Institutional members access full text with Ovid®

11: IMPACT OF IMIPENEM TREATMENT ON COLONIC MYCOBIOTA IN RATS WITH DOUBLE-HIT SEPSIS

Guan, Jun; Liu, Shaoze; Lin, Zhaofen; Li, Wenfang; Liu, Xuefeng; Chen, Dechang

doi: 10.1097/01.ccm.0000424267.12760.8b
Oral Abstract Session

Shanghai Changzheng Hospital

the 281th hospital of PLA

Shanghai Changzheng Hospital

Abstract

Introduction: To investigate whether and how broad-spectrum antibiotic treatment changes the intestinal mycobiota in the context of sepsis.

Hypothesis: Our hypothesis was that imipenem treatment promote colonic overgrowth of commensal fungi and colonization of exogenous fungi in a rat model of severe sepsis.

Methods: We established a rat model of double-hit sepsis using burn injury and endotoxin challenge. Rats with burn injury or double-hit sepsis received imipenem treatment for 3 days or 9 days, and their colon contents were sampled for selective fungal culture and isolation counts.

Results: Our results demonstrated that imipenem treatment promoted the overgrowth of the commensal fungus Geotrichum capitatum in rats with burn injury. Imipenem treatment also promoted colon colonization by exogenous fungi in rats with burn injury and double-hit sepsis, including Trichosporon cutaneum, Candida albicans, Candida krusei, and Candida glabrata. A longer duration of imipenem treatment had a stronger impact on colon colonization by exogenous fungi.

Conclusions: We concluded that imipenem treatment facilitates the overgrowth of commensal fungi and colonization by exogenous, potentially pathogenic fungi in the colons of rats with burn injury or double-hit sepsis.

© 2012 by the Society of Critical Care Medicine and Lippincott Williams & Wilkins