; 10:e0121907.
46. Clasen T, Boisson S, Routray P, et al. Effectiveness of a rural sanitation programme on diarrhoea, soil-transmitted helminth infection, and child malnutrition in Odisha, India: a cluster-randomised trial. Lancet Glob Health 2014; 2:e645–e653.
47▪▪. Pickering AJ, Djebbari H, Lopez C, et al. Effect of a community-led sanitation intervention on child diarrhoea and child growth in rural Mali: a cluster-randomised controlled trial. Lancet Glob Health 2015; 3:e701–e711.
This cluster-randomized trial in rural Mali found improved growth and reduced stunting among children living in villages randomized to latrine promotion and construction, despite no reductions in diarrhoea or soil-transmitted helminth infections. This suggests that pathways other than diarrhoea may be impacted by WASH interventions.
48. Christian P, Shaikh S, Shamim AA, et al. Effect of fortified complementary food supplementation on child growth in rural Bangladesh: a cluster-randomized trial. Int J Epidemiol 2015; 44:1862–1876.
49. Hess SY, Abbeddou S, Jimenez EY, et al. Small-quantity lipid-based nutrient supplements, regardless of their zinc content, increase growth and reduce the prevalence of stunting and wasting in young Burkinabe children: a cluster-randomized trial. PLoS One 2015; 10:e0122242.
50. Maleta KM, Phuka J, Alho L, et al. Provision of 10-40 g/d lipid-based nutrient supplements from 6 to 18 months of age does not prevent linear growth faltering in Malawi. J Nutr 2015; 145:1909–1915.
51. SHINE Trial Team. A cluster randomized trial of the independent and combined effects of water, sanitation/hygiene and infant feeding on stunting and anemia in rural Zimbabwe: the SHINE Trial rationale, design and methods. Clin Infect Dis 2015; 61 (Suppl 7):S685–S702.
52. Ordiz MI, May TD, Mihindukulasuriya K, et al. The effect of dietary resistant starch type 2 on the microbiota and markers of gut inflammation in rural Malawi children. Microbiome 2015; 3:37.
53. Smith HE, Ryan KN, Stephenson KB, et al. Multiple micronutrient supplementation transiently ameliorates environmental enteropathy in Malawian children aged 12-35 months in a randomized controlled clinical trial. J Nutr 2014; 144:2059–2065.
54. Ryan KN, Stephenson KB, Trehan I, et al. Zinc or albendazole attenuates the progression of environmental enteropathy: a randomized controlled trial. Clin Gastroenterol Hepatol 2014; 12:1507–1513.e1501.
55. Louis-Auguste J, Greenwald S, Simuyandi M, et al. High dose multiple micronutrient supplementation improves villous morphology in environmental enteropathy without HIV enteropathy: results from a double-blind randomised placebo controlled trial in Zambian adults. BMC Gastroenterol 2014; 14:15.
56. Jones KDJ, Hunten-Kirsch B, Laving AMR, et al. Mesalazine in the initial management of severely acutely malnourished children with environmental enteric dysfunction: a pilot randomized controlled trial. BMC Med 2014; 12:133.
57. Saleem AF, Mach O, Quadri F, et al. Immunogenicity of poliovirus vaccines in chronically malnourished infants: a randomized controlled trial in Pakistan. Vaccine 2015; 33:2757–2763.
58. Ali A, Kazi AM, Cortese MM, et al. Impact of withholding breastfeeding at the time of vaccination on the immunogenicity of oral rotavirus vaccine: a randomized trial. PLoS One 2015; 10:e0127622.
59. Ali SA, Kazi AM, Cortese MM, et al. Impact of different dosing schedules on the immunogenicity of the human rotavirus vaccine in infants in Pakistan: a randomized trial. J Infect Dis 2014; 210:1772–1779.