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Yang, Min; Hu, Xianwen; Lu, Xiaoye; Wu, Xiaobo; Yang, Zhengfei; Qian, Jie; Cahoon, Jena; Tang, Wanchun
Critical Care Medicine: December 2013
doi: 10.1097/01.ccm.0000439202.92457.f5
Oral Abstract Session: Cardiovascular / CPR: PDF Only

Introduction: The detrimental effects of epinephrine are closely related to its [beta]- and a1- action, which significantly increases the severity of post-resuscitation (PR) myocardial dysfunction. In the present study, we investigated the effects of [beta]- and [beta]- plus [alpha]1- adrenergic blocking agents on post-resuscitation myocardial dysfunction and myocardial injury biomarkers. Methods: Twenty-four male Sprague-Dawley rats weighing between 450 - 550g were randomized into 4 groups: 1) placebo; 2) epinephrine; 3) epinephrine with [beta]- blocker (propranolol) and; 4) epinephrine with [beta]- plus [alpha]1- blockers (propranolol and prazosin). Ventricular fibrillation (VF) was electrically induced. CPR was initiated after 8 mins of untreated VF and continued for 8 mins. Defibrillation was then attempted. Ejection fraction (EF) and myocardial performance index (MPI) were measured at baseline and at hourly intervals for 4 hrs after resuscitation. Troponin I (Tn I) and N-terminal pro-brain natriuretic peptide (NT-pro BNP) were measured at baseline and at 1 and 4 hrs after resuscitation by the ELISA kits. Results: The [beta]- and [beta]- plus [alpha]1- blockers significantly reduced the severity of PR myocardial dysfunction. EF and MPI were 60.23 +/- 1.23 and 1.01 +/- 0.06 in the [beta]- blocker group and 63.63 +/- 1.51 and 0.89 +/- 0.03 in the [beta]- plus [alpha]1- blockers treated group compared to 39.90 +/- 2.60 and 1.34 +/- 0.10 in the epinephrine treated group and 44.58 +/- 4.57 and 1.25 +/- 0.10 in the placebo group at PR 4 hrs (p<0.05). The [beta]- and [beta]- plus [alpha]1- blockers pretreatment also reduced the releases of Tn I and NT-pro BNP compared with the placebo and epinephrine groups at PR 4 hrs (2.4 +/- 1.0 and 1.8 +/- 0.6 vs. 8.2 +/- 5.2 and 9.5 +/- 6.9 ng/L, p<0.05; 153.7 +/- 44.9 and 151.7 +/- 24.3 vs. 241.8 +/- 16.9 and 298.9 +/- 118.5 ng/L, p<0.05). The [beta]- and [beta]- plus [alpha]1-blockers significantly improved the duration of survival (67.8 +/- 10.2 and 72.0 +/- 0 vs. 34.4 +/- 34.6 and 22.8 +/- 26.4 hrs, p<0.05). Conclusions: The [beta]- and [beta]- plus [alpha]1-blockers reduced the severity of PR myocardial dysfunction and attenuated release of myocardial injury biomarkers with improved duration of survival in a rat model of CPR.

(C) 2013 by the Society of Critical Care Medicine and Lippincott Williams & Wilkins