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Bradykinin B2 Receptor–Dependent Enhancement of Enalapril-Evoked Hypotension in Ethanol-Fed Female Rats

El-Mas, Mahmoud M PhD; Abdel-Rahman, Abdel A PhD

Journal of Cardiovascular Pharmacology: January 2011 - Volume 57 - Issue 1 - pp 72-78
doi: 10.1097/FJC.0b013e3181fef9e8
Original Article

Our previous studies showed that chronic ethanol feeding attenuates centrally (clonidine) evoked and potentiates peripherally (hydralazine) evoked hypotension in female rats. In this study, we investigated whether chronic ethanol (8 weeks, 5% wt/vol) alters hemodynamic responses elicited by angiotensin-converting enzyme (ACE) inhibition (enalapril) in telemetered female rats. Given the intimate interaction between ACE and bradykinin, studies were extended to investigate the role of bradykinin receptor (B2R) in ethanol-enalapril interaction. Compared with pair-fed controls, ethanol-fed female rats exhibited (1) higher renal expressions of ACE and B2R proteins and angiotensin II levels and (2) lower blood pressure. Pharmacological inhibition of ACE and B2R supports functional role for the higher levels of these 2 proteins in ethanol-fed rats because enalapril (10 mg/kg, intraperitoneally) caused significantly greater hypotensive response in ethanol-fed rats than in control rats. Further, blockade of B2R with bradyzide (2 mg/kg, intraperitoneally) abrogated the enhanced hypotensive effect of enalapril in ethanol-fed rats but had no effect on enalapril-evoked hypotension in control rats. Finally, enalapril enhancement of spontaneous baroreflex sensitivity (BRS) in control was absent in ethanol-fed rats. These findings demonstrate that chronic ethanol produces B2R-dependent enhancement of the hypotensive response elicited by enalapril and abrogates enalapril-evoked enhancement of spontaneous baroreflex response in female rats.

From the Department of Pharmacology, School of Medicine, East Carolina University, Greenville, NC.

Received for publication August 30, 2010; accepted September 27, 2010.

Dr. M.M. El-Mas was on a sabbatical leave from the Department of Pharmacology, Faculty of Pharmacy, Alexandria, University, Alexandria, Egypt.

Supported by grant R01 AA014441 from the National Institute on Alcohol Abuse and Alcoholism.

The authors report no conflicts of interest.

Reprints: Abdel A. Abdel-Rahman, PhD, Department of Pharmacology, School of Medicine, East Carolina University, Greenville, NC 27834 (e-mail: abdelrahmana@ecu.edu).

© 2011 Lippincott Williams & Wilkins, Inc.