In this study, we compared the effect of the essential oil of Citrus bergamia Risso [bergamot, bergamot essential oil (BEO)] on the intracellular Ca2+ levels in vascular endothelial (EA) and mouse vascular smooth muscle (MOVAS) cells, using the fura-2 fluorescence technique. BEO caused an initial transient increase in intracellular Ca2+ concentration ([Ca2+]i) in EA cells, followed by a decrease, whereas it induced a sustained increase in [Ca2+]i in MOVAS cells. Linalyl acetate (LA) as a major component of BEO-induced [Ca2+]i mobilization was similar to BEO in EA cells. The increase of [Ca2+]i by LA was higher in EA cells than in MOVAS cells. [Ca2+]i rise induced by extracellular Ca2+ application was significantly blocked by BEO or LA in EA cells but not in MOVAS cells, suggesting that BEO and LA block Ca2+ influx in EA cells. The present results suggest that BEO and LA differentially modulate intracellular Ca2+ levels in vascular endothelial and smooth muscle cells. In addition, blockade of Ca2+ influx by BEO and LA in EA cells may explain the protective effects of BEO on endothelial dysfunction associated with cardiovascular disease.
*Department of Basic Nursing Science, School of Nursing, Korea University, Seoul, Republic of Korea
†Department of Physiology and Biophysics, School of Medicine, Eulji University, Daejeon, Republic of Korea.
Reprints: Geun Hee Seol, PhD, Department of Basic Nursing Science, School of Nursing, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, Republic of Korea (e-mail: firstname.lastname@example.org); Sun Seek Min, PhD, Department of Physiology and Biophysics, School of Medicine, Eulji University, Daejeon 301-746, South Korea (e-mail: email@example.com).
The authors report no financial/conflicts of interest.
Received July 26, 2012
Accepted December 14, 2012