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Cartilage oligomeric matrix protein in serum and epiphyseal plate in Kashin-Beck disease

Yan, Denglua; Song, Yanchengb; Pei, Fuxingc

Current Orthopaedic Practice:
doi: 10.1097/BCO.0b013e3182793dde
Original Research

Background: To investigate serum and epiphyseal plate expression of cartilage oligomeric matrix protein (COMP) in rats fed with Kashin-Beck disease (KBD)—affected diet.

Methods: Two hundred Wistar rats were divided into five groups. Group A was fed with a normal diet as control; group B was fed with a normal diet and trichothecene (T-2) toxin; group C was fed with a low nutrition diet and T-2 toxin; group D was fed with a low nutrition diet; and group E was fed with a KBD-affected diet. The serum bioactivity of COMP was tested by enzyme linked immunosorbent assay. The epiphyseal plate COMP messenger RNA (mRNA) was detected by reverse transcription–polymerase chain reaction (RT-PCR). Histological sections were stained with hematoxylin and eosin (HE).

Results: After 4 weeks, the epiphyseal plate showed more intense necrosis of chondrocytes in groups B, C, and E. The serum levels of COMP and epiphyseal plate COMP mRNA levels in group E at 12 weeks were the highest of all groups and had significant difference when compared with other groups (P<0.05). Compared with other groups, the variation trend of the serum levels of COMP and epiphyseal plate COMP mRNA levels in group C were similar to group E from week 1 week to week 12.

Conclusions: These findings demonstrate COMP metabolism in serum and epiphyseal plate expression were altered in KBD and may play a role as a potential biomarker for monitoring pathogenic progression of cartilage destruction of KBD.

Author Information

aShenzhen Nanshan Hospital, Guangdong Medical College

bThird Hospital of Nanfang Medical University

cWest China Hospital, Sichuan University

Funding: This research was funded by China national science & technology pillar program during the eleventh five-year plan period (2007BA125B04) and China national science foundation (81271937).

Financial Disclosure: The authors report no financial conflicts of interest.

Competing Interests: The authors have declared that no competing interests exist.

Correspondence to Fuxing Pei, MD, West China Hospital, Sichuan University, No. 37 Guoxue Lane, Chengdu, Sichuan 610041, China Tel: +862 885 422114; fax: +862 885 422114; e-mail:

© 2013 Lippincott Williams & Wilkins, Inc.