Skip Navigation LinksHome > April 2013 - Volume 61 - Issue 4 > Extract of Clinopodium chinense Inhibits High Glucose–Induce...
Text sizing:
A
A
A
Journal of Cardiovascular Pharmacology:
doi: 10.1097/FJC.0b013e31827d2a08
Original Article

Extract of Clinopodium chinense Inhibits High Glucose–Induced Apoptosis in Human Umbilical Vein Endothelial Cells

Li, Juan MD*; Wu, Feihua PhD*; Chen, Kai MD; Liang, Jingyu MD; Ma, Shiping MD*

Collapse Box

Abstract

Abstract: Endothelial dysfunction has been observed in diabetes mellitus, which is related to hyperglycemia. Previous studies have shown that incubation of human umbilical vein endothelial cells (HUVECs) in high glucose causes a significant increase in apoptosis, possibly related to an increase in oxidative stress. The present study aims at investigating the protective effects of Clinopodium chinense extract (CCE) on high glucose–induced apoptosis and the underlying mechanisms. We show that treatment of CCE significantly increased cell viability and the activity of superoxide dismutase, reduced oxidative stress, and protected HUVECs against apoptosis induced by high glucose. Furthermore, our results demonstrate that the antiapoptotic effect of CCE was associated with the reduction of Bax expression and caspase-3 activity and the elevation of Bcl-2 expression. The decrease in caspase-3 activity and caused by CCE were blocked by LY294002, a specific inhibitor of phosphatidylinositol 3-kinase, the upstream kinase of Akt. Moreover, treatment of CCE on HUVECs resulted in dose-dependenent phosphorylation of Akt and inhibited the activation of NF-κB. Taken together, our results indicate that CCE could play an important role in protecting endothelial cell by inhibiting oxidative stress and modulating cell apoptosis signaling via the pAkt/NF-κB/Bax/Bcl-2/caspase-3 pathway, suggesting that it might be useful for the treatment of endothelial dysfunction–associated diseases such as vascular complications of diabetes mellitus.

© 2013 Lippincott Williams & Wilkins, Inc.

Follow Us!

Login

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.