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Cryptosporidiosis: an update in molecular epidemiology

Xiao, Lihuaa; Ryan, Una Mb

Current Opinion in Infectious Diseases:
Gastrointestinal infections

Purpose of review: Molecular tools have been developed to detect and differentiate Cryptosporidium at the species/genotype and subtype levels. These tools have been increasingly used in the characterization of the transmission of Cryptosporidium spp. This review addresses the most recent developments in molecular epidemiology of cryptosporidiosis.

Recent findings: The recent development of subtyping tools has led to better understanding of the population genetics and transmission of Cryptosporidium in humans. The population structure of C. parvum and C. hominis is apparently more complicated than previously suggested, with the likely existence of both clonal and panmictic populations. Thus, the transmission of C. parvum (genotype II) in humans is shown to be different in different areas, with zoonotic transmission important in certain places and anthroponotic transmission in others. The use of molecular tools has also led to the identification of geographic and temporal differences in the transmission of C. parvum and C. hominis, and better appreciation of the public health importance of other Cryptosporidium species/genotypes and the frequency of infections with mixed genotypes or subtypes.

Summary: Factors involved in the transmission of human cryptosporidiosis are difficult to examine using conventional methods. The use of molecular tools has been helpful in the assessment of the zoonotic potential of various Cryptosporidium spp. and sources of human infections, and has started to play a significant role in the characterization of transmission dynamic in endemic and epidemic areas.

Author Information

aDivision of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA and bSchool of Veterinary and Biomedical Sciences, Murdoch University, Western Australia, Australia

Correspondence to Lihua Xiao, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Bldg 22, Rm14, 4770 Buford Highway, Chamblee, GA 30341, USA Tel: +1 770 488 4840; fax: +1 770 488 4454; e-mail:

Abbreviations GP60: 60 kDa glycoprotein HSP70: 70 kDa heat shock protein 70 PCR: polymerase chain reaction RFLP: restriction fragment length polymorphism SSCP: single strand conformation polymorphism rRNA: small subunit ribosomal RNA

© 2004 Lippincott Williams & Wilkins, Inc.