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Efficacy of Grape Seed Proanthocyanidins on Cardioprotection During Isoproterenol-induced Myocardial Injury in Rats

Karthikeyan, K PhD*; Bai, B R Sarala PhD; Devaraj, S Niranjali PhD*

Journal of Cardiovascular Pharmacology: February 2009 - Volume 53 - Issue 2 - p 109-115
doi: 10.1097/FJC.0b013e3181970c01
Original Article

The present study was done to evaluate the role of grape seed proanthocyanidins (GSPs) in isoproterenol (ISO)-induced myocardial injury in rats. Male albino Wistar rats were pretreated with GSP (50, 100, and 150 mg/kg), 6 days a week, for 5 weeks. Induction of rats with ISO (85 mg/kg body weight, intraperitoneally) for 2 days resulted in a significant elevation of thiobarbituric acid-reactive substances in serum, mitochondrial cholesterol, triglycerides, and free fatty acids. A significant decrease was observed in serum reduced glutathione; ascorbic acid; α-tocopherol; ceruloplasmin; and mitochondrial cytochromes (b, c, c1, and aa3), phospholipids, and adenosine triphosphate. Pretreatment with GSP (100 and 150 mg/kg) positively altered the levels of all the parameters studied and restored normal mitochondrial function when compared with ISO-induced rats. The effect at a dose of 50 mg/kg was not promising when compared with the other 2 doses (100 and 150 mg/kg). These results confirm the efficacy of GSP in alleviating ISO-induced myocardial injury.

From the Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, India.

The authors report no conflicts of interest.

Received for publication June 24, 2008; accepted November 17, 2008.

Reprints: Dr. K. Karthikeyan, PhD, Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, India (e-mail: karthik_research@yahoo.com) and Dr. S. Niranjali Devaraj, PhD, Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, India (e-mail: niranjali@yahoo.com).

© 2009 Lippincott Williams & Wilkins, Inc.