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Current approaches to the diagnosis of bacterial and fungal bloodstream infections in the intensive care unit

Murray, Patrick R. PhD; Masur, Henry MD

doi: 10.1097/CCM.0b013e318270e771
Concise Definitive Review

Healthcare systems spend considerable resources collecting and processing blood cultures for the detection of blood stream pathogens. The process is initiated with the collection of blood cultures that depend upon proper skin disinfection, collection of an adequate number of specimens and volume of blood, and prompt processing in a sensitive culture system. Complementing blood cultures and gaining in use are techniques such as nucleic acid amplification tests and mass spectroscopy that allow clinical laboratories to detect and identify organisms from blood cultures substantially faster than conventional systems. Furthermore, certain resistance mutations can be detected within hours of organism detection, thus providing valuable guidance to clinicians who strive to initiate the appropriate antimicrobial therapy as rapidly as possible, and who wish to discontinue unnecessary drugs expeditiously. Molecular and mass spectroscopy techniques are changing sepsis diagnosis rapidly and will provide far more specific information far more quickly, but the performance characteristics of these systems must be understood by intensivists who use such information to guide their patient management.

From the Becton Dickinson Diagnostic Systems (PRM), Sparks, MD; and Department of Critical Care Medicine (HM), Clinical Center, National Institutes of Health, Bethesda, MD.

Patrick Murray is an employee of Becton Dickinson and has equity interest. Dr. Masur has not disclosed any potential conflicts of interest.

For information regarding this article, E-mail: hmasur@nih.gov

© 2012 by the Society of Critical Care Medicine and Lippincott Williams & Wilkins