It is estimated that half of the total people living with HIV/AIDS, or 15 400 000, are women. In sub-Saharan Africa, the region worst affected by the epidemic, 61% of HIV-infected individuals are women . Currently, condoms are the only effective method to prevent the sexual transmission of HIV; use of both female and male condoms requires male cooperation. To halt the continuing incidence of HIV/AIDS, women-controlled prevention methods are urgently needed. Topical microbides could provide an option for HIV prevention for women who are unable or unwilling to use condoms.
This article reviews and synthesizes microbicide-acceptability research published from November 2006 to March 2008. Although this review focuses on vaginal microbicides, the few available studies on rectal microbicides are also discussed.
Assessing acceptability: what is acceptability, how is it measured, and where?
Although microbicides offer a promising method of HIV prevention, their effectiveness at the population level will ultimately depend not only on their efficacy but also on their use. Acceptability of new reproductive health technologies has been defined as the ‘voluntary sustained use of a method in the context of alternatives’ . A conceptual model examining the sustained acceptability of microbicides posits that acceptability hinges on multiple levels: the individual woman, the couple, and ultimately the provider (or clinical trial) context .
Currently, there is no consensus regarding the definition and measurement of acceptability in microbicide research . Only one of the publications reviewed provided a specific definition of acceptability, as ‘the ability and willingness to correctly and consistently use a product’ [5•]. Yet, little information is available on consistent use in the reviewed publications, resulting in a focus on ability and willingness to use products in the recent literature. Moreover, few acceptability measures are comparable across studies, making the resulting contrasting findings across formulations and populations problematic.
Four methods have been used to assess microbicide acceptability. The first approach consists of measuring the acceptability of candidate microbicides in clinical trials [6•,7,8,9•,10–16,17•]. This type of acceptability measurement is often a secondary objective of phase I studies aimed at assessing product safety and, as such, have small sample sizes. The second is the assessment of acceptability of the use of surrogate products such as lubricants, vaginal moisturizers, spermicides or placebo gels [5•,18–21]. The third approach involves assessment of the prevalence of the use of microbicide-compatible products, such as douches and lubricants [22–25]. This method differs from the former in that, while the former involves an intervention study, the latter is purely observational, relying on participants' reports of product use. The fourth approach is the evaluation of acceptability using reports of intention to use hypothetical products [25–29].
Most studies published during the review period have focused on: (1) preferences for the physical characteristics of products (formulation, odor, color, packaging, stickiness, consistency, lubrication) [6•,9•,10,14,18,20,26,28,29]; (2) experience during product insertion (ease of use of product and applicator, comfort, leakage, volume, preference for timing of insertion, possibility for covert use) [5•,6•,7,9•,10,13–16,17•,18,20,21,26,28]; and (3) side effects (burning, itching, stinginess, pain, irritation, genital symptoms) [9•,12,14,18,26,28]. Ten studies have investigated acceptability during sexual intercourse, focusing on the effect of the product on sexual pleasure [6•,7,9•,10,13,14,16,17•,18,21]. Specific results are discussed below.
While some studies have used dual methods of quantitative and qualitative data in the form of focus groups or in-depth interviews to assess acceptability, most studies have relied on quantitative data only. Four studies incorporated qualitative data collection methods to assess the acceptability of candidate microbicides in clinical trials [6•,9•,10,14]. Rosen and colleagues [6•], have shown that supplementing quantitative data with qualitative research can provide insightful information on acceptability that quantitative data alone cannot capture. This includes specific information regarding situations in which participants might not use the product. Similarly, Morrow and Ruiz  have argued that qualitative data can explain quantitative findings by providing detailed information on the circumstances surrounding a particular event. Regardless of the method of data collection, however, all studies published to date have relied on small samples and covered short timeframes.
The majority of recent studies were conducted in the United States. Only six publications report acceptability research in Africa (three in South Africa, one in Zambia, one in Tanzania and one in Cameroon), only one in the Caribbean (Dominican Republic), only five are available on Asia from China, India and Thailand.
Acceptability among women
Not surprisingly, most microbicide-acceptability studies have been conducted among women. Presumably, a microbicide that makes sex at least as pleasurable as intercourse with no product is likely to have a positive impact on acceptability. Studies have shown that candidate microbicides either increased or had no effect on women's sexual pleasure. Most women in a US tenofovir gel trial [6•], a PRO2000 (Indevus Pharmaceuticals, Lexington, Massachusetts, USA) trial in India  a Carraguard (Population Council, New York, New York, USA) trial in Thailand [17•], a Carraguard trial in South Africa [9•], and a BufferGel Duet (ReProtect Inc., Baltimore, Maryland, USA) trial in the USA and Santo Domingo  reported that the product either increased or had no impact on sexual pleasure. On the other hand, in another Carraguard study conducted in Thailand, 70–78% of women reported that the gel had no impact on sexual pleasure and women enrolled in the Invisible Condom study in Quebec city also reported that the gel had no effect on libido [13,16]. It is encouraging that overall, there is no evidence of a negative effect on sexual pleasure. In the absence of sexual intercourse, over half those studied have reported that they like the product [8,11,12].
Reports of physical properties of microbicides during sex, however, have been less positive. Messiness, stickiness, leakage before, during, and after sex, and soiled clothes have been reported [6•,7,9•,13,14,16,17•]. Women have also found the volume of the product to be excessive or the product to be too wet [9•,16]. In terms of covert use, 70% of women in India said they would prefer a product that would not be noticeable during sex, while surprisingly only 26% of women in South Africa said they would use the gel without their partner's knowledge [9•,14].
Studies using microbicide surrogates corroborate findings from microbicide clinical trials. A preference for lower product volume has also been reported in a study of vaginal moisturizers in Zambia. Women reported that the most important factors in determining preference were the efficiency of the product, its ability to increase sexual pleasure, and its comfort and ease of use. Perceived partner's acceptability of products also affected women's preference .
The acceptability of hypothetical microbides among women has also been explored. Interestingly, two of these studies are among the few that investigated the role of contextual and interpersonal factors on acceptability. Willingness to use a hypothetical microbicide was associated with having non main partners in a US-based sample and having either only paying partners or both paying and main partners rather than a steady partner only among women in sex establishments in rural China [26,27]. In addition, women who believed that they had less control over their sex behaviors than their partners were more willing to use microbicides .
Additional factors associated with acceptability among women include single-use applicator design, effectiveness of product in preventing disease, and contraceptive properties . The importance of product characteristics was found to vary among women; they were less important among women of lower socioeconomic status and women with a history of douching . Similarly, in rural Southern China, women who use douches with applicators and antibiotic suppositories could be more predisposed to accepting microbicides .
Acceptability among adolescent girls
Three of the papers reviewed here examined microbicide-acceptability among sexually experienced adolescent girls aged 14–21 years in the United States. The first paper posits that mothers might be able to play a role in influencing their daughters' willingness to use microbicides . In the second study, after being shown two vaginal moisturizers, adolescents reported that they had a more favorable view of microbicides than condoms and perceived them to be ‘pleasurable’ and ‘comfortable’ . The major limitation of this study is that it relied on hypothetical products that the girls had not tried. The third paper reported that the use of vaginal moisturizers was associated with the product being comfortable, not leaking and not being messy . It is worth noting that sexually active adolescent girls who participate in such trials with parental consent might not be representative of the larger adolescent population.
Acceptability among heterosexual men
Although vaginal microbicides are designed to be used by women, the fact that men are exposed to them during sexual intercourse makes male acceptability an important area of research. Acceptability research conducted among heterosexual men falls into two categories: some studies investigated the acceptability of vaginal microbicides when used by women during intercourse while others investigated the acceptability of microbicides or microbicide surrogates when applied directly to the man's penis.
Five studies investigated the acceptability of microbicide candidates among male partners of women enrolled in clinical trials and, overall, studies reported that vaginal microbicides were acceptable to male sexual partners. However, messiness, stickiness, excessive wetness, excessive volume and leakage before, during and after sex have been reported [7,9•,10,16]. The impact of products on sexual pleasure varied [7,10,13,16].
A study assessing the acceptability of products applied on the men's genitalia found that men like the stickiness and slowness of drying of the gel the least. In addition, side effects were often reported. The most reported complaint was burning followed by tingling, and itching. The authors argue that in reality, the gel would not be applied directly to the penis, leading to less direct contact with the product and, therefore, the possibility for fewer side effects . On the other hand, in Kenya, three over-the-counter topical microbides designed to be used directly on the genitalia were highly acceptable to men, with 91–97% of men reporting that they would use the product again .
It has been suggested that a main advantage of vaginal microbicides is that they could be used covertly, without the male partners' knowledge. Some men, however, found it doubtful that these products could be used without the partner's knowledge mainly due to excess vaginal wetness [9•,10].
Acceptability among men who have sex with men
Acceptability of microbicides has been assessed among men who have sex with men (MSM) in the United States. It has been suggested that the high prevalence of lubricant use during anal intercourse and rectal douching before and after sex may facilitate the introduction of microbicides delivered as lubricant gels or harmless rectal douches in MSM populations [23,25]. Overall, men have responded positively to hypothetical microbicides and believed that microbides would be easier to use if formulated as lubricants . A study evaluating the volume of microbicide that would be acceptable to insert rectally before receptive anal intercourse reported that up to 35 ml of gel were deemed acceptable by most participants. Interestingly, volumes that were deemed acceptable without intercourse were not always acceptable during intercourse [5•]. This finding emphasizes the importance of assessing acceptability during sexual intercourse.
Although the recent research conducted among MSM provides useful information about the potential acceptability of microbicides in this population of high interest, it is worth noting that only one study [5•] evaluated the acceptability of an actual product, a placebo gel. The findings derived from these studies, although limited, set the stage for the first rectal candidate microbicides being currently tested. Although acceptability of rectal microbicides have only been assessed among MSM, when available, rectal microbicides could benefit both men and women who practice anal intercourse. Therefore, research is also needed on women's responses to rectal microbicides.
Evolution of microbicide acceptability across phases of product development
Recent research on microbicide acceptability has increased knowledge of aspects of use that may influence development of future microbicides. It is clear from the literature, however, that what may motivate individuals to use microbicides is a complex process that is likely to vary across social and cultural contexts. While Severy and Newcomer  introduced the importance of context in acceptability research, it has received little attention in the literature. Future acceptability research would benefit from investigating the contextual and interpersonal factors related to acceptability.
Findings derived from microbicide acceptability research are inherently limited because acceptability has only been assessed in the absence of a proven product, and using small samples of individuals within clinical trials, all of which may have an influence on acceptability. In clinical trials, study protocols often restrict the type of sexual intercourse participants can engage in. For heterosexual couples, protocols require that intercourse be limited to vaginal intercourse; couples have to abstain from oral or anal intercourse, and MSM are asked to practice anal intercourse only. In addition, for ethical reasons, study protocols mandate condom use. Therefore, in addition to the fact that rigid protocol mandates are likely to be violated, it is doubtful that acceptability findings obtained from these clinical trials will reflect ‘real world’ acceptability [5•,6•]. Acceptability may differ when individuals are able to report on product use within their full range of sexual habits.
With few studies conducted in sub-Saharan Africa and Asia and only one in the Caribbean, the geographic coverage of acceptability research is clearly not representative of the geographic need in terms of HIV prevention; especially in light of the fact that 96% of new HIV infections occur in low- or middle-income countries . Product preference and the social context surrounding acceptability are likely to be population-specific. The extent to which acceptability research conducted in the US can inform the development of products that would be of most use in the developing world remains unclear.
Figure 1 presents a conceptual model illustrating how as the next phase of research emerges, the context in which the candidate microbicides are evaluated will also change. The model lists factors that influence acceptability, adherence and continued use across the microbicide product development process, and eventually in the open market. Acceptability, as measured by single or short-term use of a product in a phase I or phase II trial, should be measured as adherence in phase IIb or phase III trials that require extended product use and as continuation/maintenance in the open market. Therefore, factors predicting adherence in phase IIb or phase III trials might differ from those that influence acceptability in phase I or phase II trials. Similarly, factors that influence continued use of an effective product in the open market might differ from those that predicted acceptability and adherence in trials. We present this model as a way of encouraging investigators to consider the next steps in understanding acceptability and to standardize measures to define acceptability as the context in which the research is conducted changes.
Until acceptability and sustained use of microbicides have been assessed within large, high-risk, and geographically diverse populations the potential effect of microbicides on the HIV epidemic will remain unknown.
In the next year it is expected that findings on acceptability from larger and longer duration clinical trials of microbicides will be available, moving this field into an exciting new phase. Ultimately, behavioral research on microbicides will need to tackle issues beyond acceptability, such as how perceived effectiveness may affect risk compensation behaviors (i.e. decreased condom use). This issue is particularly salient, as it is very unlikely that any microbicide will provide as much protection as do condoms against HIV.
References and recommended reading
Papers of particular interest, published within the annual period of review, have been highlighted as:
• of special interest
•• of outstanding interest
Additional references related to this topic can also be found in the Current World Literature section in this issue (p. 602).
1 UNAIDS/WHO. 2007 AIDS epidemic update. Geneva: UNAIDS/WHO; 2007.
2 Severy L, Newcomer S. Critical issues in contraceptive and STI acceptability research. J Soc Issues 2005; 61:45–65.
3 Severy LJ, Tolley E, Woodsong C, Guest G. A framework for examining the sustained accpetability of microbicides. AIDS Behav 2005; 9:121–131.
4 Mantell JE, Myer L, Carballo-Dieguez A, et al
. Microbicide acceptability research: current approaches and future directions. Soc Sci Med 2005; 60:319–330.
5• Carballo-Diéguez A, Exner T, Dolezal C, et al
. Rectal microbicide acceptability: results of a volume escalation trial. Sex Transm Dis 2007; 34:224–229.
6• Rosen RK, Morrow KM, Carballo-Diéguez A, et al
. Acceptability of Tenofovir Gel as a Vaginal Microbicide Among Women in a Phase I Trial: a mixed-methods study. J Womens Health 2008; 17:383–392. In this phase I clinical trial, both quantitative and qualitative data were collected to assess acceptability of tenofovir gel. The authors advocate the use of both methods in future trials as qualitative interviews can help explain acceptability data collected in structured questionnaires.
7 Ballagh SA, Brache V, Mauck C, et al
. A phase I study of the functional performance, safety and acceptability of the BufferGel Duet. Contraception 2008; 77:130–137.
8 Williams DL, Newman DR, Ballagh SA, et al
. Phase I safety trial of two vaginal microbicide gels (Acidform or BufferGel) used with a diaphragm compared to KY jelly used with a diaphragm. Sex Transm Dis 2007; 34:977–984.
9• Ramjee G, Morar NS, Braunstein S, et al
. Acceptability of Carraguard, a candidate microbicide and methyl cellulose placebo vaginal gels among HIV-positive women and men in Durban, South Africa. AIDS Res Ther 2007; 4:20. Acceptability of Carraguard was assessed among HIV-infected men and women in this phase I clinical trial using both structured and semi-structured in-depth interviews. This paper is among the few that examine the acceptability of a candidate microbicide among HIV-positive individuals as part of a clinical trial. As emphasized by the authors, there is a need to study acceptability among HIV-infected individuals as once microbicides are on the market they are likely to be used by individuals who do not know their HIV status.
10 Carballo-Diéguez A, Balán IC, Morrow K, et al
. Acceptability of tenofovir gel as a vaginal microbicide by US male participants in a phase I clinical trial (HPTN 050). AIDS Care 2007; 19:1026–1031.
11 Doh AS, Ngoh N, Roddy R, et al
. Safety and acceptability of 6% cellulose sulfate vaginal gel applied four times per day for 14 days. Contraception 2007; 76:245–249.
12 Barnhart KT, Rosenberg MJ, MacKay HT, et al
. Contraceptive efficacy of a novel spermicidal microbicide used with a diaphragm: a randomized controlled trial. Obstet Gynecol 2007; 110:577–586.
13 Trottier S, Omar RF, Désormeaux A, et al
. Safety, tolerance and acceptability of the Invisible Condom and its vaginal applicator in healthy women and their male sexual partners. Contraception 2007; 76:117–125.
14 Joglekar N, Joshi S, Kakde M, et al
, HIV Prevention Trial Network 047 Protocol Team. Acceptability of PRO2000 vaginal gel among HIV un-infected women in Pune, India. AIDS Care 2007; 19:817–821.
15 Jespers V, Buvé A, Van Damme L. Safety trial of the vaginal microbicide cellulose sulfate gel in HIV-positive men. Sex Transm Dis 2007; 34:519–522.
16 Kilmarx PH, van de Wijgert JH, Chaikummao S, et al
. Safety and acceptability of the candidate microbicide Carraguard in Thai Women: findings from a Phase II Clinical Trial. J Acquir Immune Defic Syndr 2006; 43:327–334.
17• Kilmarx PH, Blanchard K, Chaikummao S, et al
. A randomized, placebo-controlled trial to assess the safety and acceptability of use of carraguard vaginal gel by heterosexual couples in Thailand. Sex Transm Dis 2008; 35:226–232.
18 Jones DL, Weiss SM, Chitalu N, et al
. Acceptability of microbicidal surrogates among Zambian women. Sex Transm Dis 2008; 35:147–153.
19 Bukusi EA, Steele M, Cohen CR, et al
. Safety, acceptability, and tolerability of 3 topical microbicides among heterosexual Kenyan men. J Acquir Immune Defic Syndr 2007; 44:423–428.
20 Schwartz JL, Ballagh SA, Kwok C, et al
. Fourteen-day safety and acceptability study of the universal placebo gel. Contraception 2007; 75:136–141.
21 Short MB, Succop PA, Rupp R, Rosenthal SL. Adolescents' reasons for using a microbicide-like product over time. International Journal of Std and AIDS 2008; 19:115–117.
22 Nodin N, Carballo-Diéguez A, Ventuneac AM, et al
. Knowledge and acceptability of alternative HIV prevention bio-medical products among MSM who bareback. AIDS Care 2008; 20:106–115.
23 Carballo-Diéguez A, Bauermeister JA, Ventuneac A, et al.
The use of rectal douches among HIV-uninfected and infected men who have unprotected receptive anal intercourse: implications for rectal microbicides. AIDS Behav 2007; Aug 18 [Epub ahead of print].
24 Weeks MM, Abbott M, Liao S, et al
. Opportunities for woman-initiated HIV prevention methods among female sex workers in southern China. J Sex Res 2007; 44:190–201.
25 Carballo-Diéguez A, O'Sullivan LF, Lin P, et al
. Awareness and attitudes regarding microbicides and Nonoxynol-9 use in a probability sample of gay men. AIDS Behav 2007; 11:271–276.
26 Wang Y, Liao SS, Weeks MR, et al
. Acceptability of hypothetical microbicides among women in sex establishments in rural areas in Southern China. Sex Transm Dis 2008; 35:102–110.
27 Morrow KM, Fava JL, Rosen RK, et al
. Willingness to use microbicides varies by race/ethnicity, experience with prevention products, and partner type. Health Psychol 2007; 26:777–786.
28 Short MB, Perfect MM, Auslander BA, et al
. Measurement of microbicide acceptability among U.S. adolescent girls. Sex Transm Dis 2007; 34:362–366.
29 Morrow KM, Fava JL, Rosen RK, et al
. Willingness to use microbicides is affected by the importance of product characteristics, use parameters, and protective properties. J Acquir Immune Defic Syndr 2007; 45:93–101.
30 Morrow KM, Ruiz MS. Assessing microbicide acceptability: a comprehensive and integrated approach. AIDS Behav 2008; 12:272–283.
31 Cohen JA, Steele MS, Ureña FI, Beksinska ME. Microbicide applicators: understanding design preferences among women in the Dominican Republic and South Africa. Sex Transm Dis 2007; 34:15–19.
32 Sunder PK, Ramos S, Short MB, Rosenthal SL. Adolescent girls' communication with “mothers” about topical microbicides. J Pediatr Adolesc Gynecol 2006; 19:373–379.
© 2008 Lippincott Williams & Wilkins, Inc.